12. Assume that spherical particles with diameter=100 µm and density=1.1 g/cm³ have sedimentation velocity of 1 cm/min in a solution with density=1 g/cm³. What is the sedimentation velocity of spherical particles with diameter=50 µm and density=1.3 g/cm3 in the same conditions? SOLUTION: 13. Which method, Rate Zonal Centrifugation or Isopycnic Centrifugation, is better suited to separate RNAS from proteins? Briefly explain why. SOLUTION:
Q: Present an overview of the manner in which chromatin can beremodeled. Describe the manner in which t...
A: Gene regulation is the process of regulating the expression of specific genes at a time from among a...
Q: Direction: Complete the Venn Diagram. In the outer circles, write the difference between negative an...
A: Negative feedback: A control system that helps the body maintain homeostasis by sending a signal to...
Q: Brown fat metabolism generates about 10x more heat than white fat metabolism True False
A: Introduction: Brown fat is also called brown adipose tissue. It is found in many mammals. Brown fat ...
Q: If the true inheritance pattern was a normal Mendelian dihybrid cross, it would be more likely to re...
A: * Mendels dihybrid cross : *Dihybrid cross means crossing between two different genes that has two...
Q: If you are a strong taster, how many bands would you expect on the gel after electrophroesis? O1 O 2...
A: Electrophoresis It is defined as the method through which DNA, RNA, or protein is separated on the b...
Q: Drinking too much alcohol is bad for your health. One unpleasant effect of drinking too much alcohol...
A: Vasopressin is another name for antidiuretic hormone (ADH). It is a naturally occurring hormone that...
Q: QUESTION 1 Glucocorticoids: promote the immune response promote the release of fatty acids increase ...
A: Glucocorticoids (GCs) are steroid hormones widely used for the treatment of inflammation, autoimmune...
Q: Insulin is a protein secreted by the beta cells of the pancreas into the blood. Which of the followi...
A: Insulin is a hormone which is released by Beta cells of pancreas. And in the given options,option 1 ...
Q: Describe how one cell communicates with another cell. What does this communication actually do? In o...
A:
Q: Fulfill the epidemiologic triad as to the host, agent, possible vectors, and environment of the Avia...
A: Avian means birds, so flu related to birds is Avian flu. The symptoms include the symptoms of conve...
Q: In which part of the nephron is water and sodium chloride reabsorption due to hormonal signaling?
A: The nephron is the small but most important unit of a kidney. The components of nephron include: re...
Q: QUESTION 1 Bald Lamprey bass Antelope eagle Alligator Sea Fur Feathers Gizzard ng, Jaws Based on the...
A: Phylogenetic tree: A phylogenetic tree is a graphic that shows how creatures have evolved over time....
Q: 1. iven what we understand about social structure, which type of social structure is generally ass...
A: 1.One male(Polygyny) social structure is the type of social system in which a single male interacts ...
Q: Where do spindle fibers attach on a chromosome? O cell plate O meristem centromere
A: The chromosomes can be described as the thread like structures that can be found in the nucleus of t...
Q: Which of the following are MOST likely to cause a person to make ketone bodies (check all that apply...
A: Introduction :- Ketone bodies are water-soluble molecules containing the ketone groups generated by ...
Q: at are the types of hair
A:
Q: Where on the tree did the amniotic egg evolve? Group of answer choices C A D E B
A: The given tree depicts the evolution of the different chordates as vertebrates, then jawed vertebrat...
Q: Maria just got into a car accident and now cannot feel pain on the right side of her lower body. She...
A: Maria just got into a car accident and now cannot feel pain on the right side of her lower body. She...
Q: define a single nucleotide polymorphism
A: Introduction:- Genetic polymorphism is the inheritance of a trait controlled by a single genetic loc...
Q: Do individual organisms survive exposure to a toxic chemical because they are “mutated” by the chemi...
A: A gene is the essential physical and functional unit of heredity. They are comprised of DNA (deoxyri...
Q: C) A) B) D) E) Directions: For cach of the following species above identify whether they are Haplorr...
A: Strepsirhines: B and C. Haplorrines: A, D, E. Platarrhines: A and B Catarrhines: D and E
Q: Rationalize on why a leaky heart valve might injure the heart overtime.
A: Leaky Heart Valve: When the heart beats, four valves of the heart open and close. They regulate blo...
Q: a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a ...
A: The staining is a very important process in microbiology that is used to identify specific microorga...
Q: What are the types of plant geotropisms? Why do the stem and the roots present opposite geotropisms?
A: Introduction: Gravitropism ,also known as geotropism, is a coordinated process of differential growt...
Q: 1-true tissues or not- who does not have? 2- radiate/bilateria- who for sure is in radiate? 3- diplo...
A: Kingdom Animalia / metazoa The kingdom Animalia is classified into Two Sub Kingdoms , they are - P...
Q: B: Lambda DNA/Hindlll Marker, 2 GeneRuler" 1 kb DNA Ladder O'GeneRuler" 1 kb DNA Ladder, ready-to-us...
A:
Q: Which of the following is a risk factor for the development of cancer, but not also for diabetes and...
A: Risk factors for development of cancer : Tobacco Exposure to radiation Specific Chemicals Older age...
Q: What phase is the cell in #5 in? O Prophase O Metaphase O Interphase Anaphase
A: *The cell division mitosis will have four stages Prophase Metaphase Anaphase Telophase * During ...
Q: Gregor Mendel followed specific steps when breeding pea plants to determine the underlying cause and...
A: Introduction: Gregor Johann Mendel, an Austrian Monk, discovered the principles of heredity through ...
Q: Identify the factors that contribute to total peripheral resistance.
A: Introduction :- The resistance to blood flow offered by all of the systemic vasculature, excluding t...
Q: Did an Error Cleaning Mouse Cages Alert Us to the Dangers of Bisphenol A?
A: BPA is quite dangerous for human beings. BPA has a tendency to go through the bodies of humans in a ...
Q: What are the five primary causes of biodiversity loss? Give one specific example of each
A: Biodiversity loss is caused by five primary major factors: habitat loss: An example of such factor ...
Q: Place the steps to the scientific method in the correct order 1 V [ Choose ] Make predictions Commun...
A: *scientific method is a method for acquiring the knowledge by development of science . *It involves ...
Q: Associate the biochemical reaction in TSI agar to certain genera or species. Reaction Possible Organ...
A: The triple sugar iron test ( TSI ) in microbiology it is a test which use for ability to test micr...
Q: What is the mode of action of bacitracin? How does this influence the types of bacteria it can be u...
A: Introduction:- Microorganisms such as bacteria, fungus, and protozoans are killed or inhibited by an...
Q: Vertebrates have a complete digestive system, which means that their digestive tract has two opening...
A: *All invertebrates do not have complete digestive system * The complete digestive system forms from...
Q: Question 5 Voles and lemming are found throughout the Arctic. Which of the following statements is c...
A: Voles and lemmings These are widely spread across the Holarctic. They are located in temperate North...
Q: How do fertilization and zygote formation occur in these plants? Do these processes depend on water?
A: Introduction In this question we will discuss about how fertilization and zygote formation occur in ...
Q: 9. In cocker spaniels, the following genotypes and phenotypes are found: AABB = white, A_bb = red, a...
A: According to our guideline we can answer only one question and up to maximum 3 subparts. The questio...
Q: In dogs, a dominant gene (1) determines white coat, and a recessive gene (i) gives colored coat. In ...
A: The phenotypic ratio would be 12:3:1. The genetic interaction is recessive epistasis.
Q: Which is considered a true breeding plant? A. AABBcc B. AaBbCc C. AABBCc D. AaBBCC
A: The genotype of an organism is the genetic composition. The diploid organisms have two sets of chrom...
Q: Can you tell me about the contribution of Don Francis in the society's knowledge about HIV?
A: AIDS is an Acquired Immune disorder syndrome that is caused by HIV (Human Immuno deficiency virus). ...
Q: In the phosphorus cycle, all phosphorus originates from which of the following inaccessible reservoi...
A: Phosphorus is an important component of cell membrane, nucleic acids and ATP which is the major ener...
Q: Based on the character matrix in the picture, draw a Venn diagram. Start with the character that is ...
A: Venn diagram Venn diagram is a circular or rectangular diagram which illustrates the relationship b...
Q: All of the following can be associated with human blood type except A. Multiple alleles B. Codominan...
A: Meiosis It is the type of cell division that results in 4 daughter cells, each with half the number ...
Q: The “kingdom” concept is considered out-of-date but most continue to use it in a revised manner. Wha...
A: Linnaeus's hierarchical system was developed by Carolus Linnaeus in 1735 and he is called a father o...
Q: With the cladogram below as a basis, answer the following questions in the space provided. 6. Write...
A: Cladogram is a diagrammatic illustration in which relationship of taxons with each other and with an...
Q: what is the purpose of the nucleous ? - define diploid
A: In biology, a cell is a membrane-bound unit that holds the basic molecules of life and is made up of...
Q: Fulfill the epidemiologic triad as to the host, agent, possible vectors, and environment of the Poli...
A: Poliovirus is the causative agent of polyomylitis, is a human enterovirus and member of the family o...
Q: What is the BEST explanation for why DNA replication is discontinuous at the lagging strand? А. DNA ...
A: Replication process synthesizes DNA by the action of DNA polymerase enzyme.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- 2. For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. How much of the diluent must be added to tube 2 if 200uL Red cell suspension is added? Show pertinent solutions. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions.Show the calculations required to make up 250mlof a stock solution of this chemical that will then be at the working concentration when 1 volume of this solution is added to 10 volumes of cell culture medium.When combining 1ml of lysosome solution with a concentration of 5mg/ml and 1ml of water what is the final concentration of the lysosome solution after adding 8.0 of a biuret reagent to the solution 2.5mg/ml 1mg/ml 0.5mg/ml 5 mg/ml
- a)Determine the amount of X (in gram) in order to prepare 100 mL of 50 mM stock solution of X. Show your work. (MW of X= 225) b)Determine the volume required (mL) from the stock solution X in (i) to prepare 500 mL of MS medium containing 5 mg/L X. Show your work.Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.Serial dilution = previous dilution x dilution 11 mL [2] 3 mL Colony count: 100 0.1 mL sample [1] 9.9 mL 5 mL 0.1 ml. Cokony count Dilution: 10-1 10-3 [3] 150 Dilution factor (DF): [4] [5] [6] Compute for the CFU/mL of the sample. Show your solution and express your final answer to 2 significant figures.
- 1- Are the following changes in conditions likely to increase or decrease retention time? a. faster flow rate b. longer column c. longer connector between column and detector d. higher analyte concentration e. higher temperature f. larger sample size g. using a less polar column while doing a reverse-phase separation h. using a less polar mobile phase while doing a reverse-phase separation 2- In what order will the following substances elute? • In reverse-phase liquid chromatography: a. butylamine, 2-butylene, ethylamine, ethylene b. benzene, chlorophenol, phenol, trichlorophenol • In gas chromatography with a polar column: c. butanol, methanol, water • In gas chromatography with a nonpolar column: d. butanol, methanol, water • In ion chromatography: e. bromide, fluoride, sulfide1. (2.5 pts) A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as diagrammed below. Each dilution tube contained 400 ul of diluent and 100 ul was transferred into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes. a. What is the dilution between each tube shown in the diagram below? Express your answer as a ratio. b. What is the total dilution of tube number 5? Express your answer as a ratio. c. What is the concentration of viable bacteria in the original culture? Express your answer using scientific notation and the units CFU/ml. d. What is the concentration of viable bacteria in tube number 2? Express your answer using the units CFU/ml. e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would you expect to see after the plate was incubated? 1 2 3 5 423 80 13 Number of coloniesIf the volume of a Staphylococcus aureus cell is estimated at 0.5 μm3, how many cells could be accommodated, in principle, in 5 mL of saturated culture? (1 mL = 1 cm3). Show your calculations.
- Consider the solutions shown in the containers below. The composition of each solution is given in the image. 0.15 Mlactose isotonic hypotonic hypertonic crenation hemolysis no change A 0.28 M Urea B Fill the blank with the appropriate term from the list below. 0.16 MKCI The tonicity of the solution in container C isA culture of S. cerevisea has an overnight OD of 2.3 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies. How much of the 2.3 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ulImagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?