For the Fluid Thioglycollate Medium, draw the five possible outcomes in the sections below and label accordingl (obligate aerobe, obligate anaerobe, facultative anaerobe, aerotolerant anaerobe, microaerophile)
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- Answer the following questions (not more than 5 sentences/question). Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.Define disinfection. Compare/contrast it with sterilization, antisepsis and bacteriostasis. Give the modes of action of the different antiseptics/disinfectants used in the activity (5% sodium hypochlorite (bleach), 0.05% sodium hypochlorite (bleach), 70% alcohol, 40% alcohol, Iodophor (Betadine), and Mouthwash)Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.
- Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungiAnswer the following questions: Therapeutic Classification of Sweet Orange Peel Tincture, USP Indication of Sweet Orange Peel Tincture, USPRead the following statements. Fill-in the possible effect of the procedure and give the rationale. (STERILITY TEST) The antimicrobial activity of the product tested was not eliminated. Effect: Rationale:
- Answer the following: which antimicrobial would be suitable for the control of the given bacterial cultures? Escherichia coli: MR-Staphylococcus aureus: Pseudomonas aeruginosa:Give the color of the potato mixed with Lugol's iodine solution in these situations before exposure in a water bath: after exposure in a water bath: after cooling from the water bath:General Instructions:Choose 1 bacteria/diseases and fill out the details. An example of an answered template is also provided for your reference. Causative Agent and Disease Profile for S. aureus Template and Example ITEM MSM PROFILE MICROBIAL PROFILE I MICROORGANISM/CAUSATIVE AGENT Staphylococcus aureus A GRAM REACTION (+) B OXYGEN REQUIREMENT Facultative Aerobes C SIZE 1.5 µm D SHAPE Cocci in clusters E HABITAT Normal flora of skin/anterior nares/pharynx F DISCOVERY G MICROSCOPIC IMAGE II DISEASE PROFILE Scalded skin syndrome A DISEASE/S Skin and Wound Infections Scalded Skin Syndrome Toxic Shock Syndrome Food Poisoning Pneumonia B SYMPTOMS OF THE DISEASE A high fever · Nausea and vomiting · A rash on your palms and soles that resembles a sunburn C INCUBATION PERIOD 2 and 4 hours (range 30 minutes to 8 hours) D MODE OF…
- a) Microbial products can be classified in three major categories which related to the microorganism growth curve. By giving examples of product based on the three major categories, sketch the microbial growth and product formation curves. Describe the phases involves in the growing of microorganism for the products formation. List all references with proper citations.Identify the method of sterilization/ chemical agent described At 15psi, the temperature reaches 121 degree Celsius requiring only 15 to 20 minutes to sterilize the material. The material to be sterilized is exposed to live steam for 30 minutes for 3 consecutive days.a physician prescribed an antibiotic to be mixed in white petrolatum to produce a 25% antibiotic preparation. The pyscian later changed the protocol to be only 12.5%. How much white petrolatum must be mixed with each 6-oz ointment container of 25% prepartation to make the new 12.5% preparation? Calculate answer in grams. Answer should be 180g