Contrast for a specimen may be adjusted by _____. Question options: Adjusting the iris diaphragm Lowering the stage Turning off the substage condenser light source Changing the objective lens
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Contrast for a specimen may be adjusted by _____.
Question options:
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Adjusting the iris diaphragm
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Lowering the stage
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Turning off the substage condenser light source
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Changing the objective lens
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- Iris images are taken with normal visible wavelengthpictures, as well as pictures in the______ band.If you have chosen the objective lens with 10x and the ocular lens magnification is also 10x. Then the total magnification will be 100x. True FalsePreparing a wet mount of the letter "e”. 1. Obtain a prepared ‘e’ slide. 2. Focus the slide first with the scanning objective, then click to lower power and focus again. 3. Finally, focus the slide under high power. Remember, at high power, you should ONLY use the fine adjustment knob. 4. Draw the ‘e’ exactly as it appears in your viewing field for each magnification. The LPO, HPO, and Oil Immersion
- Preparing a wet mount of the letter "e”.1. Obtain a prepared ‘e’ slide.2. Focus the slide first with the scanning objective, then click to lower power and focus again.3. Finally, focus the slide under high power. Remember, at high power, you should ONLY use the fineadjustment knob.4. Draw the ‘e’ exactly as it appears in your viewing field for each magnification of Oil Immersion objectiveIn this image a stage micrometer and an ocular micrometer are aligned so that the 10 o.u. line aligns with the 100um line. The 200um line of the stage micrometer overlaps with the 90 o.u. line of the ocular micrometer. What is the calibration for this lens? eyepiece graticule 40 50 60 70 s0 9 100 stage micrometer 0.8 um/ou O 8 um/ou O 1.25 um/ou 0.25 um/ouTrue-False Once focus is achieved at one magnification, a higher-power objective lens can be rotated into position without fear of striking the slide.
- Match the lens (condenser, high-dry, low-power, ocular, or oil immersion) to its description.This lens, also known as the eyepiece, often comes in pairs.When you are using the 40X objective you should never use the: O iris diaphragm O fine focus coarse focus O pointerThe numerical aperture of the an objective lens: indicates magnification indicates the clarity indicates light capturing ability indicates the approprite use
- How does the numerical aperture of an objective lens affect resolution? Question options: Numerical aperture is the optical plane visible for each objective lens Numerical aperture decreases the amount of excess light on a specimen increasing the resolution and clarity Numerical aperture is the loss of light due to changes in density between the specimen and the objective lens Numerical aperture is a measure of the amount of light an objective lens can accept producing higher resolution and allowing smaller structures to be visualized with greater clarityThe lens closest to the specimen is called the______________.Match the lens (condenser, high-dry, low-power, ocular, or oil immersion) to itsdescription. Choices may be used more than once. This objective lens provides the lowest magnification.
