The Effect of Temperature on the Rate of Enzyme Activity Essay example

Catalase is an important enzyme that protects cells from oxidative damage, which hydrogen peroxide can cause. It is an incredibly efficient enzyme where one catalase molecule can convert millions of hydrogen peroxide molecules each second.

Introduction:Enzymes are made up of proteins which are produced within living cells and act as catalysts which speed up chemical reactions. They are made up of long chains of amino acids containing carbon, hydrogen, oxygen and nitrogen. Enzymes are structured to be

An Investigation on the rate of reaction of the enzyme Catalase on the substrate Hydrogen peroxide.

State the optimum pH for sucrase activity and how sucrase activity changes at more acidic and alkaline pH values. The optimum pH for sucrase activity is 6 and decrease with greater acidic and alkalline values.

Question: How does changing enzyme concentration or temperature affect the reaction time of enzyme activity?

An enzyme- substrate complex is created after a specific substrate connects with the active site. Covalent bonds in the substrate are stressed or are vulnerably oriented to attack by surrounding molecules. The enzyme can be assisted by cofactor or coenzymes in the decomposition of synthesis of the substrate or substrates. Products formed can be used to carry out various cellular functions (Dolphin and Vleck, 2015).

Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology Department, 2011).

The chemical hydrogen peroxide(H₂O₂) is broken down by the enzyme catalase. Hydrogen peroxide is a byproduct formed in cellular reactions that, if not broken down, could inflict severe damage to the cell. Catalase is an enzyme that breaks down hydrogen peroxide in to water and oxygen. How efficient and strong the enzymes reaction to break down H₂O₂ determines largely on temperature and pH level. An enzyme only functions within a set pH and temperature range. Beyond that it becomes denatured, rendering it useless. The purpose of this lab is to determine at which temperature and pH level the enzyme catalase reacts best. Catalase in chicken and beef livers will be used to do the lab because enzymes still function after death as long as they are kept refrigerated at a low temperature.

There are thousands of chemical reactions that occur in an organism that make life possible. Most of these chemical reactions occur too slowly on their own. Enzymes are protein catalysts that speed up chemical reactions in a cell. Catalysts are not changed by the reactions they control, and are not used up during the reaction. Enzymes therefore, can be used over and over again. Enzymes are large complex proteins made by the cell and allow chemical reactions to take place at the temperature of the cell. These catalysts are needed in only very small amounts because a single enzyme molecule can complete the same reaction thousands of times in one minute.

This lab served to demonstrate the catalase-catalyzed decomposition of hydrogen peroxide into water and oxygen gas under different reaction conditions. This included the investigation of the effects of enzyme concentration, temperature, substrate concentration, and presence of inhibitors on the initial reaction velocity. Because this catalase-hydrogen peroxide reaction is important in biological systems, this lab demonstrates the importance of maintaining homeostasis in cells to provide optimal conditions for these reactions. The experiments were carried out using a eudiometer and primarily concluded that reaction rate is dependent on temperature, substrate

Enzymes are protein molecules that act as catalysts for chemical reactions. Enzymes increases the rate of chemical reaction by lowering the activation energy of the reaction that it catalyses. All organisms rely on enzymes to catalyse chemical reactions and without them many reactions that would occur would proceed to slowly not being useful. Livers contain a specific enzyme called a catalase. When hydrogen peroxide (H2O2) is added to liver, a chemical reaction occurs in which the the hydrogen peroxide is broken down into oxygen gas (O2) and water (H2O). Hydrogen peroxide (H2O2) is a colorless unstable chemical that is used for oxidizing and produced as a byproduct. It is important that the catalase in the liver breaks the hydrogen peroxide

Enzymes are biological molecules that are in all organisms. They act as catalysts, which help speed up a reaction without being damaged during the process. Enzymes are extremely useful to organisms because they help the metabolism function (Berg, 2002). They are not all over the place in the body; however, they are in the cells where they make cellular digestion possible. Enzymes act upon substrates where they fit like lock and key as if they are complementary shapes (Hillis, 2012, pg. 51).

Temperature affects the rate at which substrate and enzyme molecules collide. So if temperature is below optimal level, then enzyme activity is halted, and if temperature is beyond optimal temperature, then it will denature the active site, preventing or decreasing substrate binding. Based on all the information gathered it is safe to say that there is a correlation between enzyme activity and temperature.

Almost all enzymes need specific conditions for them to function. The conditions include temperature, pH level, and concentration of salt. Enzymes have optimal conditions. If they are changed, the enzyme may denature and deactivate. If that happens, the enzyme would not be able to catalyze the reaction, and the reaction rate would decrease (Worthington 2010).

Enzymes, proteins that act as catalysts, are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes, the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol [2]. In the presence of oxygen, the removal of two hydrogen atoms oxidizes the compound catechol, as a result of the formation of water [2]. Oxygen is reduced by the addition of two hydrogen atoms, which also forms water, after catechol is