Bacterial Unknown Lab Report

An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The first procedure that was done was the gram stain. Under a microscope, if the gram stain is purple, the bacterium is gram positive, if the stain is red, it is gram negative. The next test was the fermentation tests for glucose, sucrose and

The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes.

The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others make antibiotics used today. This lab was completed by using the methods learned thus far in identification of bacteria.

The purpose of this study project was to carefully isolate and identify two unknown bacteria from a mixed culture. The ability to properly evaluate biochemical test results is also necessary for the identification to be successful. The goal was to apply all of the methods and techniques that have been learned in the microbiology laboratory course for the proper identification of unknown bacteria. A certain amount of bacteria that were used throughout the course were possible bacteria that could be found in a mixed culture. The bacteria that were identified in the mixed culture were Staphylococcus Aureus and Kocuria Rhizophila.

In a laboratory setting, it often becomes necessary to identify an unknown organism. In this experiment, researchers classified an unidentified bacterium based on its physical structure, colony morphology, optimal conditions and metabolic properties. A Gram stain using crystal violet, iodine, and safranin and a simple stain using methylene blue characterized the organism’s cell wall. Cultural behavior was classified by inoculating the organism onto nutrient agar and incubating it at 37° C for 48 hours, and observing its behavior, as well as using SIM medium to test for motility. Optimal growth temperature was

The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible

There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of unknown bacteria. The identification process can be completed with a series of deferential stains and biochemical tests. Creating a dichotomous key helps to limit the amount of biochemical tests done on an unknown organism and by observation

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed.

During the final experiment for Microbiology will have to figure out the unknown organism given to us. The organisms will be narrowed down by using skills we have learned throughout the lab this quarter and the test results will narrow down to a single organism. The reason to perform experiments that determines the identity of bacterium is to find out how to treat it or what infections it is causing. In a controlled environment, students learned how to use various methods to determine how to differentiate between multiple bacteria. This lab report will be detailed steps explaining how to determine the identity of my unknown organism number 12.

This paper explores the study of an unknown bacterial culture. The bacterium were randomly assigned in a double blinded fashion to alleviate scientific or experimental error in determining their gram stain, morphology, arrangement, and scientific classification. There were eight unknown bacterial cultures given, with the unknown bacterial culture being one of the eight bacterial cultures. A myriad of tests and experiments were performed on the unknown bacteria in order to be able to properly classify it. One of the first experiments that was performed was the gram stain. It was determined to be gram positive. The arrangement of the unknown bacteria was also noted on the gram positive slide. The slide showed grape like

A Gram stain was used to determine the morphology of the bacteria and to confirm that the bacteria were Gram-negative. After all of the differential media were inoculated, incubated and observed, unknown bacteria number 20 was determined to be Klebsiella pneumoniae. Introduction When bacteria are obtained from the environment, one needs to perform many differential tests on the bacterial species to identify it. First, the bacteria need to be obtained in pure colonies.

Microbiology involves the study of microbes and their relevant scientific roles. The purpose of this lab report is to use the methods and techniques acquired from the microbiology course to identify an unknown bacterium. The assigned culture for this report is Unknown 28. Various laboratory techniques were performed in this process in order to draw conclusions as to what microorganism the unknown sample may contain.

Citrate positive: growth will be visible on the slant surface and the medium will be an intense Prussian blue. The alkaline carbonates and bicarbonates produced as by-products of citrate catabolism raise the pH of the medium to above 7.6, causing the bromothymol blue to change from the original green colour to

Some organisms produce an enzyme called catalase, which breaks down the toxic hydrogen peroxide (that is produced as a result of their electron transport chain) into water and oxygen gas. This test determines the presence of catalase in an organism by applying hydrogen peroxide directly to the organisms. If the tube immediately bubbles, it means that catalase was present and it turned hydrogen peroxide into water and oxygen gas. If it does not bubble, it means that catalase is absent and did not convert hydrogen peroxide into water and oxygen gas. I observed S. aureus bubble with the addition of hydrogen peroxide, meaning catalase was present. I also observed S. pyrogenes not bubble with the addition of hydrogen peroxide, meaning the organism does not produce

The purpose of this study is to identify an unknown bacterium from a mixed culture. We identified the unknown bacteria through different biochemical tests and identified the many metabolic capabilities of the bacteria. Studying and discovering unknown bacteria is very important in the health field as it may lead to new medicines and innovations for current, existing diseases as well as any upcoming diseases. The discovery of unknown bacteria is very useful in the pharmaceutical industry, as most of the products prepared are made based on these unknown organisms.