An Experiment On Synthesized Luminol

To begin the lab, two dry and clean 125ml Erlenmeyer flasks are weighed separately on a top loading balance with a piece of 2.5” x 2.5” aluminum foil and an elastic band. After weighing, 2ml of the unknown organic liquid sample is poured into each of the Erlenmeyer flasks and secured with the aluminum foil that is wrapped around the mouth of the flask and secured with the elastic band. After the smaller set of aluminum foil has been secured, the larger pieces of aluminum foil, 3.5” x 3.5”, is then secured with an elastic band over the previously secured aluminum foil. Two water baths are placed on hot plates in 600ml beakers filled up to 200ml with distilled water, the stir bars are placed in the 600ml beakers and rotational speed is increased.

years ago. Luminol is so sensitive that it can detect blood at one part per million. Luminol is known to florence differently when it reacts to blood, copper, certain bleaches, and plant matter. The way temperature in a room can affect the brightness of the chemical luminol. This research paper will provide information on the chemical luminol and temperature.

Approximately 160 uL of p-cresol, 260 uL of 25% NaOH solution, and 0.019g of tetrabutylammonium were used. While the p-cresol, 25 % aqueous NaOH and tetrabutylammonium bromide solution was being heated it slowly turned yellow and then eventually turned a light brown shade. It was originally a colorless liquid. When this solution was being heated, the temperature remained steady around 95-100°C. It was heated for 60 minutes. When ether was added to the conical reaction vial after its contents were heated, three layers formed. The top layer was colorless, the middle layer was white, and the bottom layer was light brown. The top layer was the organic (ether) layer that was then removed via pipette. Approximately 400 uL of 5% aqueous sodium hydroxide

After the hot solvent was cooled down, a gravity filtration was performed to remove impurities

Experiment 2: The second experiment methods and materials are listed in the lab manual Wolven, 2014; Montezuma

At the beginning, a two-hundred and fifty milliliter (mL) beaker was obtained and two-hundred milliliters of distilled water (H2O) was added. With a plastic pipette, eight drops of Iron(III) nitrate (Fe(NO)3), and eight drops of Ammonium thiocyanate (NH4NCS) were added to the distilled water. The substance was then mixed with a

This article Colorful Chemistry, is a lesson written by P. Teal Sullivan, Carsten Conner, Mareca Guthrie, Stephen Pompea, Blakely Tsurusaki, and Carrie Tzou, that incorporates art and science together through chemistry (p. 35, 2017). Art and science go together quite well in regards to chemistry, especially when we are talking about the color in our world. In order to create colors, cavemen had to make chemical reactions that could create those different pigments or colors (Sullivan et al., p. 35, 2017). Throughout this lesson, students will used acids and bases to change the color of red cabbage pigments, in order to show, that mixing substances can produce a chemical reaction.

HCl was added to the filtrate until acidic to litmus paper, then the solid was collected by vacuum filtration and washed with water.

Glow sticks contain two separate sections inside them. Both sections contain two different chemical solutions. When broken, the hydrogen peroxide solution mixes with diphenyl oxalate. The diphenyl oxalate is then oxidised by the hydrogen peroxide which then produces dioxetanedione. When this happen, the chemical dioxetanedione releases enough energy to excite the electrons in the fluorescent dye to a excited state. When this process happens, it is called chemiluminescence which also releases or emits photons. In chemiluminescence, when the electrons go to a higher energy orbital then coming back down to its original, the glow stick is releasing electromagnetic radiation. There is a certain wavelength that is being released which is visible

Purpose: Using a spectrophotometer to measure and evaluate the effect that changing the concentration has on the intensity of fluorescence in turmeric powder.

From Criminal Minds to CSI, everyone has watched the criminal investigation shows on television and loved watching gorgeous actors play scientists, who in the real scientific world would have no clue as to what they’re doing but surely we have all been curious, “How do they make blood light up?” The whole idea of forensic science has always interested people and that is what keeps those television shows running. The key to getting and creating any chemical reaction that glows is just a few chemicals at the right temperature and a few simple substances that can transform a simple concoction into an eerie looking splotch of blood or human fluid.

At the end of reflux, both the reactants and products are present in the pear-shaped flask. This is because the acid used was very weak and did not fully react with the alcohol therefore once the mixture has reached a chemical equilibrium, the concentration will remain the same. This means that not all the reactants are going to be converted into products. Washing the mixture with 20mL of distilled water allowed the separation between the aqueous layer (water-soluble substances) and the organic layer (ester) in the separating funnel. Therefore washing the mixture with distilled water is needed to separate the aqueous layer, which is discarded, with the ester.

After reflux, the solution was washed with water because isopentyl acetate is insoluble in water whereas acetic acid and sulfuric acid are both water soluble. Washing the product with water got rid of most the unreacted starting materials, but another wash with sodium bicarbonate was needed to completely get rid of them. A wash with sodium bicarbonate converted the

The apparatus was set-up in fume hood. After adding 5ml of acetic anhydride and five drops of 85% phosphoric acid into 50ml Erlenmeyer flask which contained 2.001g salicylic acid, the flask was heated on a hot plate (75℃ ) for 15 minutes while stirring the flask’s content. A butcher funnel was then set-up for filtration of the flask’s content.

Without letting the column run dry, hexane:acetone (1:1) was introduced into the column, and in the same manner the eluates were collected. This was the same for the succeeding eluents, and went on until no more colored eluates could be obtained from the column.