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- You are evaluating the kinetics of an enzyme catalyzed reaction containing 5.5 μM total enzyme and 11.2 μM substrate. At this substrate concentration, you determine that the Vo = 88.6 μmol mL-¹. s-¹. If the Vmax 833.3 mM s the KM is: . == " 30.9 μΜ 10.4 μΜ Ο 124.6 μΜ 234 μΜ 94.5 μMCalculate AG for the below balanced redox reaction as written. You may find a list of standard reduction potentials useful. 3 12 (s) + 2 Al (s) → 61 (aq) + 2 Al3+ (aq) O-4.24 x 102 kJ/mol O-6.48 x 102 kJ/mol O -2.12 x 102 kJ/mol O-1.27 x 103 kJ/mol O-6.37 x 102 kJ/molAn enzyme catalyzes a reaction with a Km of 9.50 mM and a Vmax of 2.10 mM · s-1. Calculate the reaction velocity, vo, for each substrate concentration. [S] = 3.25 mM vo : mM · s-1 [S] = 9.50 mM mM · s-1 Vo :
- The energy of activation for the reaction 2 HI – H2 + I2 is 180. kJ•mol-1 at 544 K. Calculate the rate constant using the equation k = Ae-EalRT. The collision diameter for HI is 3.5×10-8 cm. Assume that the pressure is 1.00 atm. 4.0 4.510e-27 X M-1.s-1What is time constant ? The steel ball bearings of 40 mm diameter and initially at uniform temperature of 600 °C are quenched in an oil bath maintained at 50 °C temperature. The heat transfer coefficient between the ball bearing and oil is 325 W/m2-K and the thermodynamics properties of the bearing can be taken as : k 45 W/m-K and thermal diffusivity a = 1.25 x 10-5 m/s. Determine : The time duration for which bearing must remain in oil to attain 225 °C temperature. b. The instantaneous heat transfer rate from the bearings when they are first immersed in oil and when they reach 225 °C.For the following results of Thermodynamics of Borax Solubility, the volume of Borax solution titrated by HCI is 8.00 mL. Table 1. Volumes of hydrochloric acid required to titrate a saturated borax solution at varying temperatures. The hydrochloric acid was a solution standardized at 0.2912 M. Borax Volume added (mL) Temp. (°C) 8.00 8.00 8.00 8.00 8.00 HCI Volume (mL) 50.5 33.75 40.7 27.02 30.0 17.95 20.2 13.43 10.3 8.55 Using Thermodynamic formula (R= 8.31 J/K•mol) and the above results, (e) AS° = (J/K⚫mol) Type your answer...
- A solution containing 0.00001 M ATP has transmission 70.2\% at 260 nm in 1 cm cuvette calculate the transmission of solution in a 3 cm cuvetteNeutral sphingomyelinase 2 converts sphingomyelin into ceramide and phosphorcholine. What kind of enzyme is it? Assume Vmax is 35 µM min-1. When you provide 3.0 x 10-5 M of sphingomyelin, you observe an initial velocity of 6.0 µM min-1. Calculate the KM.For the following results of Thermodynamics of Borax Solubility, the volume of Borax solution titrated by HCI is 8.00 mL. Table 1. Volumes of hydrochloric acid required to titrate a saturated borax solution at varying temperatures. The hydrochloric acid was a solution standardized at 0.2912 M. Borax Volume added (mL) Temp. (°C) 8.00 8.00 8.00 8.00 8.00 HCI Volume (mL) 50.5 33.75 40.7 27.02 30.0 17.95 20.2 13.43 10.3 8.55 Using Thermodynamic formula (R= 8.31 J/K⚫mol) and the above results, (b) What is the y-intercept of the best fitting line for this data? Type your answer...
- Calculate the reduction potential of cytochrome a3 (°' = 0.385 V) at 25.0°C when the Fe3+ form = 87.0 μM and the Fe2+ form = 25.0 μM. 0.440 V 0.353 V 0.417 V 0.387 VA particular enzyme has a ΔΔG‡ of -22.1 kJ mol-1 at 37.0 °C. Calculate the rate enhancement of this enzyme. (R = 8.3145 J mol-1 K-1)Calculate the Keq (report up to two decimal places and do not use scientific notation) for the net reaction at 298.15K. (see attached image) Note: R = 1.98 x 10 -3 kcal/mol-K