Bacterial Growth Report Only
.docx
keyboard_arrow_up
School
Montgomery College *
*We aren’t endorsed by this school
Course
210
Subject
Biology
Date
May 4, 2024
Type
docx
Pages
4
Uploaded by MasterHedgehogMaster950 on coursehero.com
Bacterial Growth Report (21.0 pts.): Bacterial Growth Curve Using Serial Dilution With Viable Plate Count & Absorbance Name: Katherine Benitez USE Given Lab DATA IN EXCEL TABLE TO PLOT ABSORBANCE AND LOG CFU/ML (WITH 2 Y-AXES) OVER TIME. Note that there are two dependent variables. (Connect data points and do not forget TAILS!)
(i) Plot absorbance @ 600nm (left Y-axis) vs. time …. (3.5 pts.)
(ii) On same graph plot log cfu/ml (right Y- axis) vs. time ….. (3.5 pts.)
(iii) Answer questions below
(iv) Attach your graph and submit
***How to draw a graph using Excel: https://youtu.be/DM52xjSLGqs
https://www.youtube.com/watch?v=DM52xjSLGqs&feature=youtu.be
***Excel - how to plot a line graph with 2 vertical y-axis: https://www.youtube.com/watch?v=P-mB4I16GC8
1.
Write the formula for calculating cfu/ml. Use a countable plate from the image below and show calculations to obtain cfu/ml from the original stock culture (3.0 pts). From left to right, serial dilution tubes of: 1
0
-3
, 10
-4
, 10
-5
, 10
-6
, 10
-7
were used to distribute bacteria on the plates below. 0.15 mL of each tube dilution was used to spread out on each plate.
Formula for calculating CFUs/mL (1 pt.)
Number of Colony Forming Units x Dilution Factor = CFU/mL
*Calculations for CFUs/mL (3 pts.): 159x10^5x0.15=
1.59x10^7= 15,900,000
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
- Access to all documents
- Unlimited textbook solutions
- 24/7 expert homework help
Related Questions
and the minimum exposure time
1. Determine the minimum inhibitory concentration
given the following data:
Concentration of
ethanol
Number of colonies of E. coli on Nutrient Agar plates
Exposure time (in minutes)
10
5
15
Distilled water
+++
+++
+++
40%
+++
++
+
70%
95%
Minimum inhibitory concentration:
Minimum exposure time:
arrow_forward
DILUTION
COLONY COUNT
CFU/mL
1:106
155000
1:107
15500
1:108
1550
1:109
155
Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?
arrow_forward
Apple puree was analyzed for petulin by HPLC-MS-MS after SPE clean-up. The procedure was 10.0g of puree + 10μl of a 10μg/ml solution of isotopically labeled petulin as internal standard were treated with 10.0ml of pectinase and acetic acid, centrifuged, and filtered. Four ml of the filtrate was passed through a SPE cartridge. The petulin was eluted with 2.0ml of ethyl acetate. The sample was evaporated to dryness and the residue dissolved in 1.0ml of the mobile phase. The analyte signal was 127 and the internal standard 197. Calculate the concentration of petulin in the sample in μg/g (RRF=1).
arrow_forward
Confluence cells added onto 100-wells
plate, 2 ml trypsin added onto cells and 6 ml
media added to dilute trypsin, 1 ml was used
for counting, and average number of Cells
counted was 24(DF 2)
find out the the number of cells/ml and total
number of cells in 7 ml?
How many cells you need for 100 wells,
assuming Number of cells/well is 10 to the
power 4 cells?
arrow_forward
What is your overall dilution factor if you complete 3 serial dilutions using a 100-fold dilution each time? (Show your work)
arrow_forward
Determine the minimum inhibitory concentration
and the
minimum exposure time
given the following data:
Number of colonies of E. coli on Nutrient Agar plates
Exposure time (in minutes)
Concentration of ethanol
10
15
Distilled water
+++
+++
+++
40%
+++
++
70%
95%
Minimum inhibitory concentration (MIC):
Minimum exposure time (MET):
arrow_forward
topic: Isoelectric Precipitation
Appearance of the mixtures containing casein mixed with different buffer solutions: (a) TestTube 1 containing Glycine-HCl buffer; (b) Test Tube 2 containing Acetate buffer; and (c) Test Tube 3containing Phosphate buffer.
QUESTION:
Based on the given results, what do you think is the isoelectric pH of casein? Briefly discuss your basis for determining it.
arrow_forward
1&3/4 tbs po BID x 7 days #QS
How many milliliters should the pharmacy dispense?
arrow_forward
Standard curves
You are setting up a standard curve for lysozyme. You have a stock vial containing 2 mg/ml
of lysozyme and you have 2 ml in the vial. You will want to have a minimum of 1 ml of each
concentration for the spectrophotometer. How would you proceed? (Saline is added to make
the various concentrations).
Concentration Am't stock lysozyme or am't of a
previous dilution (note which)
Amount saline
Total volume
wanted
1.00 mg/ml
0.75 mg/ml
0.50 mg/ml
0.375 mg/ml
arrow_forward
Calculate the IV rate in drops per minute for a 1 L bag of Lactated Ringers solution running at 50ml/he using the 15 drop factor?
arrow_forward
ent id%= 20737
ning Time: 28 minutes, 53 seconds.
ion Completion Status:
turned
red after
Zn
Stayed
colorless
after Zn
turned red after
NI and NII
Which bacteria possess potent (strong) nitrate reductase ?
Pseudomonas aeruginosa
Staphylococcus epidermidis
Streptococcus pneumoniae
E.coli
dtv
MacBook Air
O O O C
Streptococcus
pneumoniae
Staphylococcus
epidermidis
Escherichia coli
Psеudomonas
aeruginosa
arrow_forward
procedure/s in performing aseptic transfer of bacterial cultures in (include illustration)
(1) broth culture to broth
arrow_forward
Interpretation:
> Determine the total number of bacteria per plaque sample and toothbrush head from BHI.
Number of colonies on a selected plate
CFU/ mL =
Total dilution x amount plated
Questions:
Results of the plates using the plaque sample
Q1: Which of the 6 BHI plates
-1
10
10 2
shown would be the one to
use as your count for the
formula?
TMTC
TMTC
TMTC
Q2: Set up the formula and solve
for # of CFUS per plaque
sample.
10
238 colonies
24 colonles
2 colonies
arrow_forward
Patch clamp
What is patch clamp technique?
Identify two features of the patch clamp technique that make it a useful tool in studying cellular electrophysiology. Explain each thoroughly.
What is the role of the reference electrode in the patch clamp technique? Discuss how it works.
arrow_forward
in food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you
if given:
Temperature = 75 C
Molar absorptivity coefficient = 10.5
Path length (cm) = 2
I = transmitted light = 0.45
arrow_forward
Briefly explain that what is the important to use immobilization techniques in biosensor?..(explain
under5 points)
arrow_forward
1. (2.5 pts) A pure bacterial culture of unknown concentration was diluted to determine the
concentration of viable bacteria in the original culture. Serial dilutions were performed as
diagrammed below. Each dilution tube contained 400 ul of diluent and 100 ul was transferred
into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes.
a. What is the dilution between each tube shown in the diagram below? Express your
answer as a ratio.
b. What is the total dilution of tube number 5? Express your answer as a ratio.
c. What is the concentration of viable bacteria in the original culture? Express your
answer using scientific notation and the units CFU/ml.
d. What is the concentration of viable bacteria in tube number 2? Express your answer
using the units CFU/ml.
e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would
you expect to see after the plate was incubated?
1
2
3
5
423
80
13
Number of colonies
arrow_forward
Sulfur Indole Motility (SIM) Medium
H2S produced, color and +/-: ______________________________
Indole present/Tryptophan hydrolysis, color and +/-: ___________________________
Motile or non-motile: _____________________________
arrow_forward
Table 1 - Comparison of the effect of catechol concentration on the amount of product formed.
Absorbance
Potato
extract
Absorbance
0 mins
after 30mins (2nd reading)
(mL)
1st reading
1
Tube #
la
blank
2a
3a
4a
1
1
1
dH₂O Catechol
(mL) (mL)
7
5
3
1
0
2
4
6
0.00
0.060
0.033
0-05-2
Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1?
Time for reading: 3:21
-0.11
Absorbance:
Time for reading: 3.36
Q5) Tube la serves as a control, but why is this control needed?
Absorbance:
0.197
Time for reading: 3.37
Based on the data from Table 1 answer these questions:
Q1) What is the name of the enzyme found in potato extract? Answer:
catechol
Q2) What is the substrate? Answer:
THO
Q3) Name of product of this enzyme catalyzed reaction? Answer:
Absorbance: 0.152
Time for reading: 3:39
Absorbance: . 166
ness
Catechol
Benzoquinone
Subtract 1st
from 2nd
reading
-0.01
0-137
0.11.19
0.119
Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different…
arrow_forward
Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?
arrow_forward
List the ingredients and recipe (in gram or milligram) to make one liter (1000 ml) of Trypticase Soy Agar, nutrient agar, and Columbia blood medium (use a table
arrow_forward
based on the chart answer the following:
1. which proteins will be most excluding use gel filtration chromotagrpahy
2. which proteins will migrate closer to the anode using SDS- PAGE
3. which protein will have the most net positive charge at a ph of 7.4
arrow_forward
HW....... Q1/: Compare the contact time necessary to obtain 99.99% kill of bacteria in water under the following conditions: 1- Free iodine residual of 0.45 mg/L and k=1.1* 10²/s. 2- Combined iodine residual of 8 mg/L with k= 1.2* 10²/s.
arrow_forward
Experiment Title : Surface Modification of poly(vinylidene fluoride) (PVDF) membranes with untreated and treated chitosan for antibacterial performance.
QUESTION: From the given title, please make a clear discussion about antibacterial perfomance
arrow_forward
Question: Determine the amount of dehydrated medium needed to prepare 15 butts, 20 slants, and 25 butt-slants. Include amount for 2 additional units of each as excess to compensate for compounding losses.
arrow_forward
AE, 62, Male
Wt: 75 kg, Ht: 150 cm
Orders:
Penicillin G Potassium 10,000,000 Units
Sterile water for injection qs
Make a 250,000 U/mL solution.
The package insert states If Penicillin G Potassium 10,000,000 Units is reconstituted with 44 mL sterile water, a solution of 200,000 U per mL will be obtained.
What volume of sterile water would you use to reconstitute the penicillin G potassium in order to make the ordered solution?
arrow_forward
a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this sample were plated out, these would theoretically be 10,000 colonies in the Petri plate. Discuss and explain the serial dilutions of this example.
b.
The disk diffusion method was used to evaluate 3 disinfectants. The results were as follows:
Solution
Zone of inhibition
X
0 mm
Y
5 mm
Z
10 mm
How would you interpret? Provide inference.
arrow_forward
Serial dilution = previous dilution x dilution
11 mL
[2]
3 mL
Colony count:
100
0.1 mL
sample
[1]
9.9 mL
5 mL
0.1 ml.
Cokony count
Dilution:
10-1
10-3
[3]
150
Dilution factor (DF): [4]
[5]
[6]
Compute for the CFU/mL of the sample. Show your solution and express your final
answer to 2 significant figures.
arrow_forward
Consider the sterilization of a sodium gluconate production medium in the holding section of a continuous sterilizer. Assuming constant temperature, the specific death rate constant of the contaminant is 20 s-1 . If the average residence time in the holding section is 10 seconds, calculate the Del factor for the following and explain the results. i. For Pe = 400 ii. For Pe = 400, assuming plug flow iii. For Pe = 100 iv. For Pe = 100, assuming plug flow
arrow_forward
Observe the following Plate counts and then determine the correct number of CFU/ml
Plate 1 = 564 colonies at 10^-5 dilution
Plate 2 = 422 colonies at 10^-6 dilution
Plate 3 = 317 colonies at 10^-7 dilution
Plate 4 = 93 colonies at 10^-8 dilution
93 x 10^10 CFU/ml
9.3 x 10^-9 CFU/ml
93 x 10^9 CFU/ml
93x 10^8 CFÜ/ml
93x 10^-8 CFU/ml
asap please
arrow_forward
SEE MORE QUESTIONS
Recommended textbooks for you
Related Questions
- and the minimum exposure time 1. Determine the minimum inhibitory concentration given the following data: Concentration of ethanol Number of colonies of E. coli on Nutrient Agar plates Exposure time (in minutes) 10 5 15 Distilled water +++ +++ +++ 40% +++ ++ + 70% 95% Minimum inhibitory concentration: Minimum exposure time:arrow_forwardDILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?arrow_forwardApple puree was analyzed for petulin by HPLC-MS-MS after SPE clean-up. The procedure was 10.0g of puree + 10μl of a 10μg/ml solution of isotopically labeled petulin as internal standard were treated with 10.0ml of pectinase and acetic acid, centrifuged, and filtered. Four ml of the filtrate was passed through a SPE cartridge. The petulin was eluted with 2.0ml of ethyl acetate. The sample was evaporated to dryness and the residue dissolved in 1.0ml of the mobile phase. The analyte signal was 127 and the internal standard 197. Calculate the concentration of petulin in the sample in μg/g (RRF=1).arrow_forward
- Confluence cells added onto 100-wells plate, 2 ml trypsin added onto cells and 6 ml media added to dilute trypsin, 1 ml was used for counting, and average number of Cells counted was 24(DF 2) find out the the number of cells/ml and total number of cells in 7 ml? How many cells you need for 100 wells, assuming Number of cells/well is 10 to the power 4 cells?arrow_forwardWhat is your overall dilution factor if you complete 3 serial dilutions using a 100-fold dilution each time? (Show your work)arrow_forwardDetermine the minimum inhibitory concentration and the minimum exposure time given the following data: Number of colonies of E. coli on Nutrient Agar plates Exposure time (in minutes) Concentration of ethanol 10 15 Distilled water +++ +++ +++ 40% +++ ++ 70% 95% Minimum inhibitory concentration (MIC): Minimum exposure time (MET):arrow_forward
- topic: Isoelectric Precipitation Appearance of the mixtures containing casein mixed with different buffer solutions: (a) TestTube 1 containing Glycine-HCl buffer; (b) Test Tube 2 containing Acetate buffer; and (c) Test Tube 3containing Phosphate buffer. QUESTION: Based on the given results, what do you think is the isoelectric pH of casein? Briefly discuss your basis for determining it.arrow_forward1&3/4 tbs po BID x 7 days #QS How many milliliters should the pharmacy dispense?arrow_forwardStandard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2 mg/ml of lysozyme and you have 2 ml in the vial. You will want to have a minimum of 1 ml of each concentration for the spectrophotometer. How would you proceed? (Saline is added to make the various concentrations). Concentration Am't stock lysozyme or am't of a previous dilution (note which) Amount saline Total volume wanted 1.00 mg/ml 0.75 mg/ml 0.50 mg/ml 0.375 mg/mlarrow_forward
- Calculate the IV rate in drops per minute for a 1 L bag of Lactated Ringers solution running at 50ml/he using the 15 drop factor?arrow_forwardent id%= 20737 ning Time: 28 minutes, 53 seconds. ion Completion Status: turned red after Zn Stayed colorless after Zn turned red after NI and NII Which bacteria possess potent (strong) nitrate reductase ? Pseudomonas aeruginosa Staphylococcus epidermidis Streptococcus pneumoniae E.coli dtv MacBook Air O O O C Streptococcus pneumoniae Staphylococcus epidermidis Escherichia coli Psеudomonas aeruginosaarrow_forwardprocedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (1) broth culture to brotharrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you