Firstly, the seeds and beads were prepared. For the Respirometer 1, 25 ml of water was put in a 50 ml graduated tube. Then 25 germinating peas were dropped into the graduated cylinder. In doing this the volume of the water went up to 29.5 ml. By subtracting the two volumes, the volume of the germinating peas were found it equalled 4.5 ml (29.5-25=4.5). That volume was recorded and the peas were removed onto a paper towel. For Respirometer 2, the graduated cylinder was refilled with 25 ml of water and 25 dry peas were added. Next, enough plastic beads were added to equal the volume of germinating peas for Respirometer 1. Then, the dry seeds and the bead were removed onto a paper towel. For Respirometer 3, the graduated cylinder was filled with 25 ml of water. Then, enough beads were added to equal the volume of germinating peas for Respirometer 1. The beads were then removed onto a paper towel. For Respirometers 4 through 6, the exact same procedure above was repeated however, instead of peas, corn was used. The volume of 25 germinating corn was 4 ml for respirometer 4. Therefore, the volume of 25 dry corn seeds and the beads for …show more content…
To start, an absorbent piece of cotton was placed at the bottom of each respirometer. Next using a dropping pipet, the cotton was saturated with 2 ml of 15% KOH solution. Next a small amount of non absorbent cotton was then put on top of the saturated one. This was to prevent KOH solution from coming in contact with the seeds. It is also crucial to get the amount of cotton and KOH solution the same for all of the respirometers. After this, 25 germinating peas were placed in Respirometer vial 1, 25 dry peas were placed in Respirometer vial 2, and the equivalent volume of beads in Respirometer vial 3. These step were repeated for the corn and beads for Respirometers 4-6. A rubber stopper with a calibrated pipet was put into each respirometer vial tightly so the respirometers did not
Then, for each of the 4 groups, a paper towel was folded in half, lined with the 20 seeds at the edge of the paper towel, and covered completely with approximately 12 milliliters of water. Then, the paper towels were rolled
Respiration was measured in germinating seeds in the Pea Lab because in germinating peas there is a high rate of cell respiration as the pea is still growing and needs to consume oxygen to continue growing. Pea plant cells rely on the process of cellular respiration to supply them with the energy that they need to stay alive and grow during germination. When the pea plants are grown the cells will still remove energy from sugar via cellular respiration; however, the sugar for cellular respiration will come from the process of photosynthesis instead of the stored
Meanwhile, the peas did carry out cellular respiration. Thus by correcting the readings from the peas with the readings from the beads, it would should the actual rate at which cellular respiration will occur in peas through not allowing changes in outside conditions (i.e. atmospheric pressure) affect the respiration rate. By correcting the readings, it will show the most accurate results under the conditions of the lab without any changes affecting the
The experiment that was used is cell respiration for the germinating lentils, germinated in the different liquids of water, sunny d juice, milk, cola and Gatorade. The materials used for this project was lentils, O2 sensor, CO2 sensor, lab quest, Gatorade ,milk, water, juice, cola, bio chamber and five plastic containers. One tablespoon of lentils was placed in a plastic container. The lentils were soaked in one cup of water for 24 hours. Following the 24 hours, the water was completely drained. The lentils were left untouched at room temperature for 12 hours. After 12 hours the lentils were rinsed in water, and again
In the lab, the Carbon Dioxide production in the germinating peas was significantly greater than the Carbon dioxide production in the non germinating peas, and the iced peas. This supported my hypothesis that the germinating Peas would undergo the most Cellular Respiration. The peas, in the cold water at 6 degrees Celsius ascertained my hypothesis by being higher than the non germinating peas. They were slower in Carbon dioxide production than the room temperature germinating peas because as the temperature drops, the molecules function slower, causing them to take in less Oxygen for energy. Because the peas that are at 23 degrees Celsius are warm, germinating, and ready to grow, they require more energy, resulting in more respiration. The non-germinating peas have the least Carbon Dioxide production because as they are dormant, and have no need for energy to grow. Our results are reliable, so farther experiments would include more trials between the room temperature germinating peas, and the iced germinating peas. This would provide a more accurate average rate of carbon dioxide increase, and decrease the errors. More extreme temperature between the peas would be tested to insure that the difference in gas production was a metabolic reaction. Another trial would to be to use other types of seeds to ensure that this reaction is not only sectionalised to pea
The procedure was repeated to prepare a second set of germinating peas, dry peas, dry peas plus beads and glass beads for use in the remaining respirometers.
Cell respiration is the most prevalent and efficient catabolic pathway. It require transfusions of energy from outside sources to perform their many tasks. The the objective of this experiment was to measure the concentration of oxygen gas and carbon dioxide gas. Also to determine whether germinating peas respire. The hypothesis would be that germinating
The black-eyed peas were all exposed to different amounts of lemon juice in each level. Level 1: 0%, level 2: 50%, and level 3: 100%. In level 1 by day 2, the number of black-eyed peas germinated increased greatly, but throughout the rest of the experiment it stayed the same. In both level 2 and 3, 0 seeds were germinated by day 2. During the time between day 2 and 6, level 2’s number of seeds increased from 0 to 8. In level 3 only 1 seed was germinated during that time. The graph supports that the seeds given less lemon juice had more seeds germinated.
1B). The tube with germinating peas had the heights rate of oxygen consumption. The reasoning behind this is because the peas were doing aerobic respiration in which the oxygen was consumed by the peas and the carbon dioxide was released from the peas. The thermobar tube had the lowest rate of oxygen consumption because the glass beads did not undergo aerobic respiration, rather potassium hydroxide reacted with carbon dioxide to form the potassium carbonate and water. In fact, the thermobar data displayed a negative slope of the amount raw oxygen consumption, showing that as the reaction continued that the amount of oxygen that was consumed decreased. In regards to the slopes, the scatterplot shows that there was a positive slope for the germinating
Methods: The experiment that was being tested was the germination of soybeans, the non-germination of soybeans and glass beads. First three vials were marked 1, 2 and 3. Then a small wad of dry cotton was placed into the bottom of the three vials.
The experiment began by undergoing a hand washing with antibacterial soap for a minimum of 90 seconds and thoroughly drying them. Afterward, six (6) Bolder paper towels were taken and torn at the perforated line. An individual towel was then folded 3 times [See figure one (1)]; This step was repeated five (5) more times. Then sixty (60) pea seeds were counted out and separated into ten (10) equal groups. The pea seeds when observed had a shrivels like coat with a dull army green color. Next was to measure the length of the sixty (60) seeds and then calculate the average by adding up all the measurements then dividing the sum by sixty (60); this was recorded in a table provided [See figure two (2)]. Then the folded paper towel was taken and one group (10) of pea seeds
The second test tube was filled with 2.5 ml of potato extract and 2.5ml of H20. The third test tube was filled with 1.25ml of potato extract and 3.75 ml of h20. The fourth test tube was filled with 0.625ml of potato extract and 4.35 ml of H20. These test tubes were put aside. Four more empty test tubes were then prepared.
All seeds were exposed to the same amount of temperature and light as they were all kept in the same location at the same times
At the ten minute mark the germinating seeds respired a total of 0.8 milliliters of carbon dioxide. At 15 minutes the germinating seeds respired 1 milliliter of carbon dioxide. At 20 minutes the germinating seeds respired 1.2 milliliters of carbon dioxide. After 5 minutes, the dry peas in respirometer #2 respired 0.19 milliliters of carbon dioxide. At ten minutes the dry peas had no change in the amount of respiration from the five minute mark. At 15 minutes the dry peas respired 0.26 milliliters of carbon dioxide. At 20 minutes the dry peas respired 0.36 milliliters of carbon
5. Weighing: Drag each ear on each of the plants to the weighing dishes to weigh its kernels. The yield of kernels of each plant is now on a weighing dish. Drag a weighing dish to the balance to read the weight of the kernels. Record the weigh of a Data Table. Proceed to weigh the kernel yield from all plants. Calculate the average kernel yield form each set of three identical plants that are grown under identical conditions from the same type of seed. Record the calculations on the Data Table.