1. Relate the chemical structure of an enzyme to its specificity and catalytic activity. 2. Design a quantitative research experiment to investigate the influence of pH or temperature on the activity of an enzyme. 3. Describe what information concerning the structure of an enzyme could be inferred from your experiments.

1. Relate the chemical structure of an enzyme to its specificity and catalytic activity. 2. Design a quantitative research experiment to investigate the influence of pH or temperature on the activity of an enzyme. 3. Describe what information concerning the structure of an enzyme could be inferred from your experiments.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Indicate whether each of the following characteristics of an enzyme is TRUE or FALSE. An enzyme. 1._____ is a biological catalyst 2.____ functions at a low pH 3.____ does not change the equilibrium position of a reaction 4._____ is obtained from the diet 5._____ greatly increases the rate of a cellular reaction 6._____ is needed for every reaction that takes place in the cell 7._____ catalyzes at a faster rate at higher temperatures 8._____ functions best at mild conditions of pH 7.4 and 37oC 9._____ lowers the activation energy of a biological reaction 10._____increases the rate of the forward reaction, but not the reverse. Match the common name of each of the following enzymes with the description of the reaction. a. dehydrogenase b. oxidase c. peptidase d. decarboxylase e. esterase f. Transaminase 1._____ hydrolyzes the ester bonds in triacylglycerols to yield fatty acids and glycerol 2._____ removes hydrogen from a substrate…
2. How does pH affect enzyme function? 1. pH level has no effect on enzyme function 2. A very high pH increases enzyme function 3. A very low pH decreases enzyme function 4. A specific pH range is required for an enzyme to function answer Why do you believe this is the correct answer?
1. Define optimum pH and temperature of an enzyme.2. How do changes in pH and temperature affect the native conformation of an enzyme?
1. What is the significance of the effect of enzyme concentration to enzyme activity in relation to the medical aspect? a. A person with fever cannot eat well and may suffer from indigestion b. A person with hyperacidicity experience vomiting upon taking milk c. Abnormal level of diagnostic enzymes indicate damage in the body tissues d. Too much hydrogen peroxide in the body forms free radicals if not decomposed by peroxidase 2. What is the relation of the effect of temperature on enzyme activity to the medical field? a. To explain the cause why a feverish person cannot eat b. To explain why drinking carbonated leaves a fizzing sensation in our tongue c. To explain the cause why milk should not be taken by hyperacidic individual d. To explain why bubbles are formed when cleaning wounds with hydrogen peroxide 3. What reaction was involved in the determination of Vitamin C content of fruits? a. Oxidation…
26. AN ORGANIC COFACTOR IN A CONJUGATED ENZYME?A. NEITHER VITAMINS NOR MINERALSB. MINERALS C. VITAMINSD. BOTH VITAMINS AND MINERALS 27. WHICH OF THE FOLLOWING STATEMENTS CONCERNING COFACTORS IS INCORRECT?A. VITAMINS CANNOT BE COFACTORSB. SOME COFACTORS ARE METAL IONS C. COFACTOR IS THE NON-PROTEIN PORTION OF ENZYME D. CONJUGATED ENZYMES CONTAIN COFACTORS 28. WHICH OF THE FOLLOWING VITAMINS IS NOT A FAT SOLUBLE VITAMIN? A. VITAMIN E B. VITAMIN C C. VITAMIN A D. VITAMIN D 29. WHAT HAPPENS TO SUBSTRATE MOLECULES AT AN ENZYME ACTIVE SITE?A. ALWAYS REACT WITH OXYGEN B. UNDERGO CHANGE TO A DESIRED PRODUCTS C. BECOME CATALYST C ALWAYS REACT WITH OXYGEN B UNDERGO CHANGE TO A DESIRED PRODUCT D. BECOME COVALENTLY BONDED TO THE ENZYME D. BECOME COVALENTLY BONDED TO THE ENZYME 30. ACTION OF CHEMICAL WARFARE AND ORGANOPHOSPHATE INSECTICIDE ARE BASED ON?A. REVERSIBLE ENZYME INHIBITOR B. IRREVERSIBLE ENZYME INHIBITOR C. REVERSIBLE ENZYME ACTIVATOR D. IRREVERSIBLE ENZYME ACTIVATOR
2. Characteristics of enzymes: a. One characteristic of enzymes is that they are reusable. This is important because b. Name other 3 characteristics of enzymes: i. i. i. 3. Naming enzymes a. Enzyme names end with Examples b. Enzymes are named after Examples
VII. Graph the details on the unknown enzyme then answer the questions below: Enzyme A Enzyme B pH Efficiency Efficiency 10 30 1 55 75 3 4 90 85 20 6 7 70 60 35 20 35 45 50 9 10 65 80 11 55 12 13 14 40 30 20 a. At what pH does enzyme A work best at? b. At what pH does enzyme B work best at? c. How high was the enzyme efficiency for enzyme A at a pH of 8? d. Which enzyme works best at a basic pH? e. Which enzyme is the most efficient at any pH?
1. What is your ideas about enzyme. 2. What are the applications of enzyme to our daily life.
1. Match the following words with their definitions. Product Active site Enzymes Catalyst Substrate Activation energy a. amount of energy required for a chemical reaction to occur b. substances that bring about a chemical reaction without being changed itself c. substances that enzymes act upon d. regions on the surface of enzymes that fit the substrate e. substance formed from the substrate at the end of the chemical reaction with an enzyme f. proteins that speed up a chemical reaction
1. What are enzymes? Describe briefly each class of enzyme according to its function. 2. What are the factors that affect enzyme activity? 3. How would you differentiate catalase from peroxidase?
4. A group of students is conducting a lab investigation on a chemical reaction. An enzyme is present during the chemical change, and the reaction rate is recorded at various temperatures. Their results are graphed below. What would be the most likely explanation of these results? @ The investigation was flawed and should be repeated. B The reaction is complete. © The enzyme was removed from the reaction. D The enzyme began to lose the ability to function at higher temperatures. temperature reaction rate
Compare the types of inhibitors and write any t hree applications of enzyme inhibitors in medicine Write the answer in detail in ur own words with least plagirism and with proper headings