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- 23. Design the forward, reverse, and internal primers to the gene below and mutate the bolded/underlined histidine (CAC) to an alanine (GCC). Primers are usually 18-25 nucleotides long, but only make primers 9 nucleotides long to save work. 5'-ATGGATCGATACGGACGAAATCACCTACCGAATCAGGCGTGACGTGA-3' External Forward: External Reverse: Internal Forward: Internal Reverse:I still do not understand why the number of coding regions are 6 based on the R-loop.BM4_DNA AND PROTEIN S X /1FAIPQLSDP_g5B-629FSHNpGnTMIEppLS4A71zBd4vcUBqNUILubXONw/formResponse 4. What is the nitrogen base pair of Adenine in transcription? O Cytosine O Uracil O guanine O thymine 5. The central dogma of Molecular Biology states that There are four nitrogen bases in DNA, two purines (adenine and guanine) and two pyrimidines (cytosine and thymine). Which process is not included in the central dogma? duplication transcription translation O translocation Leadple
- +1 1 2 4 6. 7 8. 9. Transcriptional stop sequence ТАTА box ATG ТА AUG UAA Here is a diagram of a human gene in the genome. The bar above it is indicating different regions of the gene sequence. 7. Which region or regions contain the sequences that direct splicing? - 8. In which region or regions could a single base pair insertion cause a frameshift mutation (but not because of changed splicing)? - 9. In which region or regions might you find sequences that control the amount of transcription of this gene? + 10. In which region or regions could a single base pair deletion cause the mRNA to be one base pair shorter? - 11. Which region or regions usually contain a sequence that controls the secretion of the protein e product? eYou have the following DNA coding sequence of a wild-type allele: 5’-ATG TTC CAG CTA GAT GAT ATG CTG GTA ATT GGG GAA CGC GCG CGG TAA-3’ 1. For the second, third, fourth, and fifth codons, write all possible anticodon sequences (left-to-right, 5’- 3’), including anticodons with wobble and inosine.Fig 3.16 EcoRI SacI KpnI |ampR Aval Xmal Smal lacZ BamHI Xbal SalI Accl HincII PstI Sphl HindIII Bam H1 Bam H1 Bam H1 1 2 3 4 The ends of the double-stranded DNA fragment above are blunt ends. The directionality of genes contained within the fragment is from left to right (arrow). After digestion by BamH1, which fragment can be inserted into the vector cut with BamH1 and Sma 1 maintaining the same directionality as the lacz promoter (green segment with arrow on vector)? 3 O 4 O1 2
- Hơ many mismatches would you expect to find between this sequence and the reference sequence? I know the answer is 3, can you please explain why?5² | 1. Draw on the first 4 RNA primers and label the 3'OH ends 2. Identify what will be leading and lagging strands 5'31 If a point substitution mutation changes the sequence 5'ATGAAA3' to 5'ACGAAA3' in the MIDDLE of the coding region, what is the most likely Second letter result? Phe UCU UUCJ UAU Tyr UUT UGU 1 UGC UAA Stop UGA Stop UAG Stop UGG Trp UC UACJ Cys Ser UUA UCA Leu UUG UCG CU CUC CUA CUG CCU CAU) CGU His CC C CAC) Leu CA Pro Arg CAA Gln CAG CGA CGG AUU AAU AAC Asn AGC Thr AAA ACU AGU AUC lle ACC Ser AUA AUG Met ACG ACA AGA 1 AAG Lys AGG Arg GUU GUC Val GCU GAU] Asp GAC) Ala GGU GGC GCC GUA GCA GAA1 GGA Gly Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a no protein is made no effect protein has altered amino acid sequence protein is not made because of frameshift mutation Third letter First letter
- What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome c. Proteome d. Metabolome e. FluxomeMuscle cells differ from bone cells because ________. a. they carry different genes b. they use different genes c. both a and bA chromosome contains many different genes that are transcribed into different ___ . a. proteins b. polypeptides c. RNAs d. a and b
