Determine the Ki for the inhibitor at 30 °Cand decide what type of inhibitor is being used.
Q: Suggest a simple method to determine whether a reaction is being inhibited by a competitive or…
A: Inhibitorsis a substance that reduces or decreases the activity of an enzyme. It inhibits the proper…
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Q: a compettitive inhibitor interferes with?
A: The enzyme generally binds with the substrate but in competitive inhibition, the inhibitor is…
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Q: describe the mechanism of each class of inhibitor, including how they impact the effective…
A: There are mainly four types of inhibitors . Competitive inhibitor Non-competitive inhibitor…
Q: what is the relative activity and the degree of inhibition caused by a competitive inhibitor when…
A: Enzymes are catalysts that enhance the rates of biochemical reactions (biological catalysts).…
Q: For the competitive, noncompetitive and uncompetitive inhibition type investigate following figures…
A: The enzyme inhibition is the regulatory mechanism of the enzyme. They are of two types, irreversible…
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A:
Q: Compare (linear) competitive inhibition with mixed inhibition, by referring to the similarities and…
A: solution In case of mechanism : 1) In competitive inhibition, binding of an inhibitor prevents…
Q: Define the term minimum inhibitory concentration and their uses.
A: Minimum inhibitory concentration (MIC) is the term used by microbiologists to test the minimum drug…
Q: True or False. If drug A produces a larger zone of inhibition than drug B on the Kirby-Bauer disk…
A: Introduction If drug A does produce a larger zone of inhibition, that is one positive attribute of…
Q: H. Draw a plot showing reaction velocity as a function of substrate concentration for Ks = 50 µM,…
A: As here given in the question, Vmax= 5uM S-1 Ks=50uM and Ki = 250uM both in presence and absence of…
Q: 78. Which situation(s) might result in a higher than expected plasma drug concentration? a. Enzyme…
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A: Diffusion is the process in which the gaseous molecules spread out and occupy the volume of the…
Q: How can you distinguish between a competitive inhibitor and an uncompetitive inhibitor…
A: Competitive inhibitor- In competitive inhibitor, an inhibitor that resembles the normal substrate…
Q: Use the plot provided to estimate Km and Vmax values for both with and without inhibitor of the…
A: Those proteins which help to speed up the chemical reaction are referred to as enzymes. The rate of…
Q: I have the substrate concentration as 20, 40 , 65 and the V(mol/min) no inhibitor 602, 690, 833 and…
A: MM plot is Michaelis menton plot that is constructed with Substrate concentration on x axis and Vo…
Q: From the active site diagram below, please indicate the cofactor from the indicated components. HO 4…
A: Cofactors are the non protein molecules that help the enzyme to carry out catalysis. Enzymes without…
Q: Determine Km and Vmax using Lineweaver-Burke in the presence and absence of inhibitor. What type of…
A: Michaelis menten constant, Km is the substrate concentration required to produce half maximum…
Q: Describe Bohr effect and give its physiological significance
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Q: Identify the two forms of mixed inhibition and briefly describe how the two forms differ?
A: The substance that inhibit the activity of enzymes are called enzyme inhibitors and the mechanism by…
Q: what is the relative activity and the degree of inhibition caused by a competitive inhibitor when…
A: Enzymes are biocatalyst that increases the speed of reaction by lowering the activation energy.…
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Q: Classify the enzyme inhibitors by giving the names, mechanisms, and effect on the kinetic parameters…
A: Enzymes are bio-catalyst. They increase the speed of reaction by lowering its activation energy.…
Q: Given the active site diagram below, identify the mechanism(s) of catalysis. 5 2 Metal ion Induced…
A: active site is a region on an enzyme that binds to a protein or other substance during a reaction.
Q: Determine whether or not reaction is a redox reaction. For each redox reaction, identify the…
A: Redox reaction is considered as a process, during which one element is must be reduced or oxidized…
Q: Given the active site diagram below, identify the mechanism(s) of catalysis. 2 1 5 Metal ion Induced…
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Q: Which statement is consistent with these data? Compound X is an allosteric activator and Compound Z…
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A: Chymotrypsin is a seine protease. Serine protease is a class of enzyme in which catalytic mechanism…
Q: 460 410 360 310 260 210 160 110 60 -10 -S10 5 10 15 20 25 30 35 40 45 50 55 1/IS] per mM 1. What…
A: Enzymes are biocatalyst that are responsible for performing specific reaction within the body. They…
Q: Describe non-competitive inhibition.
A: Enzymes are protein that increases the rate of reaction by lowering the activation energy. Enzymes…
Q: Calculate the Km of the enzyme with these parameters. kcat = 130s^-1 Vo = 3.0 μMs-1 S = 10 μM…
A: Km of an enzyme is known as Michalis constant, which denotes the substrate concentration at which…
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Q: How would a competitive inhibitor change the Km and the Vmax for an enzyme? Explain why a…
A: A competitive inhibitor is interruption of a chemical pathway owing to one chemical substance…
Q: The following data were obtained in the presence and absence of inhibitor. Rate without Rate with…
A: Enzyme inhibition refers to the decrease in enzyme related processes, enzyme production, and enzyme…
Q: Write the difference between compititive and non compititive enzyme inhibitors.
A: Enzyme inhibitors binds with the active sites of enzymes and reduces the compatibility of substrate…
Q: For which purpose the method of measuring biochemical demand is used?
A: Most natural waters contain small quantities of organic compounds. Aquatic microorganisms have…
Q: Calculate KI' of the inhibitor from the information given. All information may not be needed to…
A: Enzyme inhibition refers to the decrease in enzyme related processes which may be enzyme production…
Q: Calculate the specific activity and kcat for this enzyme
A: Enzymes are catalyst that speed up the biochemical reaction by lowering the activation energy of the…
Q: Explain why structure 4 is a more potent inhibitor (lower IC50 value) than inhibitor 3 and why…
A: IC50 is the total concentration of inhibitor that is required to reach 50% inhibition. This means…
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- The biexponential equation for a two compartment model drug is C1,t = 50.0e-1.64t + 20.0e-0.147t. The drug is administered as a rapid IV bolus at t = 0. After 2.00 hours how many mg/l will the drug concentration in the central compartment be.rug-B has an elimination half-life of 9 hours, an absorption half-life of 20 minutes and linear harmacokinetics. In a patient, the plasma concentration five hours from the administration of two 60 mg cablets of the drug is 8 ug/mL. What would you expect to be the plasma concentration five hours from the administration of one 60 mg tablets? 1 ug/mL Answer 1-1A patient requires 2200 kcal/day from D50 (50% dextrose) and 8.5% aa (initial concentration). What is the hourly final goal rate of TPN? Group of answer choices 45 ml/hr 90 ml/hr 110 ml/hr 75 ml/hr
- Data for standard glucose concentrations Glucose concentration (mm) 0 0.5 1 1.5 -~~ 2 2.5 A Data for plasma samples B Patient Timepoint (hours) C D E 0 0.5 1 2 0 0.5 1 2 0 0.5 1 2 1. Draw a standard curve of absorbance at 420nm against glucose concentration in mmoles/L. We recommend you use Excel or a similar programme to insert the graph Alternatively, you can draw by hand on graph paper and insert a photo of the graph. OON 0 0.5 1 Absorbance 1 0.000 0.477 0.947 1.234 1.733 2.160 2 0 0.5 1 Absorbance Absorbance 2 0.000 0.482 0.941 1.236 1.721 2.276 Absorbance Glucose concentration Glucose concentration Abs 1 Abs 2 Average from graph (mM) in plasma (mm) 0.635 0.632 0.629 1.368 1.370 1.369 1.382 1.388 1.385 1.151 1.147 1.149 0.566 0.844 0.869 0.772 0.395 0.397 0.399 0.850 0.858 0.854 0.738 0.744 0.741 0.539 0.531 0.535 0.776 0.780 0.778 1.475 1.481 1.478 1.446 1.444 1.445 1.302 1.296 1.299 0.560 0.563 0.848 0.846 0.871 0.870 0.774 0.773 Average 0.000 0.480 0.944 1.235 1.727 2.218 0.440…Insulin Lispro subcutaneously for a patient weight 80 kg for 1 month of treatment. Daily dose of insulin is 1 Unit/kg and 60% in short acting, given 4 times per day; ampoules in form of personal injectors (300 Units/3ml). (I would like to figure out the number of personal injector needed for 1 month.) My answer: Daily dose (DD) for a 80 kg patient = 80 x 1 Unit = 80 U 60% is short acting, so DD = 60% of 80 U = (0.6 × 80) U = 48 U Given 4 times per day, so for a single dose = 48 U/4 = 12 U For 1 month (30 days), 30 x 48U = 1440 U Rp. Insulin lispro in ampoules personal injectors 300 Units/3 ml No. ??? (my teacher write is 10 but I don't understand why) Sig. Inject subcutaneously 12 Unit (0.12 ml) before each meal, 4 times a day for 1 month.Consider the following release data for a drug in water Q (Cumulative amount released in mg/cm?) Time (min) 15 12 30 16 45 20 60 23 120 32 240 46 480 65 1. On MS Excel , Plot Q Vs t, and Q Vs vt in separate graphs. Determine the equation and R2 for each curve. solve Q2 only show me by steps 2. Based on the R for the graphs, which release pattern does the drug follow, and why?
- Answer the questions based on the data table. [s] mM 0.333 0.40 0.50 0.666 1.0 2.0 V (M/s) Uninhibited 1.65 x 107 1.86 x 107 2.13 x 107 2.49 x 107 2.99 x 107 3.72 x 107 V (M/s) x 107 Inhibitor A 1.05 x 107 1.21 x 107 1.43 x 107 1.74 x 107 2.22 x 107 3.08 x 107 Determine the Km and Vmax of the enzyme. Determine the type of inhibition imposed by inhibitor A. Determine the type of inhibition imposed by inhibitor B. V (M/s) x 107 Inhibitor B 0.794 x 107 0.893 x 107 1.02 x 107 1.19 x 107 1.43 x 107 1.79 x 107What is the experimentally-determined minimum inhibitory concentration of the antibiotic being tested in this picture, based on the results shown in the picture? 0.06 0.125 0.25 1.0 μg/ml 8.0 µg/ml 0.5 µg/ml ○ 0.06 µg/ml µg/ml 0.5 1.0 2.0 4.0 8.0You are requested to recommend a dosage regimen for a patient (5S years old, 80 kg) to achieve plasma levels of 25 mg/L of an antiepileptic agent with an elimination half-life of 11 hrs and an apparent Vd of 0.02 L/kg. The tablets are 75% bioavailable and are available as 10 mg and 40 mg tablets. The ideal dosage regimen for this patient is: 10 mg twice daily 10 mg four times a day 40 mg daily 40 mg twice daily
- IV D5W/NS with 20 mEq KCL 1,000 mL/8 hr Allopurinol 200 mg PO tid Fortaz 1 g IV q6h Aztreonam (Azactam) 2 g IV q12h Flagyl 500 mg IV q8h Acetaminophen two tablets q4h prn A.Calculate mL/hr to set the IV pump. B. Calculate how many tablets of allopurinol will be given PO. Supply: 100 mg/tablet. C. Calculate how many mL/hr to set the IV pump to infuse Fortaz. Supply: 1-g vial to be diluted 10 mL of sterile water and further diluted in 50 mL NS to infuse over 30 minutes. D. Calculate how many mL of aztreonam to draw from the vial. Supply: 2-g vial to be diluted with 10 mL of sterile water and further diluted in 100 mL NS to Infuse over 60 minutes. E. Calculate how many mL/hr to set the IV pump to infuse Flagyl. Supply: 500 mg/100 mL to infuse over 1 hour.A%252F%252Fbbniagaraccc.sln.suny.edu%252Fwe ignment Saved The drip rate of a dobutamine IV depends on the dose (in mcg/min), the weight of the patient, and the dobutamine solution concentration (in mg/ml). For a concentration of 2 mg/ml, the drip rate (in ml/hour), is described by the following formula: Drip rate = 0.03 [patient body mass (kg) × dose infusion rate (mcg/kg-min)] For a patient weighing 209 pounds and a dose infusion rate of 20 mcg/min for every kilogram of body mass, what is the IV drip rate? ml/hrA 50-year-old diabetic comes to the laboratory to have rou- tine bloodwork drawn. His physician orders basic metabolic panel and a lipid profile. After the blood clots, the techni- cian spins down the specimen to find that the serum looks like milk. The test results are as follows: Na: 140 mEq/L K: 4.0 mEq/L Cl: 101 mEq/L CO2: 28 mg/dL Glucose: 455 mg/dL BUN: 24 mg/dL Creatinine: 1.1 mg/dL Phosphate: 3.7 mg/dL Calcium: 8.8 mg/dL ALP: 250 IU/L AST: 35 IU/L ALT: 29 IU/L Cholesterol: 500 mg/dL Triglycerides: 1200 mg/dL HDL: 35 mg/dL LDL: 160 mg/dL What causes the diabetic's serum to look like milk? What lipid test results do you expect to be elevated? How would this specimen fit into Fredrickson's classification scheme?