Question 5 Listen In gene therapy trials such as Molly's, a functional copy of the gene is put into the cells. Based on this fact, what would you expect for such gene therapy? (This question is based on the video about gene therapy) a) It would work best with recessive conditions b) It would work best with dominant conditions c) It would work equally well with recessive and dominant conditions
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- QUESTION 7 After you graduate, you are working at a laboratory that identifies a novel protein called Leprechaun. To determine in which organelles Leprechaun is found, you decide to express the Leprechaun gene in HeLa cells using a plasmid. Since you have generated an antibody that recognizes an epitope within the Leprechaun protein, which technique will you use to identify its localization within the HeLa cells? OA. SDS-PAGE O B. Immunocytochemistry O C. Western blot OD. Transmission electron microscopy (TEM) OE. Immunohistochemistry QUESTION 8 Which of the following statements regarding electron microscopy is TRUE? OA. White light is utilized to illuminate the sample for imaging in all types of electron microscopy. OB. Electrons pass through the object being examined in transmission electron microscopy (TEM). OC. In scanning electron microscopy (SEM) electrons, electrons bounce off the surface of the sample. OD. Electron microscopy can be used to view structures as small as 2 nm in…Question 16 Why can't SNPS be detected by PCR and Gel Electrophoresis? O Because Gel Electrophoresis detects size differences in DNA and SNPS do not change the size of the DNA strand. O Because SNPS cause deletions so large that they are beyond the limits of this technique to detect. O Because SNPS affect proteins and PCR only works on DNA. O Because SNPS are too complicated to detect with this technology.Question 35 Based on the restriction enzyme specificities given below, which will generate blunt ends? Restriction Enzyme Specificities: GAATTC. Hpal .GTTAAC.. ...СТТААG... PstI .CTGCAG... .GĄCGTC. ECORI .CAAȚTG... Hind II .. AAGCTT... Bam HI...GGATCC.. .CCTAGG.. ТTCGAA... A) Hindlll B) Pstl BamHI D) Hраl E) EcoRI
- [ Choose ] [Choose ) in vitro mutagenesis CRISPR-Cas9 system SNP (single nucleotide polymorphism) RNA interference (RNAI) >QUESTION 1 You want to perform PCR on the CDNA of the spike gene from a SARS CoV-2 sample so that you can sequence it. Based on the sequence below, which of the following primer pairs would probably work for PCR of this gene? Spike gene Sequence: 5' ATGTTTATTTTCTTATTATTTTTTACTCTCACTAGTGGTAGTGACCTTGACCGGTGCACCACTTTTGATG ATGTTCAAGCTCCTAATTACACTCAACATACTTCATCTATGAGGGGGGTT TACTATCCTGATGAAATTTT. .. (it's really long so didn't post the whole thing.).TCTTGCTTTGTTGCATGACTAGTTGTTGCAGTTGCCTCAAGGGTGCATGCTCTTGTGGTTCTTGCTGCAA GTTTG ATGAGGATGACTCTGAGCCAGTTCTCAAGGGTGTCAAATTACATTACACATAA 3' Forward primer: 5' - CTC TCA CTA GTG GTA GTG ACC - 3' (Tm = 60.5 °C in a standard qPCR mix) Reverse Primer: 5' GGG TGT CAA ATT ACA TTA CAC ATA - 3' (Tm= 59.6 °C in a standard QPCR mix) Forward Primer: 5'- ATG TTT ATT TTC TTA TTA TT -3' (Tm=D 47.2 °C in a standard qPCR mix) Reverse Primer: 5'- GCA AGA ACC ACA AGA GCA TGC ACC -3' (Tm= 68 °C in a standard qPCR mix) Forward primer: 5' - CTC TCA CTA GTG GTA GTG ACC -3'…Question 14 The term "gene expression" refers to the O process by which genetic information is transformed nucleic acid information into a functional protein. the fact that each individual of a species has a unique set of genes. the fact that individuals of the same species have different phenotypes. flow of information from parent to offspring. Question 15 An operon consists of the coding sequences of a cluster of genes regulatory gene, coding sequences of a cluster of genes, an operator, and a promoter. a promoter, an operator and the coding region of a gene. a promoter and the coding region of a gene.
- Question 7 Albinism is caused by an autosomal recessive allele that interferes with skin pigmentation in mammals. Two normally pigmented parents already have an albino boy. They plan to have 2 more children. a) What is the probability that their next two children will be normally pigmented? Show your work. b) What is the probability that their next two children will both have albinism? Show your work. c) What is the probability that, when they have 2 more children, they will end up with a family including a total of 2 albino children and one normal? Show your work. d) What is the probability that, when they have two more children, the next child (i.e. the second-born child) will be normal, followed by a child with albinism? Show your work. e) Explain why the answers to part (c) and part (d) are calculated differently.Question 9B If Mendel had done his experiments using flies instead of peas, what factors would he have to have dealt with that weren't factors with the system that he used? Would he have been likely to have come to the same conclusions using flies instead of peas? Edit View Insert Format Tools TableQuestion:- 1. Brassica oleracea is the wild plant from which brussels sprouts, cabbage, broccoli, and cauliflower were domesticated. Why is it important to keep many diverse seeds from the forb B. oleracea across its range? A. If our B. oleracea-derived crops are driven to extinction, we can re-domesticate them from the wild plant. B. Our crop Brassicas are much more genetically diverse than B. oleracea because of extensive breeding programs, so we need to conserve as much of B. oleracea's diversity as possible. C. The budding pattern in Brassica may be further modified to produce more productive crop plants. D. B. oleracea may have gene variants that can be incorporated into our domesticated plants to increase their environmental range or tolerance to pests. E. We may be able to domesticate another crop plant from B. oleracea.
- QUESTION 6 To verify the is indeed inside your plasmid, you'd like to do a colony PCR. But you need primers for your reaction. Which of the following primer pairs would probably work for verifying your insert is actually present in the plasmid? 5' ATGTTTATTTTCTTATTATTTTTTACTCTCACTAGTGGTAGTGACCTTGACCGGTGCACCACTTTTGATG ATGTTCAAGCTCCTAATTACACTCAACATACTTCATCTATGAGGGGGG TTTACTATCCTGATGAAATTTT (Very long, but a bunch of nucleotides her e).... TCTTGCTTTGTTGCATGACTAGTTGTTGCAGTTGCCTCAAGGGTGCATGCTCTTGTGGTTCTTGCTGCAA GTTTGATGAGGATGACTC TGAGCCAGTTCTCAAGGGTGTCAAATTACATTACACATAA 3' Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm= 59.8 O A. Reverse: 5' CAA ATT ACA TTA CAC ATA A 3' Tm= 47.4 Forward: 5' ATG TTT ATT TTC TTA TTA TTT 3' Tm= 47.1 C O B. Reverse: 5' TAT GTG TAA TGT AAT TTG ACA CCC 3' Tm3 58.4 Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm3 59.8 OC. Reverse: 5' GTG TAA TGT AAT TTG ACA CCC TTG 3' Tm: 59.4 Forward: 5' GGT CAC TAC CAC TAG TGA GAG 3' 59.4 C O D. Reverse: 5' GTG TAA TGT AAT TTG ACA CCC TTG…QUESTION 45 Gene edits can be made to Eukaryotic DNA by using a complex composed of: Progenitor cell and 4 master regulatory genes Cyclic AMP and CRP protein Ubiquitin protein and CRISPR Guide RNA and Cas 9 protein Histone proteins and methyl groups, QUESTION 46 Click Saye andQuestion -The FDA has authorized the use of direct-to-consumer testing for three mutations in BRCA genes that elevate cancer risk, but cautions that a negative result does not rule out increased cancer risk. How can this be true? A. It is impossible to trust companies that are selling genetic tests. B. They have a conflict of interest, and so the tests should be used for entertainment value only C. These tests are not highly accurate, and false negatives are possible. Individuals with a family history should have a negative result confirmed with a different test to be sure they are truly at low risk of developing cancer There are more ways to get cancer than a mutation in the BRCA gene. D. The test only detects three out of more than 1,000 known BRCA mutations. This means a negative result does not rule out the possibility that an individual carries other BRCA mutations that increase cancer risk..