6. A fellow classmate comes to you to ask your opinion about the following result from this SIM test: a. Provide a full interpretation and analysis of the results this test SIM medium detects 3 properties of an organism. Indole production, Motility and H2S production. Indole production is indicated by production of red ring on addition of Kovacs reagent. Here red ring is in the tube which indicates the organism is Indole positive. Motility is detected by diffuse growth. Here diffuse growth is seen which indicates the organism. Presence of black color indicates H2S production. Here black color is produced which indicates H2S is produced. b. Your classmate is not fully convinced about the black coloring seen in this result. Please suggest another type of method that would help confirm this result and what explain what the result should show. KIA test can be used to detect H2S production. KIA contains sodium thiosulfate as sulfur source and ferrous sulphate act as a H2S detector. Black deep butt indicates H2S production

6. A fellow classmate comes to you to ask your opinion about the following result from this SIM test: a. Provide a full interpretation and analysis of the results this test SIM medium detects 3 properties of an organism. Indole production, Motility and H2S production. Indole production is indicated by production of red ring on addition of Kovacs reagent. Here red ring is in the tube which indicates the organism is Indole positive. Motility is detected by diffuse growth. Here diffuse growth is seen which indicates the organism. Presence of black color indicates H2S production. Here black color is produced which indicates H2S is produced. b. Your classmate is not fully convinced about the black coloring seen in this result. Please suggest another type of method that would help confirm this result and what explain what the result should show. KIA test can be used to detect H2S production. KIA contains sodium thiosulfate as sulfur source and ferrous sulphate act as a H2S detector. Black deep butt indicates H2S production

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter30: Assisting With Minor Surgery

Section: Chapter Questions

Problem 2CR

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of 1 stion s page 30- F2 Which of the following terms is associated with the probability of a preparation not being sterile after it has been exposed to a sterilization process? Select one: Oa. Particulate Matter level Qb. Sterility Assurance level О с. Pyrogen Load level O d. Optimal Sterilization level F3 F4 F5 Ol پیار Q Search F6 F7 F8 F9 === 8 9 F10 Time left 22:34:03 F11 Next page
1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.
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1 H 2 176 old culture Smear too BOTERC Losk like? + b. Failure to apply the decolorizer. + ne c. Failure to apply the safranin. Determine whether bacteria are motle or non-motle by looking at the growth in semi-solid medium What is the concentration of agar in (a) typical solid medium (b) semi sold motility medium? Arawer Q1 on Page 357. 7. Orygen Requirement: + d. Reversal of crystal violet and safranin stains. A questions 1.2 and 3 o Pg 83-84 Refer to the power point Questions 1 Predict the effect of the following "mistakes" made when performing a Gram stain. Your answer needs to address the predicted effect on both Gram-positive and Gram-negative cells. Consider each mistake independently. a. Failure to add the iodine. 414 gait to fer. 2 Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram-positive and Gram-negative cells. This being the case, explain how they stain different cell types in the Gram stain. why? a cell wall. patem in…