A penicillinase inactivates a penicillin. An enzyme with Mw = 30,000 has only a single active site, kcat = 2,000 s-1 and KM = 5.10-5 M. In response to a treatment with 5 μmol of penicillin, a 1 mL suspension of bacteria releases 0, 5 µg of enzyme. A) What concentration of penicillin would cause the enzyme to react at half the maximum rate? B) A modified penicillin acts as a competitive inhibitor. If the affinity of E for penicillin and for modified penicillin are the same, what concentration of inhibitor reduces the rate of penicillin loss 5-fold with low Cs in the medium?
A penicillinase inactivates a penicillin. An enzyme with Mw = 30,000 has only a single active site, kcat = 2,000 s-1 and KM = 5.10-5 M. In response to a treatment with 5 μmol of penicillin, a 1 mL suspension of bacteria releases 0, 5 µg of enzyme. A) What concentration of penicillin would cause the enzyme to react at half the maximum rate? B) A modified penicillin acts as a competitive inhibitor. If the affinity of E for penicillin and for modified penicillin are the same, what concentration of inhibitor reduces the rate of penicillin loss 5-fold with low Cs in the medium?
Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter11: Chemical Kinetics: Rates Of Reactions
Section: Chapter Questions
Problem 81QRT
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A penicillinase inactivates a penicillin. An enzyme with Mw = 30,000 has only a single active site, kcat = 2,000 s-1 and KM = 5.10-5 M. In response to a treatment with 5 μmol of penicillin, a 1 mL suspension of bacteria releases 0, 5 µg of enzyme.
A) What concentration of penicillin would cause the enzyme to react at half the maximum rate?
B) A modified penicillin acts as a competitive inhibitor. If the affinity of E for penicillin and for modified penicillin are the same, what concentration of inhibitor reduces the rate of penicillin loss 5-fold with low Cs in the medium?
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