What are the basic differences between the three plating methods? Fill up the following table. Streak Pour Spread Equipment/ Materials used in immobilizing and separating cells Purpose(s) (isolation, enumeration, both) Type of colonies (surface, subsurface, both) Advantage Disadvantage
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What are the basic differences between the three plating methods? Fill up the following table.
Streak | Pour | Spread | |
Equipment/ Materials used in immobilizing and separating cells |
|||
Purpose(s)
(isolation, enumeration, both) |
|||
Type of colonies (surface, subsurface, both) |
|||
Advantage | |||
Disadvantage |
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- Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observed(1) why can't we say "sterile" technique (2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities. (3) You are asked to develop a method of transfer an unknown organism from a liquid broth to a solid petri dish.list each step that you would have to take .be specificWhat are the advantages and disadvantages of using the Wet Mount technique? What are the advantages and disadvantages of using the Hanging drop technique? What are the advantages and disadvantages of using the Slide Culture technique? pls elaborate each, thank you
- Determine what percentage of the culture was living (viable) and what percentage was dead (mortality). Plates Plate Dilution Volume plated No.of colonies Avg No Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 1 10^-3 10ul R1=130,R2= 110,R3=210 150 150mL 1.50*10^6 Volume of cells(mL) Volume of diluent(mL) Total dilution(D) Hemocytometer count Avg cells in 1 mm^2 area Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 4.3 0.5 0.1 grid 1= 171 , grid2 = 185 178 1.78*10^5 1.78*10^6Select all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cells- What is a pure culture? - Describe the procedure for making a streak plate from a clinical specimen (like a urine culture). - What is the desired outcome (result) of a streak plate? - Clinically, why is it important to make a pure culture from a clinical specimen? What do you do next in order to treat the patient?
- What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handleYou are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution schemeSelect all that apply to a direct simple stain: 1. the dye is acidic or negatively charged and stains the background 2. uses multiple dyes in the procedure 3. cells will never be distorted or shrunken 4. uses only 1 dye in the procedure 5. does involve a washing step 6. can be used to determine cell morphology, size, and arrangement. 7. does involve fixing 8. the dye is basic or positively charged and stains the bacteria 9. cells may be distorted or shrunken 10. does not involve a washing step 11. does not involve fixing
- There are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2What are the equipments/ materials used in imoobilizing and separating cells when using: spread plate, streak plate, and pour plate method?You have identified two colony types A and B on the streak plate. Now briefly describe (in 3-4 sentences) the process of how you identified the cellular morphology/arrangement of each isolate. Again, assume you have all the necessary equipment and materials at your disposal. Be concise and thorough, not verbose; e.g., refer to the Gram stain, but describing the details of each step is not necessary.