Lab Ex 3-7-Acid-Fast Stain

Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) 1. Why do you destain the gel? 2. What is a gradient gel? Why are we using them? 3. Compare and contrast SDS-PAGE and native PAGE. Why would we want to do each of these techniques?

Non-enzymatic browning Proteins: Lysin, methionine Sugars: glucose, galactose, sucrose, fructose, maltose 1. Describe (the aromas produced) and the degree of browning for each solution 2. Explain variations in color with respect to each sugar-amino acid combination by taking the chemical structures of sugars and amino acids into consideration

When run under nondenaturing conditions (without SDS or β-mercaptoethanol), nativepolyacrylamide gel electrophoresis (PAGE) allows proteins to maintain biological activity, so the GFP band should fluoresce when exposed to UV light. How do the staining patterns of gels run in this way differ from the staining patterns of gels run using SDS-PAGE?

Hello, good day. I am having a problem answering this question and I need your help. Hoping for a response and thank you so much. Question: What are the general rules in grams staining classification of bacteria?

True or false?  each objective of a phase contrast microscope contains a phase plate.   most cultured mammalian cells grow best at 37C.     the oculars on the compound light microscope are typically 10X.

1. Isoelectric focusing. A digested protein sample was applied to one end of a gel strip with an immobilized pH gradient. According to the experimental image from the results by gel running. Please indicated that what amino acids were compositionally enriched in separated dot 1 and 2? pH 9 Dot An electric field is applied Dot Decreasing pl pH 3 After staining, proteins are shown to be distributed along pH gradient according to their pl values.

Agarose gels can vary from 0.8 to 2% explain why we use different gel percentages

Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.

Part a. You making 30 mo of agarose gel. Sybersafe A concentration 1000x. How much you will add......ul to the gel. b. You will be loading 20 your diagnostic digest onto your gal. The loading dye you have been provided is 5x concentrated. You will add..... ? ul to your sample before loading your gel.

Gradient PAGE uses differing gel concentrations along the length of the gel to achieve optimalseparation of proteins of a wide range of molecular weight proteins. How does the staining pattern of these types of gels differ from nongradient PAGE?

Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) 1. What determines the current in your gel? What could cause the current in your gel unit to be lower than expected? Is there anything that could cause it to be higher than expected? 2.  Why do you need to wash the gel before staining it? Why use warm water?

56/take Select all that apply to a direct simple stain: O uses only 1 dye in the procedure O uses multiple dyes in the procedure O can be used to determine cell morphology, size, and arrangement. does not involve fixing does involve fixing does not involve a washing step does involve a washing step O the dye is basic or positively charged and stains the bacteria O the dye is acidic or negatively charged and stains the background O cells may be distorted or shrunken Ocells will never be distorted or shrunken

For various species or portions of organisms, different stains have different affinities. Explain this assertion using the example of hematoxylin and eosin staining.

Why is agarose an ideal gel matrix for electrophoresis experiments? Because it forms a liquid at all temperatures. Because it is un-ionized and uncharged Because it is positively charged fs f6 f7 fg fg f1o $ [8 19 4 6 7 8. 4. 5 R Y F G J 2 K エ

Phase contrast microscopy - Human Cheek Cells a. b. What is the basic principle of image formation using this microscopy technique? What can be observed and concluded from the image of the specimen?

For a TLC experiment, what is the purpose of the pentane extraction of spinach juice? Why wouldn’t we want to spot an aqueous solution onto the silica gel?

Why do we use a stacking gel in SDS-PAGE

Oops!  Your pipettor wasn’t set correctly and you only pipetted 87 µl of a 10-7 dilution when plating serial dilutions.  But, you noted this error in your lab notebook so you could correctly perform the calculation.  When this plate was grown, you counted 147 colonies on the plate.  What was the concentration of bacteria in the original, undiluted suspension?

Please help using the image i attached below. There are 3 parts to this question. Describe how this is related to light scattering. Note the field is always dark in this type of microscope (and hence, called dark-field). Why is this? Explain how this type of microscope can be used to detect (but not resolve) flagella.

Which of the following technique is FALSE in microscopy? Osmium tetroxide can be used as a fixative as well as a negative stain in transmission electron microscopy. Two proteins; each tagged with their individual primary antibody followed by one protein with rhodamine conjugated secondary antibody and the other with fluorescein-conjugated secondary antibody can be visualized simultaneously using a fluorescence microscope. The emission spectrum of Rhodamine is 580 nm The emission spectrum of Fluorescein is 521 nm O An objective lens with 100X magnification and projection lens with 10X magnification is going to produce a 1000X total magnification. Phase contrast and differential interference contrast (DIC) do not require staining.

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PLS ANSWER ALL 1. Why were nuclei being unstained when you used eosin? 2. Aside from H&E, what other staining methods are applicable in Golgi apparatus, mitochondria, and nucleic acid? What are the differences?

21 22 23 24 Short Essay: Answer the prompt in 4 sentances or less. Describe the staining technique used to create the photo. Make sure to describe the structures indicate by the arrow in letter A and the structures circled in letter B. Is this a simple or differential stain? Explain your answer. 1- OB 10 μm

Acetaminophen Elixir 120 mg/5 mL   During the Covid-19 epidemic, the public were advised high fever was a common side effect of infection and to ensure they had acetaminophen on hand. Because of this, there was a sudden nation-wide shortage of commercial liquid acetaminophen. The local Doctor asked if you could compound a mixture for a young child with otitis media as the Mother cannot find any commercially. The Pharmacist did some research and found the following referenced formula for you to use. The Doctor would like to give 50 mL. The child weighs 16 kg.   Formulation:   Acetaminophen 120 mg 90% ethanol 0.7 mL Propylene glycol 0.5 mL Purified water 0.5 mL Glycerol qs ad 5 mL   Dissolve acetaminophen in ethanol and propylene glycol, add most of the glycerol, add water, adjust to volume with glycerol.   Strength: 120 mg/5 mL Child dose: 15 mg/kg q4-6h. Maximum 4 doses in 24 hours. Warnings: Contains 13% alcohol EUD: As per local guidelines.   Questions:   Calculate appropriate…

When combining 1ml of lysosome solution with a concentration of 5mg/ml and 1ml of water what is the final concentration of the lysosome solution after adding 8.0 of a biuret reagent to the solution  2.5mg/ml 1mg/ml 0.5mg/ml 5 mg/ml

Multiple choice (Refer to picture)   Which of the statements is NOT TRUE about the sample in Salkowski test? * Lower layer becomes red with a green fluorescence A molecule of cholesterol reacts with sulfuric acid to yield bi-cholestadien There should be an excess sulfuric acid to convert bi-cholestadien to bisulfonic acid of bi-cholestadien. It is best to shake the solution for a more accurate result.

Histopathology- Tissue processing STAINING OF SECTION (H and E STAINING) State the purpose of the following in staining: A. XYLENE 1 AND 2 B. Alcohol Absolute to 50 % Alcohol C. Hematoxylin D. Acid Alcohol - E. Ammonia Water F. Alcohol from 50 % to Absolute alcohol G. Last 3 Xylene

write true if the statement if correct and change the " " word/phrase to make it correct irradiating UV light to a chromatogram that is sprayed with a "2'7'-dichlorofluorescein" solution will show phospholipids as red-purple spots on a pink background

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SDS-PAGE gels are useful in determining the molecular weights of proteins; however, the molecular weights of are usually not reliable. protei

Liquid supported membrane of DGA used for rare earth elements separation. Tell me the background/introduction of this research, current state of the art, motivation, how these membrane are made , what is the appropriate characteristics of them, approaches that author followed, you can describe the problems of that approaches, why rare earth is challenging to separate, why current state of the art do not address that need, include two or 3 results and describe it. take those information from few journal papers and mention the correct citation.