For my Final Clone Report, I choose to write about T6DL4.17. Below is the sequence of my clone: …show more content…
Comparing the BLASTx and BLASTp search results, allowed me to determine if I chose the correct ORF in the Toolbox which I believe I did as the proteins found in the BLASTp search were the same as the proteins found in the BLASTx search. The E-values for the same protein found by both the BLASTx and BLASTp searches were also the same but only the start and stop positions of the BLASTx and BLASTp alignments were different for the same protein. Overall, I determined the 5’ UTR to be G1-A28 and the ORF to be A29-G1051. What is your gene similar to? What is the ORF sequence name of the C. elegans homolog (i.e. ZC101.2)? If there is a gene name (i.e. unc-52), what is it? The name of the homolog of my gene in C. elegans is act-3 and its gene id is 179533. It is similar to ACT-1, ACT-2, and ACT-4. Its locus is T04C12.4 and it is also known as ACTin family member (act-3). What does the protein encoded by your gene do? What does it interact with? What biochemical pathway is it in? What biological function does
The closest neighboring gene to mine is named growth factor independent 1B transcription repressor (GFI1B), and it codes for Zinc finger protein Gfi-1b.
Molecular Cell Biology, 7th Edition, 2013, Lodish, Berk, Kaiser, Krieger, Bretscher. Ploegh, Amon, and Scott. W.H. Freeman and Company (ISBN-13: 978-1-4292-3413-9)
Therefore in present, looking for that CFTR gene within a region of DNA identified through linkage analysis
protein in the cell membrane. This gene disturbs the function of the chloride channels, restricting
The portions of kinesin that interact with microtubules are Loop 11, the α4 helix, and Loop 12. Previously obtained lab data has shown that mutations at the interface lead to less efficient microtubule binding and kinesin movement. Thus, we hypothesize that our newly discovered mutation will have an altered affinity to microtubules compared to wildtype. The goal of our experiments is to test this hypothesis by characterizing Kif5A protein with the P278L
Inside each and every cell in your body is a strange chemical called deoxyribonucleic acid, better known as DNA. DNA is a double-helix structure that is made up of billions of nucleotides. They are adenosine, thymine, cytosine, and guanine, abbreviated A, T, C, and G, respectively. “The information content resides in those chemical bases arranged within the interior, where A always pairs with T, and C always pairs with G” (Collins 6). These base pairs are lined up in a pattern as rungs on the DNA “ladder”. A gene is a section of base pairs in the strand of DNA. The smallest genes span about a few hundred base pairs, and the largest
Given the results of the experiment carried out it is concluded that the experimental gene ZK1225.1 is not involved with C. elegans functionality
Clones are organisms that are exact genetic copies. Cloning originally started in the year of 1885, when a German scientist named Hans Adolf Eduard Driesch began researching reproduction. The process of producing a clone began a hundred years ago. There’s many different types of clones such as, natural clones, molecular clones, organism clones and even therapeutic clones. The first organism cloned was a salamander created by Hans Adolf. Years later, Robert Briggs and Thomas Joseph King clones a frog. Then, there was a famous organism cloned by the of name Dolly, who was the first animal sheep cloned. In order to understand the creation of life one must
Sophisticated software compared these parts using existing proteins of the human genome to determine the actual proteins in the samples. They found that the Maiden's profile of
One of the main reasons I chose to research this gene is because of its association with
-A common phagemid containing several useful sequences for use in cloning that designed to simplify commonly used cloning and sequencing procedures. It has an extensive polylinker with 21 unique restriction enzyme recognition sites. It has multiple MCS and 21 unique restriction enzyme recognition sites. Also, it has High copy number ColE1-based phagemid, large and versatile polylinker in two orientations; f1 origin available in either (+) or (–) orientation, and T3 and T7 promoters for in vitro transcription of RNA
RASAL3, SASH3, PTPRC, and INPP5D. These genes are termed “hub genes” due to the high
These sequences are sourced from Subunit 1 of the Cytochrome Oxidase gene in the Mitochondria.
Scn5a is a part of the sodium channel gene family. A gene family is a group of genes that share important characteristics. In many cases, genes in a gene family share a similar sequence of nucleotides. These genes provide instructions for making proteins that have a similar structure or function. The
This being said, there is not a specific gene for a specific trait. Traits are inherited although without the environmental influence they are not expressed in an individual’s phenotype.