1. Describe the difference in the bands for the PCR and RT-PCR from the CD4+ T cells (ie. Make an observation of the result) 2.Explain the difference in results for the PCR and the RT-PCR from the CD4+ T cells (ie. propose a conclusion)
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1. Describe the difference in the bands for the PCR and RT-PCR from the CD4+ T cells (ie. Make an
observation of the result)
2.Explain the difference in results for the PCR and the RT-PCR from the CD4+ T cells (ie. propose a
conclusion)
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- The image below shows the general structure of a gene on a chromosome. The arrows above and below the chromosome indicate the binding positions of potential forward (F) and reverse (R) PCR primers. Select two primers from the list below that would exclusively amplify exon 3 in a PCR reaction. ut of Intron 1 Intron 2 +1 Poly-A signal ATG TAG F1 Exon 1 F4 Exon 2 F6 Exon 3 R6 R4 R3 R2 R1 Promoter Select all that apply: cross out Da. F1 cross out Ob. F2 cross out Oc. F3 cross out O d. F4 cross out O e. F5 cross out f. F6 cross out g. R1 cross out Oh. R2 cross out OL R3 cross out O. R4 cross out k. R5 cross out R6 TIThe following statements are true about common gene cloning procedures except: -DNA plasmids can help move around genes into other cells - Restriction enzymes are very important for cutting up and linking chunks of DNA -We make it so that genes from plants or animals are expressed in bacteria so the products can be harvested - DNA plasmids are chunks of chromosomal DNA used for cloningRegarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genome
- The genomic DNA of a bacterial cell is NOT destroyed by the cell’s own restriction enzymes because Choose an answer from below: the bacterial DNA is too small to contain the recognition sequence for the enzymes. the restriction sites are occupied by histones. the genome is protected by the nuclear membrane. the restriction recognition sequences in the genome are protected by a microRNA. none of the aboveWhich of the following would you need to clone a human gene? Choose all that apply A restriction enzyme Human DNA A donor goat nucleus An enucleated egg A surrogate mother DNA ligase A plasmid vector (or other type of DNA vector, e.g. phage, BAC)The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes. 1. T7 RNA polymerase 2. Reverse transcriptase 3. RNaseH
- Which of the following is a unique attribute of CRISPR/Cas9 compared to restriction enzymes Group of answer choices it can be targeted to any specific region of the genome as long as there is an adjacent PAM motif it was first discovered in bacterial cells it can cut double-stranded DNA sequences it can be used for molecular cloning experimentsIn biotechnology procedures, a(n) ____ is a nucleic acid fragment that is used to search for and identify a sequence of interest. vector probe library antibody plasmidCRISPR/Cas9 can be used in genome editing. Among the following statements, which one is correct? The CRISPR and the Cas9 parts both recognise the DNA target sequence and then recruit an endonuclease for cutting it The Cas9 part recognises the DNA target sequence and the CRISPR part cuts it The CRISPR and the Cas9 parts both recognise and cut the DNA target sequence. The CRISPR part recognises the DNA target sequence and Cas9 cuts it.
- During nucleic acid hybridization, the probe is labelled for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplificationCRISPR One of the more recent advances in biotechnology is the development of the CRISPR gene modification tool. Match the following descriptions the the key players in this technology. NOTE: If you want to change your selection, you'll need to delete the one you already chose. After you delete it, the list of choices will pop back up and you can make a different choice. CRISPR technology Specialized stretches of DNA with nucleotide repeats and Cas9 protein spacers Enzyme that cuts DNA crRNA Guides the enzyme to the target site CRISPR DNA Adapted from the natural defense mechanism of bacteria and archaeaThe genomic DNA of a bacterial cell is not destroyed by the cell's own restriction enzymes because the bacterial DNA is too small to contain the recognition sequence for the enzymes. the restriction sites are occupied by histones. the genome is protected by the nuclear membrane. the pH in the bacterial cell does not allow the restriction enzymes to function. None of the above