Spinach Photosynthesis Lab

The purpose of this lab was to see which level of light (measured in lux) made Spinacia oleracea (Spinach) leaf disks float the fastest. Our hypothesis was that an increase in light intensity will decrease the time it takes Spinacia oleracea disks to float. If light intensity is increased, then the time it takes Spinacia oleracea disks to float will be decreased. The mean for the no light (0 Lux) sample and the low light (4 x100 Lux) sample was 1200 seconds with no standard deviation because none of the disks in these two samples floated. The mean and standard deviation for the medium light (110 x100 Lux) was 902 seconds +- 84 seconds. The mean of the high light sample (410 x 100 Lux) was 692 seconds with no standard deviation because only two Spinacia oleracea disks floated so there was no need to measure the variability of the data. The final results indicated that the highest light intensity led to the quickest rise of Spinacia oleracea disks, supporting our hypothesis.

The polarity of each of the components of the spinach depends on the structure and the functional groups

Photosynthesis is a the process in which plants and other organisms to turn light into energy. To see if this is true I will test to see if spinach leaves discs can be effected by photosynthesis. I predicted that more disc would float in the light than in the dark. After writing down my hypothesis, I started the procedure. I cut up the spinach leaves into the disc and put cold water in the three petri dishes so the leaves would float in the water.

The purpose of this experiment was to determine whether spinach leaves would produce more CO2 if spinach was under blacklight, versus in the the dark. It was hypothesized that if spinach was put under blacklight, then spinach would produce less CO2 because there would be some light energy entering the plant, and that would cause the spinach to photosynthesis. This would decrease the rate of CO2 production. The data did not support this hypothesis. This investigation was valuable because it showed that respiration was still happening in the dark, which can lead to other investigation. This is also important because this showed the effects of different wavelengths of light on the rate of CO2 production in plants, and info into what might be happening

This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.

The purpose of the photosynthesis lab is to see to how fast photosynthesis occurs in a leaf in relation to different color lights. We changed the dependant variable to see how the different colors of light affects the overall time it takes for photosynthesis to take place and the leaf disc to rise. My hypothesis suggested that if the beaker had a colored lid on top of it, the light color will affect the rate of the leaf disc rise compared to the beakers with white (regular) light or no light. As it turns out, my hypothesis was supported and correct. The color of the lid did affect the rate photosynthesis occurred. The reason the leaves float to the top is because, during the photosynthesis process, oxygen bubbles form in the leaf, thereby making the leaf float.

To prepare a Ni2+ -NTA-agarose resin column, 5 mL of deionized water and 1.5 mL of Ni2+ -NTA resin slurry (Clontech, His60 Ni Superflow Resin) were added to a glass column. After thorough mixing of the column and resin settling, the water was drained from the column until it reached the top of the resin bed. 10 mL of extraction buffer (50mM NaH2PO4 (pH 7.5), 0.3 NaCl) was added to the column and half of the volume was drained. The column was kept at 4 degrees Celsius until use.

For this experiment, student acquired two clean 250-mL Erlenmeyer flasks and 75-mL glass tubes as well as labeled one tube as Tube #1 the second tube as Tube #2. The student utilized the test tubes as photosynthesis compartments. Next, student used 225mL of tap water to fill each Erlenmeyer flask previously attained. Student used 75mL of treatment solution to fill Tube #1 and Tube #2 and placed both tubes in one of the water filled 250mL Erlenmeyer flasks, the water acted as a control variable for temperature change throughout the experiment. Student acquired 2 aquatic plants from the front bench and poured water from the container, which held the plants. It was mandatory for the aquatic plants to be composed of healthy, green leaves and

Fill the container with a good-quality potting mix or garden soil mixed with compost or decomposed manure, because spinach requires a nutrient rich soil. Be sure your container has drainage holes in the bottom, and place the container on a water-catchment tray.

The effect of chloroplasts on the growth of a plant Purpose: The purpose of this experiment is to determine how different factors affect the rate of photosynthesis. Hypothesis: If the amount of chloroplast relates to photosynthesis then it is predicted that the more chloroplast present in the leaf the more oxygen bubbles produced because the function of chloroplasts is to capture energy from sunlight and use it to conduct photosynthesis, without this organelle photosynthesis would be impossible diminishing rate of photosynthesis.

The alternative hypothesis for the photosynthesis vs irradiance experiment was initially estimated to be; Ulva grown under low light will have a steeper initial slope, and a lower Pmax than Ulva grown under high light. The results have shown the initial slope (alpha) for the low light was 1.21 and for the high light was 0.81, therefore low light did have a steeper slope that Ulva grown under low light. This would be because in the low light Ulva, light is a more of a limiting factor than for the high light Ulva, therefore the low light algae have adapted to harvest the light to suit their environment. This is done by rapidly increasing or decreasing the pigment content in response to the light availability levels. By doing this the low light

The test was to see if spinach leaves could go through a short photosynthesis cycle without CO2, light, or both. This was done by using sodium bicarbonate and water to create CO2 in two of the beakers. With two beakers with CO2 and two without CO2, the effect of CO2 on photosynthesis could be observed. Because sunlight is important to photosynthesis, the other part of the experiment was to observe the difference between the spinach disks when exposed to sunlight and the spinach disks when exposed to only darkness. Because of the photosynthetic equation: 6 CO2 + 6 H2O + sunlight → C6H12O6 + 6 O2, changing the light and the CO2 would impact the outcome of the process.

The lab consisted of testing photosynthesis in leaves from different conditions. All of the conditions include: xtreme light, dark, heat, and cold. The personal condition tested by my group, was darkness. The experiment was tested by two sets of 30 leaves in the exact same solutions of 0.2% NaHCO₃ and dish detergent. The process of making the solution and preparing the experiment required: mixing 300 mL of 0.2% NaHCO₃ with two drops of dish detergent, placing the solution into a syringe containing 35 leaf discs. The finishing touches required creating pressure within the syringe to ready the leaf discs. 30 of the discs were separated, equally, into three different dishes. It was most commonly thought, that the leaf fragments would have greater

Researchers observed that two types of flowers flowered earlier than expected under red light when all others were at highest growth rate with yellow and green lights. They concluded their research by saying that both fresh and dry leaves have highest productivity under yellow light. They also mentioned that plants mass growth is actually related to many factors mainly net rate of photosynthesis and efficiency to absorb photons from light. Their conclusion also suggested that it is possible to change the light source to have it emitting more energy, but it is not possible to impose plants to make a use of that energy (Śmigielska 2014).

Light and nutrients are two key factors that are crucial for plant growth and development. The types of light required by plants are wavelengths of light that are invisible to the human eye. This light is needed to induce certain responses critical to plant development and survival. For example, the change in levels of phytochrome red light and phytochrome far red light allow plants to begin flowering, germinate, break dormancy, or senescence. Each plant species has a different photoperiod that dictates when each of these types of responses will occur depending upon the members of hours of light the plants receive. Some plants are short-day plant, meaning that they require few hours of daylight and a greater number of hours of