TheHotZoneQuestions Part 2

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Apr 29, 2024

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Epidemics and Plagues Reading: The Hot Zone _________________________________________________________________ As you read the assigned chapters for your book this week, answer the following questions. The questions are arranged by section, and you will be able to find the answers to the questions in that particular section. Please write your answers in a different color. Part 2: The Monkey House 1) What kinds of monkeys were kept at the Reston Monkey House? A type of wild-crab-eating monkey from the Philippines named long-tailed macaques. 2) How many monkeys out of the original shipment were dead within one month? 29 3) Describe the deaths of the monkeys. They typically became lethargic and anorexic. Postmortem examination revealed enlarged and hardened spleens resembling blood clots. 3) Who is Peter Jahrling? Virologist at USARIID who specialized in monkey viruses. 4) How did Dan Delgard ship the results of the monkey autopsy to Jahrling? He wrapped it in aluminum foil 5) What did Tom Geisbert observe when he first looked at the flasks of monkey cells? The cells appeared unhealthy, with milky and clouded fluid containing dead and exploded cells which was unlike the standard SHF cells. 7) What did Peter Jahrling say about these cells? He said that they were contaminated. 8) What happened over the Thanksgiving holiday to the monkeys in the monkey house, and why was it significant? The 5 monkeys died in room H which proved that the virus was spreading. 9) Why did Geisbert use a diamond knife in the lab? To cut thin sections of coagulated monkey cells 10) Why did Geisbert use an eyelash in the lab? It was used to separate sections floating in water. 11) Using the electron microscope, what discovery did Geisbert make about the viruses infecting the monkey cells? He saw a distinct structure resembling a filovirus. 12) What are inclusion bodies? Clusters of virus particles within cells. 13) What is the “Slammer”? Refers to the level 4 biocontainment hospital 14) What other test did Jahrling and Geisbert perform, and why? They tested their blood to determine if they were infected with what they believed to be Marburg from other contaminated cells. 15) What were Geisbert’s microscopy results from the infected monkey liver? It showed signs of viral proliferation, with snake-like structures. 16) Describe the test Jahrling performed on the infected cells to confirm the identity of the virus.

Epidemics and Plagues He used UV to examine blood from infected humans that had Marburg, Sudan, and Zaire Ebola. 17) What were Jarhling’s results from the test he performed on the infected cells? They glowed which indicated a positive est. 18) What is meant by a “chain of command” in relation to biohazard work? Reporting to higher-level personnel to ensure proper protocol and procedures. 19) How were the dead monkeys taken to Fort Detrick? They were double-bagged in garbage bags. 20) What were the results of the monkey autopsy? Inconclusive 21) Who is Joe McCormick? He is the Chief of the Special Pathogens Branch of the CDC. 22) What were the three priorities defined for the biohazard operation involving the monkey house? Safety of the human population, euthanasia with minimal suffering, and collecting samples to identify strain and transmission. 23) Describe the conditions of the monkey house upon the team entering the building. Strong odor and workers refused to clean the cages out of fear of contracting the virus. It was basically mayhem.

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