16. An experiment was conducted for an enzyme catalyzed reaction and data was gathered in a table. In the experiment, the same amount of enzyme was used for each trial. The amount of substrate added, and the pH, were adjusted to see the impacts of concentration and pH on the ability for the enzyme to catalyze the reaction. Using the data table provided from the experiment (listed below), answer the following questions Amount of Substrate a. 2.0g 4.0g 6.0g 8.0g pH 3 18 24 55 55 pH 6 10 19 27 27 pH 9 35 78 92 92 pH 11 20 32 47 47 pH 13 3 10 18 18 Yield of products (number of products produced in the reaction) Looking at the data table, what was the optimum pH for this reaction? (What pH provided the highest yield of products?) b. What pH value generated the lowest yield of products? C. Briefly explain below, why was there no increase in the amount of product formed with 8.0g of substrate as compared to 6.0g of substrate? (If you are supplying more substrate, why are we not seeing more product?)

16. An experiment was conducted for an enzyme catalyzed reaction and data was gathered in a table. In the experiment, the same amount of enzyme was used for each trial. The amount of substrate added, and the pH, were adjusted to see the impacts of concentration and pH on the ability for the enzyme to catalyze the reaction. Using the data table provided from the experiment (listed below), answer the following questions Amount of Substrate a. 2.0g 4.0g 6.0g 8.0g pH 3 18 24 55 55 pH 6 10 19 27 27 pH 9 35 78 92 92 pH 11 20 32 47 47 pH 13 3 10 18 18 Yield of products (number of products produced in the reaction) Looking at the data table, what was the optimum pH for this reaction? (What pH provided the highest yield of products?) b. What pH value generated the lowest yield of products? C. Briefly explain below, why was there no increase in the amount of product formed with 8.0g of substrate as compared to 6.0g of substrate? (If you are supplying more substrate, why are we not seeing more product?)

Chemistry for Today: General, Organic, and Biochemistry

9th Edition

ISBN:9781305960060

Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen

Publisher:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen

Chapter20: Enzymes

Section: Chapter Questions

Problem 20.53E

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Similar questions

Most human enzymes function best in the temperature range of: a. 0C10C b. 10C25C c. 25C40C d. 40C55C
List two ways that enzyme catalysis of a reaction is superior to normal conditions.
Compare the lock-and-key and induced-fit models for substrate binding to an enzyme.
. Describe the lock-and-key model for enzymes. Why are the shapes of the enzyme and its substrate important in this model? What does it mean to say that an enzyme is inhibited by a particular molecule? What happens if this inhibition is irreversible? Can you think of a situation in which it might be advantageous to be able to inhibit an enzyme?
Describe the difference between graphs showing temperature versus reaction rate for an enzyme-catalyzed reaction and an uncatalyzed reaction.
True or False: Enzymes lower the activation energy for a reaction. I. Genetic message are decoded and used to build protein. II. Identical copies of DNA are made so that genetic information can be preserved and handed down to offspring. II. Genetic messages are read and carried out of the cell nucleus. Which describes the correct order of transferring genetic information in biological system? In base solution, an amino acid is amino acid is and exists primarily as an anion, while in acid solution, an and exists primarily as a cation. protonated, deprotonated deprotonated, protonated zwitterion, protonated zwitterion, deprotonated Structure IV is an example of 手美 OH но HO -O- OH но -H Но Но -H но -H но -- но- -H -O H -OH H FOH H- -OH -OH- H OH OH но OH но но он H. Он II II IV V Which is NOT correctly paired? fibrous protein: spherical alpha helix protein: right-handed coil beta pleated sheet: fully extended peptide chain globular protein: soluble in water
hydrogen peroxide was added to a piece of potatoes contain the enzyme, catalyze, hydrogen peroxide was added to the potatoes and bubbles appeared to the presence of oxygen gas, if the hydrogen peroxide was removed and re-added to the potatoes, would bubbling occur again? explain why or why not.
1. What is an enzyme? 2. What is the function of an enzyme? 3. Which graph would represent enzyme activity Energy |CO, + H,O HCO3 +HT Progress of Reaction 4. What are the six classes of enzyme? 5. Explain a competitive inhibitor In the induced-fit model, what happens to the shape of the enzyme when the substrate binds? A. stays the same B. adapts to the shape of the substrate 35) An irreversible inhibitor is one that A) forms hydrogen bonds with the substrate. B) binds to the enzyme with hydrophobic interactions. C) reacts covalently with the substrate. D) binds covalently to the enzyme active site. E) reacts covalently with a cofactor. 47) Metal ions such as Zn²+ and Fe3+ are often needed by enzymes as A) isozymes. B) allosteres. C) inhibitors. D) cofactors. E) substrates. 8) The optimum pH for the activity of trypsin is about A) 2.0 B) 4.0 C) 6.5 D) 7.4 E) 8.0 3) An enzyme that converts a cis double bond to a trans double bond is classified as a(n). A) oxidoreductase B) transferase…
Enzymes and substrates have what is known as the lock-and-key mechanism. How so?
II.Match each item with the correct statement below. a. Le Chatelier's Principle d. substrate b.electrochemistry c. electrolysis e. Gibs Free Energy f. Collision Theory 41. Define as G=H-TS 42. It states that particles collide for chemical reactions to occur. 43. It specifically binds on an active site to bound to the enzyme. 44. A branch of chemistry that deals with the intercharge between chemical energy and electrical enery. 45. It implores that stress may cause the equilibrium to shift toward the direction in which the stressis relieved 46. It employs the use of electric current to bring chemical change .
What parameters associated with enzyme activity are related to the Arrhenius equation? How can those parameters be plot to extract a relationship between them?
What should be taken in consideration when designing an enzyme kinetics experiment so that the reaction will not be too slow or too fast? M - amount of substrate B - amount of enzyme |- temperature O - pH