R Amp 8,000 Xhol 0 (or 12,000) PUC 18 12,000 bp 2,000 insert 4,000 EcoRI alone HindIll alone Xhol alone EcoRI+ Hindlll EcoRI + Xhol HindIll + Xhol 12,000 bp 12,000 bp 12,000 bp 3,000 and 9,000 bp 2,000 and 10,000 bp 5,000 and 7,000 bp You have cloned a DNA fragment into the plasmid pUC18 shown above. The pUC vector (solid part) contains the ampicillin (AmpR) resistance gene, as well as one recognition site for the restriction enzyme Xhol and one recognition site for EcoRI, but no recognition sites for Hindill. The inserted DNA fragment is indicated by the striped bar. You perform several restriction digests of the recombinant plasmid, using each enzyme alone or in combination with another enzyme. The sizes of the resulting DNA fragments are shown in the table 30) At what position number on the plasmid is the HindIll site? a) 2,000 bp b) 3,000 bp c) 6,000 bp d) 8,000 bp

R Amp 8,000 Xhol 0 (or 12,000) PUC 18 12,000 bp 2,000 insert 4,000 EcoRI alone HindIll alone Xhol alone EcoRI+ Hindlll EcoRI + Xhol HindIll + Xhol 12,000 bp 12,000 bp 12,000 bp 3,000 and 9,000 bp 2,000 and 10,000 bp 5,000 and 7,000 bp You have cloned a DNA fragment into the plasmid pUC18 shown above. The pUC vector (solid part) contains the ampicillin (AmpR) resistance gene, as well as one recognition site for the restriction enzyme Xhol and one recognition site for EcoRI, but no recognition sites for Hindill. The inserted DNA fragment is indicated by the striped bar. You perform several restriction digests of the recombinant plasmid, using each enzyme alone or in combination with another enzyme. The sizes of the resulting DNA fragments are shown in the table 30) At what position number on the plasmid is the HindIll site? a) 2,000 bp b) 3,000 bp c) 6,000 bp d) 8,000 bp

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter17: Metabolism: An Overview

Section: Chapter Questions

Problem 13P: What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome...

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EcoRI alone wwwwwwwww HindIll alone Bam HI alone EcoRI+Hindill EcoRI + BamHI HindIll+ BamHI 15,000 bp 15,000 bp 15,000 bp 7,000 and 8,000 bp 4,000 and 11,000 bp 4,000 and 11,000 bp Amp PUKY plasmid 15,000 bp 2,000 EcoRI insert 7,000 You have cloned a DNA fragment into the plasmid pUKY shown above. The insert contains one EcoRI site but no recognition sites for Hind!!! or BamHI. The position of the EcoRI site is indicated on the figure below. The extent of the inserted DNA fragment is indicated by the striped bar. You perform several restriction digests of the recombinant DNA, using each enzyme alone or in combination with another enzyme. The sizes of the resulting restriction fragments are shown in the table. If you digested the recombinant plasmid with BamHI, EcoRI, and HindlII, and then separated the restriction fragments by gel electrophoresis, how many bands should you see on the gel?
Numbering the fragments left by cutting the DNA with BAM HI from left to right, which fragment will travel the furthest? BAM HI: GGATCC CCTAGG AATCGGATCCATTTGGACTAAAGGACCCGGATTGGATCCAGGGCCTTTAGTACC TTAGCCTAGGTAAACCTGATTTCCTGGGCCTAACCTAGGTCCCGGAAATCATGG O 3 O 2 4. 1.
Fig 3.16 EcoRI SacI KpnI |ampR Aval Xmal Smal lacZ BamHI Xbal SalI Accl HincII PstI Sphl HindIII Bam H1 Bam H1 Bam H1 1 2 3 4 The ends of the double-stranded DNA fragment above are blunt ends. The directionality of genes contained within the fragment is from left to right (arrow). After digestion by BamH1, which fragment can be inserted into the vector cut with BamH1 and Sma 1 maintaining the same directionality as the lacz promoter (green segment with arrow on vector)? 3 O 4 O1 2
Replicate the DNA strand AAGGCTAACGGCATTTAACCC. Transcribe the DNA strand AAGGCTAACGGCATTTAACCC. Translate your answer to #16 using the table below. Second letter A G UGU cys UGC UUU PheUCU UCC UCA UCG UAU1 UUC UUA UAC J Tyr Ser UAA Stop UGA Stop A UAG Stop UGG Trp G UUGLEU CAUTHIS CÁC CUU CUC CUA CUG CCU] CCC CCA CCG CGU] CGC Arg Leu Pro CAA GIn CGA CGG. CAGS ACU ACC ACA AAC FAsn AAA AGU Ser AGC AGA LArg Lys AGGJ AUU AAU AUC le A AUA The AUG Mer ACG AAGJ GAU ASP GACJ GUU GCU GCC GCA GCG GGU GGC Gly GUC Val GUA Ala GAAG Glu GAGJ GGA GUG GGG Third letter DUAG JCAG DUAG C. First letter
For the chromatogram below, what is the sequence of the template DNA from base 115 to 125? CTGTGTGAAA TTGT TA T C CGC T CACA A T TCCACA CA A CATACGAG CCGGAAG CA T AA 110 120 130 140 150 160 СТТТААСАAТА ТАTTCAATTТС ATAACAATTTC GAAATTGTTAT
Second Base Analyze the following DNA sequences: A UUU UCU UAU UGU Phe Tyr UUC UCC UAC UGC Original: AGAGAGAGAGAGAGAGAG Ser UUA UCA UAA Stop UGA Stop Leu UUG UCG UAG Stop UGG Trp CUU CCU CAU CGU His Mutated: AGAAGAGAGATCGAGAGA CUC CC CAC CGC Leu Pro Arg CỦA CCA CAA CGA Gin CUG CCG CAG CGG AUU ACU AAU AGU Ser Asn What amino acid sequence will be translated based on the AUC Ile ACC AAC AGC The mutated DNA? Hint* must transcribe and translate the AUA ACA AGA AAA arg Lys Met or Start AUG ACG AAG AGG mutated strand to find the answer. GUU GCU GAU GGU Asp GUC GCC GAC GGC Val Ala Gly G GUA GCA GAA GGA Glu GUG GCG GAG GG First Base Third Base
There are numerous methods for sequencing DNA, including classical Sanger sequencing, automated Sanger sequencing, and next-generation sequencing technologies, including Illumina technology. Match the modified cytidine nucleotide triphosphates (NTPs) with DNA sequencing methods that utilize them. Classical Sanger sequencing HO-P=O O HO-P=O O HO-P=O O Automated Sanger sequencing -NH₂ CH3 H₂CN ° ? ?N HOOOO OH OH OH NH CH₂ Next-generation DNA sequencing (Illumina) CH₂ NH₂ Answer Bank
In a genome project, the following genomic DNA sequences were obtained. Assemble the sequences into a contig. Using the assembled sequence, perform a BLASTn search. Does the search produce sequences similar to your assembled sequence? 5’ TCGGGGTCCTGGGATCTCATCACTGCAGCGC 3’ 5’ACTGCAGCGCTTTCCCAGCGGGCGGTGGTAC 3’ 5’GGGCGGTGGTACTCGGGAAGTCAGGAGTGTT 3’ 5’AGGAGTGTTTAAAACCTGGGGACTGGTTTTG 3’ 5’TGGTTTTGGGGGCGCTGAAGGCAGCGCAGGA 3’
Transcribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’
What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome c. Proteome d. Metabolome e. Fluxome
Transcribe and translate the following DNA sequence (nontemplate strand): 5'- ATGGCCGGTTATTAAGCA-3'
During proofreading, which of the following enzymes reads the DNA? primase topoisomerase DNA pol helicase