Why are quantitative absorbance measurements made at lambda max?
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Why are quantitative absorbance measurements made at lambda max?
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- Assume that you have an “X” solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. Prepare a standard curve by using the absorbance of known concentrations (Hint: you can learn how to prepare a standard curve by using Excell from youtube). Calculate the concentration of unknown.The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.What is happening in this trace? What do the peaks represent? Why is absorbance measured at 230 nm?
- How do you measure the amount of µmoles of a substance from absorbance? If the absorbance is 0.019, the extinction coefficient is 0.0106(µmol/mL)-1 cm-1, path length is 1cm, and the total volume was 4 mL.Guanosine (C10H13N5O5) in solution has a maximum absorbance at a wavelength of 275 nm. The molar extinction co-efficient at this wavelength is 84M−1cm−1and the path length is 24.7 cm. Through the use of a spectrophotometer, it is found that the that A275= 1.48. What is the concentration of the guanosine solution in grams/litre? Molecular weights (g/mol): C-12, H-1, N-14, O-16 Select one: A. 0.201869 g/L B. 0.435188 g/L C. 0.059919 g/L D. 0.294046 g/L E. 0.000713 g/LA calibration curve for the absorbance of an unknown is shown below. What would be the concentration of a sample with absorbance of 0.45. Give the answer with the correct unit.
- Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement or during the transfer to the spectrophotometer?Discuss the types of interferences in atomic absorption spectrophotometry.explain the results of this experiment Table 1 Absorption Spectrum of Cobalt Chloride Using a Spectrophotometer Wavelength Absorbance 400 410 420 430 440 450 460 370 480 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 0.036 0.044 0.063 0.095 0.147 0.215 0.284 0.329 0.362 0.402 0.450 0.479 0.464 0.398 0.314 0.216 0.142 0.087 0.054 0.041 0.036 0.036 0.034 0.028 0.030 0.023 0.023 0.023 0.014 0.014 0.011 Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.042 3 0.018 0.92 4 0.027 0.139 5 6 0.036 0.045 0.190 0.236 7 (Unknown) 0.151
- This is a question regarding spectrophotometry. Scenario: 0.1 mL of the unknown and 0.9 mL of distilled water are mixed in a cuvette and the absorbance is measured at 280 nm. Given the absorbance, molecular mass and the extinction coefficient of the unknown are 0.253, 16950 g and 13940 M-1cm-1 respectively, and the path length is 1 cm, how can I find the concentration of the unknown in mg/mL by Beer-Lambert Law (NOT molarity M)?The electronic excitation method in fluorescence and absorption spectroscopy is the same. What are some of the variations between using a fluorimeter to perform an excitation scan and an absorption scan? Do you think the two spectra should be similar? Describe. More info attached like protocol if neededDetermine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis.