If your initial absorbance from undiluted crude dialysate is 4.50, determine the fold dilution needed to yield an absorbance of approximately 0.700. If you want to prepare 1.0 ml of diluted crude, what volume (mL) of crude dialysate must you add? Your Answer: Answer units

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If your initial absorbance from undiluted crude dialysate is 4.50, determine the fold
dilution needed to yield an absorbance of approximately 0.700. If you want to
prepare 1.0 ml of diluted crude, what volume (mL) of crude dialysate must you add?
Your Answer:
Answer
units
It is possible to estimate the molar extinction coefficient of a protein from
knowledge of its amino acid composition, as shown from your experiences with
EXPASY. From the molar extinction coefficient of tyrosine, tryptophan and cystine
(cysteine does not absorb appreciably at wavelengths >260 nm, while cystine does)
at a given wavelength, the extinction coefficient of the native protein in water can be
computed using the following equation:
e(Protein) = #(Tyr)*E(Tyr) + #(Trp)*E(Trp) + #(Cystine)*E(Cystine)
Where (if A280 measured in water): E (Tyr) = 1490, ɛ (Trp) = 5500, e (Cystine) = 125
Estimate the molar extinction coefficient of lysozyme using the above equation and
the amino acid composition data you found using the EXPASY bioinformatics server
during virtual 2, assuming a maximum number of cystine bonds.
) 37970
35920
36790
38940
Transcribed Image Text:If your initial absorbance from undiluted crude dialysate is 4.50, determine the fold dilution needed to yield an absorbance of approximately 0.700. If you want to prepare 1.0 ml of diluted crude, what volume (mL) of crude dialysate must you add? Your Answer: Answer units It is possible to estimate the molar extinction coefficient of a protein from knowledge of its amino acid composition, as shown from your experiences with EXPASY. From the molar extinction coefficient of tyrosine, tryptophan and cystine (cysteine does not absorb appreciably at wavelengths >260 nm, while cystine does) at a given wavelength, the extinction coefficient of the native protein in water can be computed using the following equation: e(Protein) = #(Tyr)*E(Tyr) + #(Trp)*E(Trp) + #(Cystine)*E(Cystine) Where (if A280 measured in water): E (Tyr) = 1490, ɛ (Trp) = 5500, e (Cystine) = 125 Estimate the molar extinction coefficient of lysozyme using the above equation and the amino acid composition data you found using the EXPASY bioinformatics server during virtual 2, assuming a maximum number of cystine bonds. ) 37970 35920 36790 38940
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