Propose a serial dilution that would give total dilution of 1/158000000 (six zeros) Each tube in your dilution series must have a volume of 50 ml or less
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- Propose a serial dilution that would give a dilution of 1/153 000 000. Each tube in your dilution series must have a volume of 50 ml or less.Complete the dilution series using a maximum amount of 20 mL of dilutent so that the dilution in the final tube is 10^-9Calculate the elution volume of blue dextran (blue), myoglobin (red), and Bromocresol purple (purple) in the gel filtration chromatography below with a flow rate of 2.5 mL/min. (Take note that the black colored test tubes represent colorless eluents)
- Draw a serial dilution that would give a dilution of 1/153 000 000. Each tube in your dilution series must have a volume of 50 ml or less.An infusion was ordered at 1.5 mcg/kg/min for a patient weighing 180lb. The available solution strength is 400 mg in 250 mL. What is the dosage in mcg/min?A medication order for a patient weighing 70 Kg is 0.75 mg of ampicillin per Kg BW to be added to 900 mL of 5% dextrose injection. The ampicillin injection is to be obtained in 2.5 mg/mL ampule. What is the total volume in mL of the IV admixture? Choose the best answer.
- A 158lb adult male diagnosed with endocarditis has an order for gentamicin 3mg/kg/day IV in 2 divided doses, in addition to vancomycin. The Gentamicin is available in 2 mL vials containing 40mg/mL. The facility protocol is to round the gentamicin dose to the nearest 10mg, then calculate the volume of solution to the nearest tenth mL. How many mL will administer per dose?The erythrocyte sedimentation rate (heaviness) test of a blood sample is often used as a diagnosis of immune disorders and some other diseases. Assuming that the red blood cells are about a sphere with a diameter of (approximately 5 um) and a density (g/ml 1.125), and the density and viscosity of the plasma in which the red blood cells are settled (Pa.s), (1025 kg/m3 10-3 x 1.5) On arrangement and acceleration m/s2 9.81 Calculate the erythrocyte sedimentation rate (settlement rate).For a gradient system with a gradient of 5-90% in 50 min, flow 2 mL/min, column 100 x 4.6 mm i.d., the first peak elutes at 20 min, and the last peak elutes at 50 min. Calculate k* for this system. 2. Can isocratic elution be used for this sample? If so, what is the range of k values to be expected. 3.Propose a way to shorten the gradient run time by eliminating the wait for the first peak to elute at 20 min. 4. For the original conditions of this question, change the conditions necessary to use a 150 x 4.6 mm column while maintaining the same k* value.
- KCI for injection contains 40 mEq in 20 mL. An order requires 15 mEq, how many mL are needed?How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? V1= 0.005mL B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?Calculate the final dilution in the following dilution series: a. 1 mL in 9 mL dilution & 100uL spread onto the plate b. 1 mL in 9 mL dilution followed by a 100ul in 900ul dilution & 100uL spread onto the plate (note bacteria are counted on plates as cfu/mL)