QUESTION 26 In hydrophobic interaction chromatography (HIC), which separates proteins based on their hydrophobicity, the bound proteins are eluted by proteins are eluted by Choose a number from the choices shown below. In the case of ion-exchange chromatography, the bound For exclusion chromatography we use 1. increasing NaCl concentration of the buffer. 2. decreasing NaClI concentration of the buffer. 3. increasing acetonitrile concentration of the buffer. 4. decreasing acetonitrile concentration of the buffer. 5. increasing pH of the buffer. 6. decreasing pH of the buffer. 7. none of the above
QUESTION 26 In hydrophobic interaction chromatography (HIC), which separates proteins based on their hydrophobicity, the bound proteins are eluted by proteins are eluted by Choose a number from the choices shown below. In the case of ion-exchange chromatography, the bound For exclusion chromatography we use 1. increasing NaCl concentration of the buffer. 2. decreasing NaClI concentration of the buffer. 3. increasing acetonitrile concentration of the buffer. 4. decreasing acetonitrile concentration of the buffer. 5. increasing pH of the buffer. 6. decreasing pH of the buffer. 7. none of the above
Chapter8: Polyfunctional Acids And Bases
Section: Chapter Questions
Problem 14P
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