(a) A sample of body serum is to be analysed for sodium by flame emission spectroscopy. 1.00 cm3 aliquot of serum was pipetted into each of two 50.0 cm3 volumetric flasks. The first flask was diluted to volume with deionised water. The absorbance of this first solution was 0.350. To the second flask 10.0 cm3 of a 25.0 ppm sodium standard was added and the flask made up to volume with deionised water. The absorbance of this second solution was 0.720. (i) Calculate the concentration of the sodium in the body serum in mg dm–3

(a) A sample of body serum is to be analysed for sodium by flame emission spectroscopy. 1.00 cm3 aliquot of serum was pipetted into each of two 50.0 cm3 volumetric flasks. The first flask was diluted to volume with deionised water. The absorbance of this first solution was 0.350. To the second flask 10.0 cm3 of a 25.0 ppm sodium standard was added and the flask made up to volume with deionised water. The absorbance of this second solution was 0.720. (i) Calculate the concentration of the sodium in the body serum in mg dm–3

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter14: Applications Of Ultraviolet-visible Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 14.9QAP

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A sample of body serum is to be analysed for sodium by flame emission spectroscopy. 1.00cm' aliquot of serum was pipetted into each of two 50.0cm' volumetric flasks. The first flask was diluted to volume with deionised water. The absorbance of this first solution was0.350. To the second flask 10.0 cm' of a 25.0 ppm sodium standard was added and the flask made up to volume with deionised water. The absorbance of this second solution was 0.720.(i) Calculate the concentration of the sodium in the body serum in mg dm-3.
Define mass spectroscopy and the underlying principle/background of its method for characterizing samples.
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