1. You are working on an experiment with a newly discovered enzyme. This enzyme works most efficiently at a pH of 7.70. You make 250 mL of a 150 mM phosphate buffer, pH 7.70. (pk = 6.82). a. What are the concentrations of the weak acid and conjugate base forms of this phosphate buffer when you start the experiment? In the process of the enzyme catalyzed reaction, 25 mM acid (H+) is produced. What is the pH of the solution after the reaction is complete? Assume no change in volume by production of the acid.
1. You are working on an experiment with a newly discovered enzyme. This enzyme works most efficiently at a pH of 7.70. You make 250 mL of a 150 mM phosphate buffer, pH 7.70. (pk = 6.82). a. What are the concentrations of the weak acid and conjugate base forms of this phosphate buffer when you start the experiment? In the process of the enzyme catalyzed reaction, 25 mM acid (H+) is produced. What is the pH of the solution after the reaction is complete? Assume no change in volume by production of the acid.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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