Using absorbance at 234 nm, 280 nm, and 260 nm, you calculated the percent of proteins present in your DNA. The percent of proteins present when calculated using absorbance at 260 nm and 280 nm was much larger than the percent of proteins present calculated using absorbance at 260 nm and 234 nm. This indicated that: a. Peptides present in your DNA preparation contain a large number of amino acids, tyrosine, phenylalanine, and tryptophan. 5. Peptides present in your DNA preparation contain a small number of amino acids tyrosine, phenylalanine, and tryptophan. C. Peptides present in your DNA preparation do not contain amino acids tyrosine, phenylalanine, and tryptophan. d. Your DNA preparation is not contaminated with proteins. e. All of the above. f. None of the above.

Organic Chemistry
8th Edition
ISBN:9781305580350
Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Chapter20: Dienes, Conjugated Systems, And Pericyclic Reactions
Section: Chapter Questions
Problem 20.26P
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Propose an appropriate intermediate for the following reaction

Using absorbance at 234 nm, 280 nm, and 260 nm, you calculated the percent of
proteins present in your DNA. The percent of proteins present when calculated
using absorbance at 260 nm and 280 nm was much larger than the percent of
proteins present calculated using absorbance at 260 nm and 234 nm. This
indicated that:
a. Peptides present in your DNA preparation contain a large number of amino
acids, tyrosine, phenylalanine, and tryptophan.
b. Peptides present in your DNA preparation contain a small number of amino
acids tyrosine, phenylalanine, and tryptophan.
c. Peptides present in your DNA preparation do not contain amino acids
tyrosine, phenylalanine, and tryptophan.
d. Your DNA preparation is not contaminated with proteins.
e.
All of the above.
f. None of the above.
Transcribed Image Text:Using absorbance at 234 nm, 280 nm, and 260 nm, you calculated the percent of proteins present in your DNA. The percent of proteins present when calculated using absorbance at 260 nm and 280 nm was much larger than the percent of proteins present calculated using absorbance at 260 nm and 234 nm. This indicated that: a. Peptides present in your DNA preparation contain a large number of amino acids, tyrosine, phenylalanine, and tryptophan. b. Peptides present in your DNA preparation contain a small number of amino acids tyrosine, phenylalanine, and tryptophan. c. Peptides present in your DNA preparation do not contain amino acids tyrosine, phenylalanine, and tryptophan. d. Your DNA preparation is not contaminated with proteins. e. All of the above. f. None of the above.
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UV-visible absorption spectroscopy for proteins and DNA

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