Compute for the appropriate amount (mL_) of the compositions of a 10 mL 2x CTAB buffer for DNA extraction. Answer D and E a. 2% w/v CTAB b. 1.4 M sodium chloride c. 100 mM Tris-Cl d. 20 mM EDTA e. 2% polyvinylpyrrolidone MW 40 000
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Compute for the appropriate amount (mL_) of the compositions of a 10 mL 2x CTAB buffer for DNA extraction. Answer D and E
a. 2% w/v CTAB
b. 1.4 M sodium chloride
c. 100 mM Tris-Cl
d. 20 mM EDTA
e. 2% polyvinylpyrrolidone MW 40 000
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- Compute for the appropriate amount (mL_) of the compositions of a CTAB NaCl solution for DNA extraction. a. 10% (w/v) CTAB b. 0.7 M NaClCompute for the appropriate amount (mL) of the compostions below of TE buffer used for DNA extraction. a. 10 mM Tris b. 1 mM EDTAAn extraction Buffer, EB(200 mM Trish-HCL(Ph 8), 250 mM NaCl, 25 mM EDTA, 0.5% SDS) is used for DNA extraction from cells. Use their stock solutions to prepare their respective working concentrations for the given working volume of EB. Stock Solutions are: 1M Tris-HCL(pH~8) 0.5M EDTA 0.8M NaCl 10% SDS Show final calculaed values in the table given below. Component 1L EB 500 ml EB 250ml EB 100ml EB 200mM Tris-Hcl 250mM NaCl 25mM EDTA 0.5% SDS dH2O
- Make 2 mL of 50 fold dilution of DNA solution and sodium phosphate buffer. DNA: 400 uL Sodium phosphate buffer, pH 6.9, 10mL - Calclate amount of DNA & buffer need to make dilutionFor a DNA sample, the OD value at 260nm = 0.125, and the OD value at 280nm 0.065. Purity of DNA - OD 260/280 %3D Concentration of DNA = Abs x 50 µg/ml x dilution factor (100) 0.125 x 50 µg/ml x 100 = Edit View Insert Format Tools Table 12pt v Paragraph v BIUA 2 T'vExpected 260/230 values for "pure" DNA are commonly in the range of
- t (5 a 1 dao) - Google Chrome edu.om/mod/quiz/attempt.php?attempt=2334320&cmid=1127808 SQU E-LEARNING SYSTEM (ACAI الوقت المتبقي 0.175mm .e O In the DNA extraction experiment, the separation of DNA from its packaging protein can be achieved by the addition of Detergent aO 1.00 Salt b O هذا السؤال Ethyl alcohol cO Papain powder .d O Cold water Le O In paramecium, osmoregulation occurs through 3 Activate Windowssk Macronucleus Goaotings to acttivate do nere to search 6. 70°F Mostly sunny 9:05 ENG 12/18 f2 O f3 O f4 O f6 O f8 end f7 0 F10 insert f9 D F12 T num IA break @ %24 & 8 2 3 r 4 5 6 7 8 A 9 9 R Y ! U S F G J K +|| 回 Q 以 4Fluorescence confocal microscopy (FCM) - STK38 monoclonal antibody (M01), clone 2G8- 1F3. a. b. 200 μm What is the basic principle of image formation using this microscopy technique? What can be observed and concluded from the image of the specimen?The concentration of a DNA sample is 40 nano grams per milliliter how many nanoliters will be needed to obtain 0.5 nano grams of DNA
- In a reverse phase chromatography set up, the component that yields the lowest Rf value is likely to be O Both polarity O Polar O Non-polar O Cannot be determinedA series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a Bradford assay. The increase in absorbance at 595 nm relative to the concentration of protein was plotted as shown below. Save 1.0- 0.8- 0.6- 0.4- 0.2- 0.04 0.0 0.5 1.0 2.0 2.5 1.5 BSA concentration (mg/mL) a) Using the graph, calculate the original concentration of protein present in a mixture of haemoglobin and methylene blue (diluted 1:50), which gave an absorbance of 0.20 at a wavelength of 595 nm using the Bradford assay. Show all workings. Absorbance (O.D.)What is the concentration of a DNA solution with an OD260 measurement of 3.175 if 1OD unit at 260nm represents a DNA concentration of 50 g/mL? Write the correct units.