EcoRIBamHIXhol+ ||| | || + || | +++ |+DNAsize+ladder1*The same total amount of DNA was loaded in each lane.3000 bp2500 bp-2000 bp-1500 bp1200 bp1000 bp900 bp800 bp700 bp600 bp500 bp400 bp300 bp200 bp100 bpVC 100bp Plus DNA ladderfrom Vivantis Technologies

Approach to solving the question: Detailed explanation:Based on the image you sent, it appears to be…

Answered: EcoRI BamHI Xhol + || | | | | + | | | +…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

A DNA synthesizer “machine” is used to create short single stranded DNA of any given sequence. You have used the machine to create the following the DNA molecules: (DNA #1) 5’- CTACTACGGATCGGG – 3’ (DNA #2) 5’- CCAGTCCCGATCCGT – 3’ (DNA #3) 5’-AGTAGCCAGTGGGGAAAAACCCCACTGG-3’ Now you add the DNA molecules either singly or in combination to reaction tubes containing DNA polymerase, dATP, dCTP, dGTP, and dTTP in a buffered solution that allows DNA polymerase to function. For each of the reaction tubes, indicate whether DNA polymerase will synthesize any new DNA molecules, and if so, write the sequence(s) of any such DNAs. DNA #1 plus DNA #3 DNA #2 plus DNA #3 DNA #1 plus DNA #2 DNA #3 only
EcoRI recognizes G A-A-T-T-C sequence and cleave/ cut between G and A. How will the DNA fragments look like if EcoRI is used for the DNA below? How many fragments are produced? 5- AAAGATTTGAATTTCGAATTCAATTTAAGAATTCCCTTAGAATTTCC -¹3
Which of the following statement(s) is/are false/incorrect? You may select multiple options, if necessary. ☐ Experiments support bidirectional movement of multiple replication forks at eukaryotic chromosomes. O Mg++ ions play important roles during DNA synthesis Synthesis of DNA is semi-conservative ✔ Beta clamp reduces DNA polymerase processivity ✔ DNA polymerase III uses ATP as a source of energy to drive the polymerization reaction forward O Tautomers enhance chances of correct nucleotide incorporation during DNA synthesis
Questions are talking about amplification of DNA When we run the gel, we also run a ladder why? what information does that give us. why does it form a ladder shape on the gel (what is happening to cause things to separate out in many bands)?
Matching Type Choose the directionality of the given process. (4 points) What is the directionality of the given process? * 4 points 3'-5' 5'-3' Exonuclease activity Complementary strand of the continuous strand Addition of nucleotides going to the replication fork Addition of nucleotides away from the replication fork
Explain why the absorption of UV light by double-stranded DNA increases (the hyperchromic effect) when the DNA is denatured.
DNA ladder 1 DNA ladder 2 bp bp ng/Sul 10000 40 8000 40 8888 180 4000 40 -600 3000 40 500 2000 100 1500 40 1000 40 500 40 200 100 5 HILane. 8 cm Gel 0.5 ug/lane, 20 cm length gel, 1X TAE, B V/cm, 3h Please state the band length of each well (if exist) via comparing them with DNA ladder Please write if there is any unusual band pattern (smear, primer dimer). 1% agarose
Which of the following things must happen first if DNA is going to be replicated * DNA gets broken into smaller pieces to make the process more manageable DNA gets unzipped, so the two complimentary strands are separated DNA Polymerase adds on bases that match with the original strand DNA gets wound back into chromosomes so it's ready for mitosis Lenovo esc -> 女 # 2$ 2 3 5 6 7 8 9. tab e r t y a f k ift V alt ctri
TRANSLATE this RNA sequence: AUGCAAUGA Met-Gln-Stop Met-His-Stop Thr-Glu-Stop Thr-Pro-Stop What would happen if the nitrogen base second to the last of the sequence will undergo a point mutation, (G turning to A) Met-Gin-Stop Met-His-Stop Thr-Glu-Stop Thr-Pro-Stop The DNA sequence ATCAGCGCTGGC is part of a gene. how many amino acids are coded for by this message? O 4 8. 12 20 Option 5 For the lab part: how is DNA used for catching crime suspects. Describe the procedure and cite a particular example where it helped solve a case or absolved an innocent person from any wrongdoing. Your answer O O
An Investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42 C, she discovers that Okazaki fragments accumulate in unusually large quantities. What is the likely target affected by the temperature-sensitive mutation? DNA gyrase DNA polymerase II O DNA Ligase O primase DNA helicase
During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricity
EcoRI --- 5' G - AATTC 3' 5' AGAATTCCGACGTATTAGAATTCTTAT CCGCCGCCGGAATTCT CATCA 3' 3' TCTTAAGGCTGCATAATCTTAAGAATAGGCGGCGGCCTTAAGAGTAGT 5' Number of pieces of DNA , and type of fragment .

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EcoRI BamHI Xhol + || | | | | + | | | + ++ | + DNA size + ladder 1 *The same total amount of DNA was loaded in each lane. 3000 bp 2500 bp -2000 bp -1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp VC 100bp Plus DNA ladder from Vivantis Technologies