5. Would ninhydrin test be useful and reliable for showing the presence or absence of proteins? Explain. 6. From the data in table C, which protein can bind with heavy metals and may find use against heavy metal poisoning?
Q: III. True or False a. Large proteins travel/migrate faster in both gel filtration chromatography…
A: Different techniques used in the separation and purification of the biomolecules are, Chromatography…
Q: 1. Why must the solution to be tested with ninhydrin be neutral?
A: Since you have asked multiple question, we will solve first question for you. If you want any…
Q: A ligand binds more tightly to the folded state (N) of a protein than to the unfolded state (U).…
A: The formation of a ligand-protein complex is an exothermic process with a ΔH equal to the binding…
Q: 4. a. Draw the structure of the dipeptide Valine-Serine at its isoelectric point. The side chain for…
A: Amino acids are building block of protein and peptides. There are 20 naturally occurring amino acid,…
Q: Rationalize the following observations.(a) Serine is the amino acid residue that can be replaced…
A: (a) Serine is the amino acid residue that can be replaced with the least effect on protein structure…
Q: Why do we need to study the four levels of protein structure? Cite its practical use to our…
A: Protein structure is a complicated structure made of many amino acids linked together by peptide…
Q: . If catalase is tested with the biuret reagent, what would be the ideal result? Why?
A: To measure total protein present in our body total protein lab test is to be done. The growth…
Q: 2 a. You are trying to purify protein C from a mixture of proteins noted in the above Table. If you…
A: Chromatography is biochemical separation method for organic molecules or solutes of a compound…
Q: Define and state there functions a. Fibrous protein b. Globular proteins
A: Protein is a macronutrient essential in diet. It is composed of primary, secondary, tertiary and…
Q: As you have seen, the protein assay relies upon the ability of proteins to bind the dye. Some…
A: Proteins are made up of amino acids attached together via peptide bonds. Proteins are composed of…
Q: 4. The table below describes a procedure in preparing standard solutions of a protein sample with…
A: Proteins are chain of amino acids linked by the peptide bonds, a covalent bond. Its concentration…
Q: The free energy of folding of a protein is -17kJ/mol. What tenoerature (C) do you have to heat the…
A: Protein folding is related to protein stability that is the thermodynamic stability, not the…
Q: 8. One amino acid contains a guanidino group. What is the approximate pka of the weak base in the…
A: Amino acids are organic compound having two functional group namely carboxyl and amino. An amino…
Q: 2. Suggest a reason why amino acids are usually more soluble at extremes of pH than they are at…
A: Amino acids are the smallest units that makeup peptides and proteins inside the cell. Amino acids…
Q: One tenth mL of a protein solution was diluted with 2.5 mL of water. The A280 of the diluted…
A: Protein concentration by using absorbance spectroscopy at 280 nm. The proteins absorb maximum light…
Q: 1. What is the major protein found in egg white? In milk? 2. What caused the results you observed?…
A: Vinegar when added to milk causes an irreversible reaction leading to curd (solid) and whey (liquid)…
Q: 1. The use of natural proteins as drugs requires compliance with certain storage and use conditions,…
A: Protein: Polymer of amino acid joined together through peptide bond removing water in a process…
Q: Calculate the molecular weight of protein in sample A. Given the protein migration distance is 26 mm…
A: SDS PAGE is a technique used to separate proteins based on their size. The proteins are first…
Q: fter processing all of your protein samples in the laboratory, you are left with one sample to…
A: The mechanical process that is used for the separation of particles in a solution includes…
Q: Under what pH conditions can a protein not bind to the beads in a column? pH = -pKa pH =…
A: Amino acids are the building blocks of proteins. All of the proteins found inside the cell are made…
Q: The rate of migration of a protein through an SDS-poly-acrylamide gel is NOT influenced by A.…
A: The polyacrylamide gel SDS is a kind of analytical technique used to seperate proteins. SDS page is…
Q: What are the advantages and disadvantages of using the Ehlrich test for proteins Suggest how the…
A: Introduction: Ehlrich test is a biochemical test that is performed to detect a particular amino…
Q: 1. In the preparation of the standard curve for protein analysis, 50 mn BSA (bovine serum albumin)…
A: Given values: 50 mg of BSA was dissolved in 5 ml of water. So, the concentration of BSA in the…
Q: 4. A particular amino acld inside or the outside of the folded protein? Briefly explain. on the
A: Amino acid in nature has the same fundamental structure. It consists of a carbon atom, the alpha (α)…
Q: List some important types of molecular interactions involved in recognition and binding of…
A: Polysaccharides, also called as glycan are the abundant carbohydrates found in food. They are…
Q: 1. How many different tripeptides are possible? How many carboxyl terminals of polypeptide chains…
A: Tripeptides are composed of three amino acids. There are 20 naturally occurring amino acids . A…
Q: Calculate the approximate MW of a protein with 341 amino acid residues and express it ing/mol and…
A: Given Values: Number of residues in the protein = 341 aa Average molecular weight of an amino acid =…
Q: Which of the following might cause the denaturation of a protein? Select all that apply. A.…
A: PROTEIN STRUCTURE:- Protein structure is a 3 D Structure which forms 4 structures: 1)PRIMARY…
Q: please calculate the unknown concentration of the protein A wih an absorbance value of A188 given…
A: Absorbance = -log (I / Io) where I is intensity of transmitted light and Io intensity if incident…
Q: 1. How many individual proteins do you have if you are able to purify 350 ng of a 267 amino acid…
A: Protein is a nitrogenous organic macromolecule that is necessary for human health. It is responsible…
Q: Calculate the number of kcal and amount of protein provided by the following formula: 500 mL of 50%…
A: Energy is important for functioning of a living organism. It can be obtained from various sources…
Q: . Which test can be used to show up to what stage the hydrolysis of a protein proceeds? Why?
A: Proteins are formed from the polymers of amino acids containing charge on them. Amino acids are…
Q: How would you demonstrate whether a GAG chain assembles from the reducing end versus the nonreducing…
A: GAG is negatively charged heteropolysaccheride made up of repeating disaccharide units,(acidic sugar…
Q: In tabular form explain the specific functions of different of amino acid and how they are acquired…
A: Amino acids are organic compounds that contain amine (–NH2) and carboxyl (–COOH) functional groups,…
Q: 1. Why ninhydrin is used in paper chromatography? 2. How do you identify amino acids on the…
A: Chromatography is method of physical separation of the compound. Chromatography is based on the…
Q: The elution of a protein with an isoelectric point of 7.5, is mostly likely to be affected by a…
A: Chromatography refers to the separation technique that separates substances present in a mixture.…
Q: 1) If a mutation caused an alanine to turn in glycine, what kind of effect do you think that would…
A: Alanine: An aliphatic amino acid with methyl (-CH3) group side chain and is non-reactive side chain…
Q: Q 01 (1 pt.): A variation of centrifugation where the solution in the centrifuge tube varies in…
A: The polypeptide is composed of a long chain of amino acids, which are joined by the peptide bond.
Q: Proteins denature at low pH. What characteristic of acidic environments interferes with protein…
A: Proteins are composed of polypeptide, which exhibits incorporation of amino acids. The quaternary…
Q: 14 Report the approximate pK values for the amino acid whose titration curve is shown below. PH 7…
A: When an amino acid is titrated, the functional group is neutralized from a low Pk value to a high…
Q: How will the temperature affect the sequence of proteins when ribosome stalling is done in vivo? 2…
A: Introduction :- Ribosome are a part of protein synthesizing machinery ( includes mRNA , tRNA ,…
Q: . What is N-glycan microheterogeneity?
A: N glycosylation is considered as one of the most important post translation modification in…
Q: 1. Which of the following statement/s is/are TRUE for the protein sample?* The sample will give a…
A: Part 1. The Biuret test is used for testing the presence of peptide bonds and can also tell the…
Q: If Kd= 100 nM, [RNA] = 10 nM, and [protein] = 1 uM a) What fraction of RNA is bound? b) What…
A: The interaction between the protein and RNA occurs through hydrogen bonding, electrostatic and…
Q: 1. Doesn't non-fat milk protein contain casein which interacts with the Bradford reagent. why would…
A: Bradford and biuret reagent reacts with protein andg ives a visible colour.
Q: State if/how Remedios' mutation changes the amino acid sequence and describe the effect that change…
A: CAT codes for HISTIDINE. CGT codes for ARGININE Since the mutation has changed the final amino acid…
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A: Casein is the major protein component found in milk. It is generally a phosphoprotein which is…
Q: Now consider three sugars-glucose, glucuronic acid, and N-acetylglucosamine. Use shorthand (e.g.,…
A: A glycosidic bond is formed between 2 carbon atoms as the result of a dehydration reaction. The…
Q: 1) what is the net charge of the amino acid leucine of it is on the C-terminus end and why? 2) if…
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- A dry powder that is reconstituted with saline is _______________Acetaminophen (Drug Monograph) In the identification test using infrared absorption, name the reagent where the specimen should be suspended.Answer the following questions (not more than 5 sentences/question). Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.
- Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) The computed amount of powdered ammonium sulfate was added to the egg white sample portion byportion with constant stirring while submerged in an ice bath. The solution is expected to become moreturbid, and a white precipitate is expected to form. The resulting mixture was then filtered using a cheesecloth. The residue was discarded, and 30.0 mL of thefiltrate was brought from 40% to 60% saturation by adding the required amount of powdered ammoniumsulfate in the same manner as the previous addition. After adding ammonium sulfate, the mixture was allowed to stand with occasional stirring for 30 minutes inan ice bath. The formation of a white precipitate is expected to happen QUESTION: Explain the significance of allowing the mixture to be submerged in an ice bath for a given time.The table below summarizes the results for Lead-sulfide test. Provide the correct remarks from the results if Negative for Lead-sulfide test or Positive for the Lead sulfide test and it's either Negative for -SH or Positive for -SHExperimental Procedures 1. Obtain five unknown lipid samples from your laboratory instructor. 2. Place approximately 2 ml of each sample in separate test tubes. Use fresh samples for each qualitative test. 3. For copper acetate test, add 2 ml of the test reagent to the sample and heat in a water bath for 3-5 minutes. Observe and record the color change. 4. For acrolein test, add 2 scoops of KHSO4 to the sample using a spatula. Heat the samples vigorously through a Bunsen burner or alcohol lamp for 3-5 minutes. Carefully smell the sample afterwards by wafting of the vapor to your nose. A sharp irritating odor indicates a positive reaction. 5. For Hubl's test, add 5 drops of the test reagent to the sample and shake the mixture thoroughly for 3 minutes. Observe and record the color change. 6. For ammonium molybdate test, add 2 ml of concentrated nitric acid and 5 drops of ammonium molybdate reagent to the sample. Heat the mixture for 3-5 minutes in a water bath. Observe for formation of…
- Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possibleWhich among the following statements is correct? O A positive result for Fohl's test is the formation of a black precipitate in the form of lead. O A positive result for Millon's test is characterized by the formation of a red-colored precipitate or solution. O Both statements are correct Both statements are incorrectThe usual dose of digoxin for rapid digitalization is a total of 1.0mg, divide into two or more portion at intervals of 6 to 8 hours. How many milliliters of digoxin elixir containing 50mcg/mL would provide this dose?
- The solution was graded as incorrect in my homework. Is there another option for this question?Question Answer the following questions. Please I really need help. Even if you write something easy, I beg you.Explain in your own words how the load test (performance test) is applied to the patient turning apparatus shown in the figure, and guess how the expected result will be. The main part of the product consists of a polyurethane sponge. Stain-proof upholstery fabric will be used as the outer surface material. In addition, there are heating pads working with the logic of electric blankets in this apparatus, please answer by taking these into account when answering.Label the key components of a matrix-assisted laser desorption/ionization time-of-flight, or MALDI-TOF, mass spectrometer. ion source matrix laser Answer Bank detector transient recorder Beam splitter Trigger flight tube O protein sample