Who Killed John Lee? A Biology 111 Forensic Investigation By: Lauren Mendoza Lab Group: Liz Sanchez and Estelle Makobi Introduction The purpose of this lab is to find out who is the culprit of the murder of John Lee. The victim was found in the CCA Lab Prep room on Monday morning. There were no fingerprints found, but fiber evidence and biological evidence were collected at the scene. The reported time of death was 10:00pm Sunday night. We conducted a series of experiments to find the guilty suspect. There was a total of five suspects. Suspect one is Brandy Smith, John’s current girlfriend, she did have a strong motive to kill John; He had gone out with his ex and Brandy was very mad. Brandy’s alibi included her being at the movie theater …show more content…
In John’s text messages, Brandy texted she would “kill him”. Brandy does have a car and she also has a pet cat. Moving on, suspect two was Sergio Jones, a CCA student and the victim’s lab partner. Sergio had no clear motive to murder John, he was at the library until 8:00pm that night. Sergio does not have a car, but he does have a dog. Next is suspect 3 named Bill Apple, a CCA student and the victim’s lab partner. Bill and the victim had got into a violent argument, Bill claims the two solved the problem and they were supposed to study at the Aurora Central Library the night of the murder and the victim never showed up. Bill has car and both a cat and a dog. Suspect 4, Ann Smith, is a janitor at CCA. Ann did speak with the victim that night, she did say there was others in the building that night, but she cannot identify any one of them. She has no clear motive, she also has a cat. Lastly is suspect 5, Amanda Martinez. Amanda is the victim ‘s ex-girlfriend. John had told her that they would never …show more content…
We were using a restrictive enzyme to cut the DNA into smaller fragments. For the restriction digest we pipet 4 micrometers of enzyme mix into the bottom of each of our colored tubes making sure to use a new tip for each sample. Next, we capped the tubes and mixed the contents by flicking the tube a little bit with our fingers. After we mixed the contents, we tapped the tube to make sure all the liquid would go to the bottom of the tube. Then, we put the colored tubes in the heating blocks and we let them incubate for 35 minutes at 37 degrees Celsius. After the incubation, we removed the tubes from the heating blocks. We then got the package of 1.2% agarose FlashGel. The gel contained a chemical which allows us to see the DNA fragments under the ultra violet light. We marked where each DNA fragment would go on the gel and began to put them in the gel wells. We carefully put 5 micrometers of the crime scene samples into the wells. We had DNA from suspect 1, 2, 3, and 5. We also had the victim’s DNA and two unknown samples found at the scene. When adding the samples to the wells we used a new tip for each sample. We also made sure to wear gloves during this whole process for two reasons. First reason, the chemical used in the FlashGel is toxic and the second reason is to make sure our DNA doesn’t affect the
The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in
The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar (MSA), Blood Agar, Novobiocin, Coagulase, and DNAse (Alachi, 2007).
Make three exposures using given technical factors on a phantom knee in PA position . Include saline bags in exposures 1 and 2 to demonstrate patient soft tissue thickness.
The oxidation number of an atom of any free element is ZERO. Means to say there is only one kind of atom present, no charge.
Station one: Station one shows that when a small piece of magnesium metal is placed over the flame of the Bunsen burner, the magnesium metal ignites, giving off a bright, white light. Once the flame has extinguished itself due to the lack of fuel, the magnesium metal turned from a metallic strip to a crumbled white powder. It can be decided that station one is a combination (redox) reaction, as a new product is synthesised by the combination of two reactants. The reactants, magnesium and oxygen gas in the air bonded together to form the product of magnesium oxide.
There are four people of interest. Blood, hair, and fingerprints were all found at the scene as well
Hypothesis: The purpose of this experiment was to investigate the question will different food sources affect the level of activity of detoxification enzymes in bean beetles? The class alternate hypothesis is different food sources will affect the level of activity of the detoxification enzymes in bean beetles. The null hypothesis is the different food sources will not have any effect on the level of activity of the detoxification enzymes in bean beetles. Experimental design: The independent variables in this experiment were the types of beans (bean 1 was mung beans and bean 2 was adzuki beans) and enzymes assays used.
Introduction: Anna Garcia, age 38, was found dead in her home at 9:56 a.m., the scene was processed at 10:20 a.m. She was found near a knocked over table facedown with blood coming from somewhere on her body, most likely her head area. The EMT pronounced her dead and a investigation began on whether or not this was a crime. She recently divorced her now remarried ex-husband whom does not get along well with. The ex-husband’s wife has not been known for being nice or fond of Anna Garcia, making her a suspect.
2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.
We placed the gel into the running chamber, and then we completely covered the gel with TAE. 3 microliters of loading dye was added to each tube; this would help distinguish the enzyme from the gel. As before, we tapped the tube on the table to mix. Then we carefully added each of the four samples into their own wells. A total of 33 microliters of each sample was poured into each well. Afterwards, we attached the positive and negative electrodes to their corresponding terminals on the power supply and gel box. We turned on the power to around 80 volts and waited 45-60 minutes for the loading dye to move down the gel approximately 6-8 cm. Finally, we were able to visualize the DNA in the gel and write down the
The Case of John Lee Introduction: John Lee was murdered on a Sunday night. There are five suspects: Brandy Smith (John Lee's current girlfriend), Sergio Jones (John's lab partner), Bill Apple (John's lab partner), Ann Smith (janitor), and Amanda Maritnez (John's ex-girlfriend). The main key is to find who killed John. Everybody was in a specific place that makes them suspects, but also have motives. Brandy and John fought the night before he was killed.
2. When 2.00 g of NaOH were dissolved in 49.0 g water in a calorimeter at 24.0 ˚C, the temperature of the
The mean voltage of the battery terminals while connected to the identification resistors is presented in Figure 4 12. These samples have been pulled out from the voltage sensor of the PEB panel. The voltage decreased as expected from 12.53 to 12.5 during first 20 seconds of connection to the
Linking with Forensic science, I completed investigations which involved fragments of DNA separating. One of which was DNA separation and with 4 samples. Figuration of samples which were similar and related were identified, this was done by using an electrophoresis tray as the fragments separated, and observations were also made in the banding patterns of DNA
Scene Summary: A 55 year old white male John Doe was found dead with bullet wounds and a gun near his body. There were multiple witnesses who claim different things. On the way in there were multiple different types of footsteps on the ground. They seem to be leading towards the body. There was a fingerprint on the bookcase that could be anyone's. There was a glock handgun near the body of the victim. There seemed to be a bullet beside the gun. North of the body was a cup filled with liquid. Towards the east of the body was the same cups found near the dead man and an alcoholic drink. He appeared to have wounds on the head, chest, and wrist. There was blood on the book cases and floor. It appeared to be a struggle the way the items were placed. We found the person's wallet with his license in it, pictures, and a credit card.