Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 11.3, Problem 1CC
If this experiment was conducted for four rounds of
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In the Meselson-Stahl experiment on DNA replication, what fraction of the DNA was composed of one light strand and one heavy strand ("hybrid") after one generation of growth in medium containing 14N? After two generations of growth in a medium containing 14N? What fraction of hybrid DNA is expected after n generations of growth in a medium containing 14N?
The chromosome of E. coli contains 4.6 million bp. How long will it take to replicate its DNA? Assuming that DNA polymerase III is the primary enzyme involved and that it can actively proofread during DNA synthesis, how many base pair mistakes will be made in one round of DNA replication in a bacterial population containing 1000 bacteria?
What result would Meselson and Stahl have obtained if the replication of DNA were conservative (i.e., the parental double helix stayed together)? Give the expected distribution of DNA molecules after 1.0 and 2.0 generations for conservative replication.
Chapter 11 Solutions
Biology
Ch. 11.1 - Prob. 1CSCh. 11.1 - Prob. 1EQCh. 11.1 - Prob. 2EQCh. 11.1 - CoreSKILL In the experiment of Avery, MacLeod,...Ch. 11.2 - Prob. 1CCCh. 11.2 - Prob. 2CCCh. 11.2 - Core Skill: Modeling The goal of this modeling...Ch. 11.2 - Prob. 2CSCh. 11.3 - If this experiment was conducted for four rounds...Ch. 11.4 - Molecular Mechanism of DNA Replication Concept...
Ch. 11.4 - Prob. 2CCCh. 11.4 - Prob. 3CCCh. 11.4 - Prob. 4CCCh. 11.5 - Prob. 1CCCh. 11.5 - Prob. 1CSCh. 11 - Why did researchers initially believe that the...Ch. 11 - Prob. 2TYCh. 11 - Which of the following equations is accurate...Ch. 11 - Prob. 4TYCh. 11 - Which of the following statements about the...Ch. 11 - Meselson and Stahl were able to demonstrate...Ch. 11 - During replication of a DNA molecule, the daughter...Ch. 11 - Prob. 8TYCh. 11 - A nucleosome is a. a dark-staining body composed...Ch. 11 - The conversion of euchromatin into heterochromatin...Ch. 11 - What are the four key criteria that the genetic...Ch. 11 - A double-stranded DNA molecule contains 560...Ch. 11 - Prob. 3CQCh. 11 - Prob. 1COQCh. 11 - CoreSKILL How might you provide evidence that DNA...
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- You decide to repeat the Meselson-Stahl experiment, except this time you plan to grow the E. coli cells on light 14N medium for many generations and then transfer them to heavy 15N medium and allow them to grow for 2 additional generations (2 rounds of DNA replication). If the conservative model of DNA replication was correct, what is the expected distribution of DNA in the density gradient after two rounds of replication? Multiple Choice One band of intermediate density. One band of light density. One band of heavy density. One band of light density and one band of heavy density. One band of light density and one band of intermediate density.arrow_forwardApproximately how many high-energy bonds does DNA polymerase use to replicate a bacterial chromosome (ignoring helicase and other enzymes associated with the replication fork)? compared with its own dry weight of 10–12 g, how much glucose does a single bacterium need to provide enough energy to copy its DNA once?arrow_forwardYou decide to repeat the Meselson-Stahl experiment, except this time you plan to grow the E. coli cells on light 14N medium for many generations and then transfer them to heavy 15N medium and allow them to grow for 2 additional generations (2 rounds of DNA replication). If the conservative model of DNA replication was correct, what is the expected distribution of DNA in the density gradient after two rounds of replication?arrow_forward
- The Meselson-Stahl experiment provided strong evidence that DNA replication was conservative, by alternately growing bacteria in medium with heavy 15N and light 14N. If DNA replication were dispersive, what result would Meselson and Stahl have observed after the first round of DNA replication in light nitrogen? Group of answer choices Two bands, one at the location for pure 15N and one at the location for pure 14N. One band, located half way between the locations for pure 15N and pure 14N. Two bands, one at the location for pure 15N and one located halfway between the locations for pure 15N and pure 14N. None of these Three bands, one at the location for pure 15N, one at the location for pure 14N, and one at a location halfway between.arrow_forwardWhy were Meselson and Stahl unable to distinguish between the dispersive and the semiconservative models following only one round of DNA replication?arrow_forwardIf we are given this segment of DNA: TTGGHTGUTGG HHUUTHUGHUU Let’s suppose this DNA was treated with nitrous acid. The nitrous acid was then removed, and the DNA replicated for two generations. What would be the sequences of the DNA products after the DNA had replicated two times? (note Hypoxanthine pairs with cytosine) and so there would be four sets?arrow_forward
- Based on the electrophoresis experiment, 0.7% agarose gel concentration was used. If the gel concentration increased to 1%, what would happen to the DNA migration? Explain briefly.arrow_forwardThe E. coli chromosome is 1.28 mm long. Under optimal conditions the chromosome is replicated in 40 minutes. (a) What is the distance traversed by one replication fork in 1 minute? (b) If replicating DNA is in the B form (10.4 base pairs per turn), how many nucleotides are incorporated in 1 minute in one replica- tion fork? (c) If cultured human cells (such as Hela cells) replicate 1.2 m of DNA during a 5-hour S phase and at a rate of fork movement one- tenth of that seen in E. coli, how many origins of replication must the cells contain? (d) What is the average distance, in kilobase pairs, between these origins?arrow_forwardTo prepare a sample for electrophoresis, samples of the DNA being investigated (a) are put into each of four tubes and induced to replicate (b). Also, into the first tube, an adenine terminator was added in addition to all the other nucleotides. As the complementary strand was being constructed, the terminators were occasionally incorporated wherever an adenine nucleotide was used. This random incorporation resulted in all possible lengths of DNA pieces that had an adenine on the end (c). The same process was conducted in the other tubes with thymine, guanine and cytosine terminators; one treatment for each of the four lanes in the gel. Electrophoresis separated the replicated pieces of DNA by size. Staining the gel revealed which lengths of the complementary DNA were terminated by which nucleotide terminators (d). The gel consists of four “lanes,” labeled A, T, G, and C, indicating either adenine, thymine, guanine, or cytosine terminated pieces of DNA. By “reading” down the gel, you…arrow_forward
- Using the same experimental setup as in the original Meselson and Stahl experiment, predict the bands (heavy, light or intermediate) that you would observe after one round of replication if DNA polymerase replicated the bacterial genome by the conservative model of replication. Draw and explain your prediction.arrow_forwardThis is a three part question: Figure B in Box 4.1 illustrates the results of the Meselson-Stahl experiment after a single cycle of replication in 14N. (a) Explain the results they observed after two rounds of replication in 14N medium. (b) Draw out the expected results if a third round of replication were allowed in 14N medium. (c) Two other models for template-directed replication were considered as alternatives to semi-conservative replication. One of these was conservative replication, in which the parental strands were unpaired, replicated, then reannealed such that the parental strands stayed together and the newly synthesized strands were together. The second model was dispersive replication, in which one strand was used as the template for polymerization, then the polymerase switched to using the other strand as the template, and subsequently switched back and forth between the two strands until bother were fully replicated. Each of these models is ruled out by one of your…arrow_forwardSuppose that 28% of the nucleotides in a DNA moleculeare deoxythymidine 5′- monophosphate, and that duringDNA replication the percentage amounts of availablenucleotide bases are 22% A, 22% C, 28% G, and 28% T.Which base would be depleted first in the replicationprocess?arrow_forward
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