enzyme has a molecular weight 5 X 10'daltons, a KM value and a k₂ value of k₂=10* molecules ATP/min molecule enzyme at 37 C. The reaction catalyzed is the following ATP → ADP + P This can also be represented as: E + S K₁ K₁ E-S K₂ E + P Here S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: E = Ege-kat Here Eo is the initial enzyme concentration and ka=0.1 min¹. In an experiment with a partially pure enzyme preparation, 10 µg of total crude protein (containing enzyme) is added to a 1 mL reaction mixture containing 0.02 M ATP and incubated at 37C. After 12 hours, the reaction ends (i.e., t→∞) and the inorganic phosphate (P) concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: since [S]>>>Km, the reaction rate can be represented by: d (P) dt = K₂ [E]
enzyme has a molecular weight 5 X 10'daltons, a KM value and a k₂ value of k₂=10* molecules ATP/min molecule enzyme at 37 C. The reaction catalyzed is the following ATP → ADP + P This can also be represented as: E + S K₁ K₁ E-S K₂ E + P Here S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: E = Ege-kat Here Eo is the initial enzyme concentration and ka=0.1 min¹. In an experiment with a partially pure enzyme preparation, 10 µg of total crude protein (containing enzyme) is added to a 1 mL reaction mixture containing 0.02 M ATP and incubated at 37C. After 12 hours, the reaction ends (i.e., t→∞) and the inorganic phosphate (P) concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: since [S]>>>Km, the reaction rate can be represented by: d (P) dt = K₂ [E]
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
Related questions
Question
![An enzyme ATPase has a molecular weight of 5 x 104 daltons, a KM value of 104 M and a
k2 value of k₂=104 molecules ATP/min molecule enzyme at 37 C. The reaction catalyzed
is the following
ATP → ADP + P
This can also be represented as:
E + S
k₁
k₁
E-S
K₂
E + P
Here S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation
kinetics are first order:
E = Ege-kat
Here Eo is the initial enzyme concentration and ka=0.1 min¹. In an experiment with a
partially pure enzyme preparation, 10 µg of total crude protein (containing enzyme) is
added to a 1 mL reaction mixture containing 0.02 M ATP and incubated at 37C. After 12
hours, the reaction ends (i.e., t→∞) and the inorganic phosphate (P) concentration is found
to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was
the enzyme?
Hint: since [S]>>>Km, the reaction rate can be represented by:
d(P)
dt
= K₂ [E]](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe80ef817-aa8b-4047-ac27-a3f534c77dd1%2Fa96b63eb-212b-40a4-9320-8601c68cc73b%2F6f5e54l_processed.png&w=3840&q=75)
Transcribed Image Text:An enzyme ATPase has a molecular weight of 5 x 104 daltons, a KM value of 104 M and a
k2 value of k₂=104 molecules ATP/min molecule enzyme at 37 C. The reaction catalyzed
is the following
ATP → ADP + P
This can also be represented as:
E + S
k₁
k₁
E-S
K₂
E + P
Here S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation
kinetics are first order:
E = Ege-kat
Here Eo is the initial enzyme concentration and ka=0.1 min¹. In an experiment with a
partially pure enzyme preparation, 10 µg of total crude protein (containing enzyme) is
added to a 1 mL reaction mixture containing 0.02 M ATP and incubated at 37C. After 12
hours, the reaction ends (i.e., t→∞) and the inorganic phosphate (P) concentration is found
to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was
the enzyme?
Hint: since [S]>>>Km, the reaction rate can be represented by:
d(P)
dt
= K₂ [E]
Expert Solution
![](/static/compass_v2/shared-icons/check-mark.png)
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 3 steps with 5 images
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
Recommended textbooks for you
![Introduction to Chemical Engineering Thermodynami…](https://www.bartleby.com/isbn_cover_images/9781259696527/9781259696527_smallCoverImage.gif)
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
![Elementary Principles of Chemical Processes, Bind…](https://www.bartleby.com/isbn_cover_images/9781118431221/9781118431221_smallCoverImage.gif)
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
![Elements of Chemical Reaction Engineering (5th Ed…](https://www.bartleby.com/isbn_cover_images/9780133887518/9780133887518_smallCoverImage.gif)
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
![Introduction to Chemical Engineering Thermodynami…](https://www.bartleby.com/isbn_cover_images/9781259696527/9781259696527_smallCoverImage.gif)
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
![Elementary Principles of Chemical Processes, Bind…](https://www.bartleby.com/isbn_cover_images/9781118431221/9781118431221_smallCoverImage.gif)
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
![Elements of Chemical Reaction Engineering (5th Ed…](https://www.bartleby.com/isbn_cover_images/9780133887518/9780133887518_smallCoverImage.gif)
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
![Process Dynamics and Control, 4e](https://www.bartleby.com/isbn_cover_images/9781119285915/9781119285915_smallCoverImage.gif)
![Industrial Plastics: Theory and Applications](https://www.bartleby.com/isbn_cover_images/9781285061238/9781285061238_smallCoverImage.gif)
Industrial Plastics: Theory and Applications
Chemical Engineering
ISBN:
9781285061238
Author:
Lokensgard, Erik
Publisher:
Delmar Cengage Learning
![Unit Operations of Chemical Engineering](https://www.bartleby.com/isbn_cover_images/9780072848236/9780072848236_smallCoverImage.gif)
Unit Operations of Chemical Engineering
Chemical Engineering
ISBN:
9780072848236
Author:
Warren McCabe, Julian C. Smith, Peter Harriott
Publisher:
McGraw-Hill Companies, The