Week 5 Lab

Guide Questions: Explain your answer and cite references in APA format. 1. What does mashing do to the fruit? 2. Why did you add detergents? 3. What do you think the ethanol does? Why can't we use room temperature ethanol? 4. To extract DNA from cells, what must you isolate it from in the case of a plant such as strawberry? 5. Look at your container, what do you see in the top portion of the liquid? 6. Is the DNA you extracted is pure? What are the possible impurities? 7. What can we do with the DNA once we have purified it? Discuss different techniques and technologies associated with this. 8. Imagine that there is an E. coli outbreak in your area, and you would like to test the kangkong from your local grocery store. How could you modify this protocol to extract DNA from the kangkong (to identify the species) and check for presence or absence of E. coli.? Keep in mind that (i) E. coli is free-living and not an endosymbiont, and (ii) plant cells are encased in both a cell membrane and…

Titer assay overview questions: 1. What are you measuring when you determine the titer of your lysate? 2. When calculating a titer, would a plate with 12 plaques (pfu) on it be used? Why or why not? (remember pfu= plaque forming units) 3. Calculate the titer for the results below: 10° plate= covered 10 plate= covered 102 plate= 150 pful 10° plate= 18 pfu 10“ plate= 2 pfu

Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) Egg whites contain about 88.5% water and about 10% proteins, from which ovalbumin is the major component. The eggs were cracked open, and the egg whites were separated carefully from the yolk. Stirring of the egg whites was done to break up the membrane. This was followed by filtration through cheesecloth, where a clear filtrate was obtained. About 40.0 mL of the filtered undiluted egg white was measured, and 0.10 mL of 1 M acetic acid was slowly added for every 1.0 mL of egg white with constant stirring. A turbid mixture with few amounts of white precipitate may be expected to form. These may be removed by filtering the mixture using a cheesecloth.   What is the significance of adding 1 M acetic acid to the filtered egg white?

Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation(Salting Out) Egg whites contain about 88.5% water and about 10% proteins, from which ovalbumin is the major component. The eggs were cracked open, and the egg whites were separated carefully from the yolk. Stirring of the egg whites was done to break up the membrane. This was followed by filtration through cheesecloth, where a clear filtrate was obtained. About 40.0 mL of the filtered undiluted egg white was measured, and 0.10 mL of 1 M acetic acid was slowly added for every 1.0 mL of egg white with constant stirring. A turbid mixture with few amounts of white precipitate may be expected to form. These may be removed by filtering the mixture using a cheesecloth After filtration, 30.0 mL of the filtrate was obtained, and the solution was brought from 0% to 40% saturation by adding the required amount of powdered ammonium sulfate, (NH4)2SO4 After adding ammonium sulfate, the mixture was allowed to stand…

Biomedical Engincering Dept. Biocompatibility Hello sir, I know it takes precious Lab. Sheet-Level time. Please, I have a report on this Semester 1 subject. Only 5 papers within a Experiment Title: Cytotoxicity Test discussion solution. I want to solve the text of the keyboard. Thank you, Experiment no. 2 sir 1 12:34 PMA Objectives: Study the eytotoxicity and how it is measured. 1- Background Cytotoxicity can lead healthy living cells to three potential cellular fates. 1. Necrosis (accidental cell death): Rapid loss of membrane integrity and cell lysis 2. Apoptosis (programmed cell death): slower, more orderly, and genetically controlled 3. Cytostasis (a decrease in cell viability): cells remain alive but fail to actively grow and divide Importance of measuring cytotoxicity two major reasons 1. Either you want specific cells to die and look for an adequate compound/condition (cancer and immunotherapy) 2. Or you want to exclude cytoloxicity in specific cells (chemicals and drugs)…

Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation(Salting Out) The computed amount of powdered ammonium sulfate was added to the egg white sample portion byportion with constant stirring while submerged in an ice bath. The solution is expected to become moreturbid, and a white precipitate is expected to form.   Explain the purpose of using powdered ammonium sulfate in the isolation of ovalbumin from egg whites.

Need help fast Q6) Why would a sample be over 100%? Do you think 110% accurate is the same as 90% accurate? Q1) Why are there 2 different ‘stops’ on the micropipette? Q2) There’s many mistakes you can make micropipetting. Describe 3 of themQ4) What does a P100 pipette mean? P20?

ACTIVITY 3: Analyze Me! Direction: Draw a smiling face (O) if the statement is an importance of genetic engineering and (O if not. 1. Genetic engineering has been used to mass-produce insulin. 2. Genes and other genetic information from a wide range of organisms are transformed into bacteria for storage and modification, creating genetically modified bacteria in the process. 3. Some genetically engineered animals become an important source of new drugs. 4. Many genetically engineered crops are being used by many farmers now. 5. Genetic engineering is used to produce more needed genetically engineered medicines.

Bio Mechanics & Ergonomics - II Qno1) A 45 years old patient comes to you with chief complain of neck pain along with stiffness and the pain radiate to the right shoulder and arm. The patient also complained of headaches at the back of the head just above the neck and travel over the top to the forehead. On Further examination it was noticed that the frequency of the pain increased by cervical movement but the severity of the pain is increasing more by looking up to the ceiling the pain is not relieved by any kind of Rest. The previous history of the patient showed that 3 years back he was involved in major car accident. 3)What major changes in cervical spine can be visible on Xray or RMI?

Q21:  Polymerase Chain Reaction (PCR) (Links to an external site.) is an essential technique for the study of DNA. PCR uses a DNA polymerase enzyme from Thermophilus aquaticus (Links to an external site.) to amplify DNA extracted from any sample. In order for the reaction to work, researchers must transfer microliters of sample to the reaction vessel for PCR. If a researcher has a DNA sample with a concentration of 0.2 micrograms per microliter, how many microliters of DNA must be transfered to conduct a 50 microliter reaction if the final concentration should be 2.3 nanograms per microliter? Report your answer to two decimal places using standard decimal notation (e.g. 0.5, not 5.0e-1)

TOPIC: E. coli T4 (T4 Bacteriophage) Include a maximum of 3 sentences, a brief description of their growth. What are the structures responsible for growth and reproduction? What are their physical and nutritional (or chemical) requirements?

Enhancement Questions:  What do you think are the crucial steps in Polymerase Chain Reaction technique? Enumerate the advantage and disadvantage of Polymerase Chain Reaction technique?

topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) Aside from ovalbumin, what are other possible proteins which can be isolated from egg white? Give their biological significance.

present briefly the principle of size exclusion chromatography.  give a brief description of the use of protein assays.

ACTIVITY NO. 15: LUPUS ANTICOAGULANTS 1. What are the clinical criteria for a patient to undergo tests for lupus anticoagulant? 2. Illustrate the steps of mixing studies specified for lupus anticoagulant. 3. Illustrate the steps of parallel assays for lupus anticoagulant.

Adaptive Learning by PrepU Question 2 of 5 A 3-year-old child is to receive 500 ml of dextrose 5% in normal saline (D5NSS) solution over 8 hours. At what rate (in milliliters/hour) would the nurse set the infusion pump? Record your answer using one decimal place.

Topic: Isolation of E. coli bacteriophage   What would happen if: - the enrichment method in the isolation of bacteriophage was omitted? - the chloroform was not added to the enrichment? - the 0.1 ml lysate-E. coli mix was plated directly on top of the bottom agar?

Topic:  Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) The computed amount of powdered ammonium sulfate was added to the egg white sample portion byportion with constant stirring while submerged in an ice bath. The solution is expected to become moreturbid, and a white precipitate is expected to form. The resulting mixture was then filtered using a cheesecloth. The residue was discarded, and 30.0 mL of thefiltrate was brought from 40% to 60% saturation by adding the required amount of powdered ammoniumsulfate in the same manner as the previous addition.  After adding ammonium sulfate, the mixture was allowed to stand with occasional stirring for 30 minutes inan ice bath. The formation of a white precipitate is expected to happen QUESTION: Explain the significance of allowing the mixture to be submerged in an ice bath for a given time.

Why clinical trials are necessary? Explain various clinical trial designs and phases of clinical trials.

The correct order of steps when purifying a protein are: Group of answer choices 1)Growing bacterial culture for fusion protein; 2)Harvesting IPTG-induced cultures; 3)Lysing bacterial cell; 4)Removing insoluble debris; 5)Using Affinity Chromatography to purify protein.   1)Growing bacterial culture for fusion protein; 2) Breaking open bacterial cells; 3) Removing insoluble debris: 4) Harvesting IPTG-induced cultures; 5)Using Affinity Chromatography to purify protein   1) Growing bacterial culture for fusion protein; 2) Lysing bacterial cells; 3) Harvesting IPTG-induced cultures; 4) Removing insoluble debris: 5)Purifying protein by affinity chromatography   1)Growing bacterial culture for fusion protein; 2) Removing insoluble debris; 3) Harvesting IPTG-induced cultures; 4)Breaking open bacterial cells; 5)Purifying protein by affinity chromatography

Discuss shortly five important things that should be kept in mind when designing a centrifugation protocol?

Question:- How to perform a bactericidal test bactericidal test of Dental Pulp Stem Cell (DPSC)?

Please answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated.   Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurred

Microbial sensivity lab: Why are Escherichia coli, Pseudomonas aeruginosa, and StaphyLococcus aureus used as standards in the Kirby­ Bauer method?

Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. Show some sample computation.  Concentration  (micrograms/ml) A595 - trial 1 A595 - trial 2 A595 - trial 3 average A595 A595 of sample minus A595 of blank 0 1.501 1.446 1.447     2 1.624 1.558 1.559     5 1.731 1.749 1.712     10 1.901 1.838 1.892     15 2.161 2.108 2.228     18 2.231 2.277 2.319

Think about minimal inhibitory concentration (MIC) assays.  A set of tubes, all containing the same amount of bacterial cells, have decreasing amount of antibiotic X added to them.  Tube 1 had 0mg/ml, Tube 2 has 100mg/ml, tube 3 has 50mg/ml, 4=25, 5=12.5, 6=6.25, and tube 7 has 3.125.  If bacteria grew in tubes 1, 4, 5, 6, 7, what is the MIC of this antibiotic for this bacteria? Select one: a.None of the Above b.3.125 mg/ml c.25 mg/ml d.0 mg/ml e.50 mg/ml

Explain the basic principles of Elispot Assay

5G 14:32 l 61% 5 Component Lab report... Return Submit work. 1 3 points What is the role of lysozyme and a low ionic strength extraction buffer? Select all that apply. Lysozyme is an enzyme found in lysosomes in cells Cells absorb water from a low ionic strength extraction buffer, by osmosis, swell and lyse, releasing their contents Lysozyme breaks down bonds in the peptidoglycan cell wall of bacteria, weakening the structure Lysozyme is an antibiotic found in tears, saliva, human milk and mucus. Cells release water into a low ionic strength extraction buffer by osmosis, shrink and lyse, releasing their contents Lysozyme breaks down bonds in the cellulose cell wall of plants, weakening the structure 2 1 point Why is everything stored on ice? We are working to extrat keeping the samples on and down ofcells helning in the procecs

Briefly outline &/or sketch the premise of the transwell assay:

hello sir    xplain why it is important to evaluate or measure blood compatibility with biomaterial???

Activity Consider the following study: A new health-threatening virus, SHV, has been identified and a research group at the Northern Virginia University (NVU) is developing the vaccine. The scientists at NVU are testing the following hypothesis: people who receive the SHV vaccine do not get the virus. In the clinical trials, one group of test subjects is treated with the vaccine, the other group is given the placebo, a shot containing saline solution without a drug. Both groups have the same number of individuals with identical sex ratio, age range, body type, and similar medical history. Upon administering the vaccine, the researchers measure how many individuals get infected with SHV on a monthly basis in a period of one year. The same experiment is conducted concurrently in several other leading institutions across the country.         1-What is the experimental group in this experiment?   The experimental group is Select an answer and submit. For keyboard…

ELISA METHOD What are the names of the four major types of ELISA?