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Luminol Synthesis Lab Report

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This experiment was used to demonstrate the preparation of luminol and its chemiluminescence. After luminol was synthesized by the condensation of 3-nitrophthalic acid with hydrazine, which produces 3-nitrophthalhydrazine and is reduced using sodium hydrosulfite to yield luminol, the chemical luminescent properties could be observed. Reacting luminol with potassium ferricyanide, the oxidizing agent, and hydrogen peroxide displays this chemiluminescent reaction. Amide bonds are a vital part of nature. Proteins contain peptide bonds that are positioned between amino acids and make-up the backbone of the protein structure. Also, the carbon is made a better electrophile after transfer RNA activates the carbonyl group. Amides are made when carboxylic …show more content…

This occurs when a product is generated in the excited state and then relaxes to the ground state in which a photon is emitted. When the electron relaxes, energy is released as a vibration and heat. Electrons in the same orbital are paired and must have opposite spins. There are two types of electronic states: singlet and triplet. In a singlet state, the spins of all of the electrons are paired while in a triplet state, the spins are flipped or unpaired. Phosphorescence and fluorescence occurs when electrons relax from the singlet and triplet states and emit visible light. Fluorescence emits light at a longer wavelength than it is absorbed. This process is rather fast as the light appears to be a blue-green color. Phosphorescence is very similar to that of fluorescence. However, in phosphorescence there is longer interval between the excited and ground states since the flipped spin has to flip back. This flipping of spins requires energy which leads to a less energetic triplet state compared to that of the singlet state. The longer interval in phosphorescence also sometimes results in a red color and a longer excited state period. Chemiluminescence also has other usages such as its ability to identify when blood is present. The luminol turns blue-green when the alkaline solution is exposed to

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