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- 1. Which of the following methods is the best way to sterilize the apparatus used in a surgical operation? A. Pasteurization B. Incineration C. Boiling in water D. Autoclaving E. Disinfect with 75% alcoholThe most versatile method of sterilizing heat-sensitive liquids isa. UV radiation b. exposure to ozone c. beta propiolactone d. filtrationThe following methods used for sterilizing an antibiotic solution. i. Dry heat sterilization ii. Microfiltration iii. Autoclaving iv. Desiccation Select one: O a. i and ii O b. i and iii O c. ii and iv O d. ii only
- 8. Match the following. A. Killing all bacteria on medical equipment via autoclaving 1. Sanitization B. Swabbing an injection site with alcohol prior to inoculation 2. Disinfection C. Application of triple antiviotic ointment to a topical wound 3. Degerming D. Use of phenol based product to clean a toilet 4. Sterilization E. Washing eating utensils in the dishwasher 5. Antisepsis 6. Chemical F. Incineration of Biohazard waste G. Filtration of heat labile liquids 7. Biological 8. Physical H. Using bacteriophages as a treatment I. Using Nitric Oxide to sterilize petri plates 9. Mechanical1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.Differentiate the following sterilization techniques: a. Autoclave b. Dry heat c. Sterile filtration
- Which of the following would be best for sterilizing a pre-sealed case of plastic medical supplies? A. Autoclave B. Ionization Radiation C. Sonication D. Nonionizing Radiation10. Which one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? a. Gradient diffusion method b. Agar macrodilution method c. Disk diffusion method d. Broth microdilution method 11. Most ATP molecules are produced during which phase of aerobia respiration? a. Fermentation b. Krebs cycle c. Glycolysis d. Electron transport chain 12. The goal of disinfection is to eliminate ____ whereas the goal of sterile technique is to exclude ____ a. bacteria , bacteria and viruses b. pathogens, all microorganisms c. nonpathogenspathogens d. all microorganisms, pathogens 13. Which one of the following statements about archaea is false? Select one: a. The cell walls of archaea contain a thicker layer of peptidoglycan than the cell walls of bacteria. b. Some archaea live in extremely hot environments. c. Archaea are more closely related to eukaryotes than they are to bacteria. d.…1. Given the following common laboratory materials, which of the three methods of heat sterilization do you think is the most appropriate and practical to use in sterilizing them Why do you think so ? Method of heat sterilization Reason a. Empty flasks b. L-rod (glass rod) c. 10 ml glass pipet d. Wire needles e. Antibiotic solution
- 1. Which of the following manufacturing practices to maintain probiotic label claims regarding bacteria counts can negatively impact patient health? A. Freeze-drying B. Overstocking C. Refrigerated shipping D. Surveillance cultures 2. Which of the following is a prebiotic? A. Bifidobacterium bifidum B. Enterococcus faecium C. Oligofructose D. Saccharomyces boulardii 3. Which factor is LEAST important when selecting a probiotic? A. Cost of product B. Manufacturer quality control/testing C. Number of bacterial strains D. Presence of prebiotics1. Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0 ml B. spreading of the inoculum is done using a disinfected glass L-rod C. A pre-solidified medium is required prior to inoculation of the sample. D. A and B 2. Plates inoculated with bacteria are inverted during incubation to: A. prevent contamination B. limit the colony growth C. A and B D. A and C E. All of the above F. None of the aboveWhich of the following methods is most sensitive for identifyingdifferent strains of a microbe?a. microscopic examination b. hemolysis on blood agar c. DNA analysis d. agglutination test
