Identification of Macromolecules Introduction The most common macromolecules found in living organisms are lipids, carbohydrates, proteins and nucleic acids. Briefly, the meaning of macromolecules is that they normally contain two or more molecules in them and their main functions are to store energy, information and much more. Most foods are known to be combinations of macromolecules. While some of these compounds can be detected by taste tests, many cannot. Scientists then use certain tests to determine the presence of macromolecules. In doing the lab, one was able to determine the characteristics of the given solutions, containing different macromolecules, whilst doing the multiple tests. The tests performed were, …show more content…
1% starch solution | light blue | no colour change | light blue | blue/black | #9. Protein | light blue | deeper blue | dark violet | pale yellow | #10. Beer | green/blue | yellow opaque | light blue | orange/red | #11. distilled water | light blue | no colour change | light blue | pale yellow | #12. unknown #128 | light blue | Red | light blue | pale yellow | Discussion Proteins are the basis of the protoplasm (fluid living content of the cell that contains the cytoplasm and cell nucleus) and are found in all living organisms. Proteins make up the bulk of animals body’s non-skeletal structure. As enzymes, they catalyze biochemical reactions; as antibodies, they prevent the effects of invading organisms; and as hormones, they control metabolic processes (C. Bissonnette, 2011). The Biuret test was used to detect the presence of peptide bonds within proteins, and they were found present in test tube #9 (control for peptide bonds). Reducing sugars are monosaccharides that have equilibrium with the straight-chain and cyclic form, which the sugar engages in an oxidation-reduction reaction with Cu2+ (C. Bissonnette, 2011). Through this test, one is able to determine that the unknown solution #128 is a non-reducing sugar because the results show that the test turned out positive, and reducing sugars cause negative tests to occur. The iodine test is used to identify glycogen and starch. These polysaccharides combine with iodine to
The protein molecules in many foods provide the amino acid building blocks required by our own cells to produce new proteins. To determine whether a sample contains protein, a reagent called Biuret solution is used. Biuret solution contains copper ions. However, the chemical state of the copper ions in Biuret solution causes them to form a chemical complex with the peptide bonds between amino acids (when present), changing the color of the solution. Biuret solution is normally blue, but changes to pink when short peptides are present and to violet when long polypeptides are present.
Perform a series of accurate tests on biological molecules to detect the presence of carbohydrates and proteins, as well as the action of an enzyme on specific molecules.
The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes.
We decided to do the McMush Lab. The lab was designed to see the biomolecules inside an average McDonald’s Happy Meal. We decided to use a meal we have all had and to see what we were eating. Our results showed certain biomolecules.
All living things contain some form of organic macromolecules including: Lipids, proteins, carbohydrates and nucleic acids. All of these organic molecules are alike in the sense that is they are made up of bonded elements such as carbon, hydrogen, oxygen, and to smaller quantities nitrogen, phosphorus and sulfur. The macromolecules each contain large long chains of carbon and hydrogen atoms and often consists of repeating smaller molecules bonded together in a repeating pattern (polymers). To test whether a specific solid white substance is a protein is simple due to the unique chains found in the respective type of macromolecule. To test if the substance is a carbohydrate, two tests could be performed on separate samples of the object. First,
B2.Place a few crystals of copper(II)sulfate pentahydrate in a test tube. Clamp the tube and heat in a horizontal position. One of the products is copper(II)sulfate. What is the other one? This decomposition reaction is easily reversible. Add a dropperful of water to the tube when it cools. Record what happens.
A macromolecule can be any of a general selection of molecules, such as: proteins, carbohydrates, lipids, and
Therefore Iodine can be utilized to detect the presence of starch since the reaction of I2+ I- gives us I3 - . What this means is that any substance that becomes blue/black after iodine has been added to it proves that the substance has starch in it. Interestingly enough, when Iodine is added either to white rice or sweet potatoes, these two foods turn to the blue black color that confirms the presence of starch. However, If I add iodine to a banana, it only turns yellow which confirms that the banana does not contain iodine.
enzymes that will be used during this lab to test the ability of amylase to break down starch ,a
Every single living organism, such as plants and animals and also including bacteria contain the same type of large molecules that are called macromolecules. A macromolecule is a very big molecule, macromolecule are responsible for all living matter. Cells are controlled through nucleic acids , nucleic acids are a complex organic substance present in living cells. Mass spectrometry allows us to identify a simple substtance. A mass spectrometry is a technique that ionizes chemical species. A mass spectrometry can be used to analyze food and it can also be used to give answers to questions about protein like “ what “ and “ how “ . The protein hemoglobin transports oxygen to all the cells in the body. A protien hemoglobin
17. Proteins are an essential component of a healthy diet for humans (and other animals). Their most common purpose is to serve as:
First of all, the reactions that occur in the human body could not happen without the presence of macromolecules. The four macromolecules, proteins, lipids, carbohydrates and nucleic acids, make up everything in the human body. These important macromolecules could not be formed without the chemical reaction of dehydration synthesis.
There are four types of biomolecules, carbohydrates, lipids, proteins, and nucleic acids. Carbohydrates are large chains of sugar found in food and living tissues. This includes sugars, starch, and cellulose. They have the same ratio of hydrogen and oxygen that water has, 2:1. They are broken down to release energy in the animal body. Lipids are any organic compounds that are fatty acids and don’t dissolve in water but do in organic solvents. Fatty acids can be found in natural oils, waxes, and steroids. Proteins are macromolecules that do everything in the cell. They are tools and machines that make things happen. Nucleic acids are long strands of nucleotides, and function primarily in storage and transmission of genetic information. There are two types of nucleic acids, DNA and RNA. DNA is the genetic material of all cellular organisms, and RNA sends out messages from the information that is held in the DNA.
(6.2)Material and Methods in the process or exercise of measuring the starch we were used the following material and how we used them to conduct the experiment. Obtain seven tubes the material to be tested table 6.1 and then add seven to ten drops of iodine to each tube, and then record the color of the tubes contents in table 6.1
Sugars exist in solution as an equilibrium mixture of open chain and closed ring(orcyclic), strutures. sugars that can be oxidised by mild oxidising agents are called reducing sugars because oxidising agent is reduced in the reaaction. A non reducing sugar is not oxidised by oxidising agent . All common monosaccrides are reducing sugars. there are several test forr sugars which are based on composition or specific group. Hence sucrose is not a reducing agent due to bonding of oxygen with carbon to form glycosidic bonds between monosacrrides. Therefore they are not free to let go so cant be reduced , some of test includes seliwanoff 's test which distinguish between aldose and ketose sugars.Other test for sugars is benedicts test which commonly used to detect presence of