What does RNAi do? O a. O b. O C. It binds to DNA double helix and cleaves DNA. It inhibits transcription. It degrades proteins. O d. It inhibits DNA replication. O e. It binds to mRNA and cleaves mRNA.
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- 8) Which of these describes the function of RNA polymerase? A. Amplifies the “message" by making multiple copies of an mRNA molecule after it has been transcribed from DNA B. Converts a protein sequence to mRNAThe gene for the primase enzyme is mutated and is nonfunctional. What is the result of such a mutation? Select a statements that are true O a. Leading strand synthesis is inhibited O b. The leading strand includes RNA, but the lagging strand does not Oc Leading strand fragments are not joined O d. Lagging strand synthesis is inhibited1. Create a DNA sequence with eighteen nucleotides. Indicate its 3’ on the left and 5’ on the right since that’s the template strand you will need in the next question to transcribe the mRNA. 2. Transcribe the DNA sequence above and separate the triplets into codons. Indicate 5’ and 3’ in the correct location on the strand. (Don’t worry about splicing- assume that the pre- mRNA is the same as the mature mRNA sequence) 3. Look at the genetic code, and indicate which amino acid is coded for by the codons in the above mRNA. 4. ANSWER BELOW QUESTIONS: A. First write the original DNA strand. Indicate where the substitution was by either circling it or writing it in a different color. Then write the mutated DNA sequence with the point mutation (aka substitution) wherever you choose for it to be. Again, circle it or write it in a different color. Do the same for the transcribed mRNA. Repeat the directions for 2 and 3 for this new DNA stand. (i.e., include the mRNA and translated protein…
- Which of the following is incorrect? a. Amino acids may have one or more triplet codes or codons b. Translation refers to the synthesis of RNA DNA replication is possible due to the complementarity of the two strands of DNA Od. A mutated gene can cause disease O c.1. One strand of DNA has the base sequence: CGATTGGCAGTCA T. Determine the sequence of bases in the complementary strand of MRNA that would form next to this DNA strand. a. Write the sequence of bases in the complementary MRNA strand below.RNA primers are necessary in DNA synthesis because OA. primers mark the sites where helicase has to unwind the DNA. OB. the DNA polymerase enzyme can catalyze the addition of deoxyribonucleotides only onto the 3' (-OH) end of an existing strand. O C. RNA primase is the first enzyme in the replication complex. D. they are the source of nucleotides for DNA polymerases. 00 O E. DNA polymerase is unable to initiate replication without an origin. 5 p
- At the beginning of this century, there was a general announcement regarding the sequencing of the human genome and the genomes of many other multicellular eukaryotes. Many people were surprised that the number of non-coding sequences was much larger than they had expected. Which of the following types of DNA make up the rest of the human genome?RNA molecules differ from DNA molecules in that they * a. are single stranded rather double stranded b. contain five different bases rather than four c. contain a greater number of nucleotide units d. no correct response Which of the following is definitely an incorrect codon-amino acid pairing? * UUU-UUC and Phe-Phe ACU-AAU and Thr-Asn GCC-GCC and Ala-Gly ACU-GUA and Val-Pro Which of the following statements concerning codons is incorrect? * a. they are found within mRNA molecules b. they specify base sequence in terms of four-nucleotide units c. there are 64 of them d. no correct responseNucleotides play a variety of roles in the cell. Give an example of a nucleotide that acts in each of the following roles or processes.a. Second messengerb. Phosphoryl-group transferc. Activation of carbohydratesd. Activation of acetyl groupse. Transfer of electronsf. DNA sequencingg. Chemotherapyh. Allosteric effector
- Which of the following statements concerning translation is NOT correct? O b. O c. Polypeptide chain is extended from the C- terminus to the N-terminus. O d. The first codon of protein synthesis is AUG. Prokaryotic transcription and translation are coupled (i.e. they occur almost at the same time and at the same place). O e. Protein synthesis takes place in ribosome. During protein synthesis, the mRNA is read from 5' to 3' direction.What is an anticodon, and where are they found? O a. Anticodons are complementary to codons, and are found in DNA genes O b. Anticodons are complementary to codons, and are embedded in tRNA molecules O c. Anticodons are antiparallel to codons ("backwards codons") and are found on the coding strand of the DNA gene O d. Anticodons encode amino acids and are found in mRNA moleculesTranscription is a process in which MRNA is synthesized from DNA mRNAis pro duced from only one strand The strand while the other strand, which does not play any strand The statement given ab ove is complete d by the information in DNA strand which trans cribes RNA is called the role in transcription is known as the 11 row. Row 11 A. sense antisense B antisense s ense sense sense antisense antisense
