4. Suppose the data shown below are obtained for an enzyme catalyzed reaction. [S] (MM) v (mmol/ml/min) 0.1 3.33 0.2 0.5 0.8 1.0 2.0 5.0 7.14 8.0 8.3 9.09 a) Plot the data and determine Km and Vmax (please include the plot). b) Assuming the enzyme was used in these reactions at a concentration of 106 M, then calculate the turnover number for the enzyme.
4. Suppose the data shown below are obtained for an enzyme catalyzed reaction. [S] (MM) v (mmol/ml/min) 0.1 3.33 0.2 0.5 0.8 1.0 2.0 5.0 7.14 8.0 8.3 9.09 a) Plot the data and determine Km and Vmax (please include the plot). b) Assuming the enzyme was used in these reactions at a concentration of 106 M, then calculate the turnover number for the enzyme.
Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter11: Chemical Kinetics: Rates Of Reactions
Section: Chapter Questions
Problem 83QRT
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![4. Suppose the data shown below are obtained for an enzyme catalyzed reaction.
[S] (MM)
v (mmol/ml/min)
0.1
3.33
0.2
5.0
0.5
7.14
0.8
8.0
1.0
8.3
2.0
9.09
a) Plot the data and determine Km and Vmax (please include the plot).
b) Assuming the enzyme was used in these reactions at a concentration of 10-6 M, then calculate the
turnover number for the enzyme.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F7a918600-ca67-45f5-9085-6eb6f917baf2%2F5a81ef5c-3c2f-4617-8cbc-7fbbe3890993%2Fcfqzs92_processed.png&w=3840&q=75)
Transcribed Image Text:4. Suppose the data shown below are obtained for an enzyme catalyzed reaction.
[S] (MM)
v (mmol/ml/min)
0.1
3.33
0.2
5.0
0.5
7.14
0.8
8.0
1.0
8.3
2.0
9.09
a) Plot the data and determine Km and Vmax (please include the plot).
b) Assuming the enzyme was used in these reactions at a concentration of 10-6 M, then calculate the
turnover number for the enzyme.
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