The table below shows the purification of liver lactate dehydrogenase. Purification Table of liver LDH Step Volume DEAE-Sephadex 5'AMP-Sepharose (mL) Crude Extract 200 Ammonium Sulfate 100 112 40 LDH activity (Units/mL) (mg/mL) [Protein] 47.0 50.00 90.0 63.00 51.0 1.50 137.0/ 0.4 Calculate the LDH yield for the ammonium sulfate step. Provide answer to one decimal only.

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter21: Heparin Infusion Calculations
Section: Chapter Questions
Problem 1.3P
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The table below shows the purification of liver
lactate dehydrogenase.
Purification Table of liver
LDH
Step
Crude Extract
Ammonium Sulfate
DEAE-Sephadex
5'AMP-Sepharose
Volume
(mL)
200
100
112
40
LDH
activity
(Units/mL) (mg/mL)
[Protein]
47.0
50.00
90.0
63.00
51.0
1.50
137.0/ 0.4
Calculate the LDH yield for the ammonium sulfate
step. Provide answer to one decimal only.
Show Transcribed Text
this is biochemistry and the topic is protein
purification
could you pleaae show me the full workings
even the workings for the comversions
Transcribed Image Text:The table below shows the purification of liver lactate dehydrogenase. Purification Table of liver LDH Step Crude Extract Ammonium Sulfate DEAE-Sephadex 5'AMP-Sepharose Volume (mL) 200 100 112 40 LDH activity (Units/mL) (mg/mL) [Protein] 47.0 50.00 90.0 63.00 51.0 1.50 137.0/ 0.4 Calculate the LDH yield for the ammonium sulfate step. Provide answer to one decimal only. Show Transcribed Text this is biochemistry and the topic is protein purification could you pleaae show me the full workings even the workings for the comversions
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