A heptapeptide that puncture the bacterial cell wall has just been recently isolated from the venom gland of a frog. Use one letter code of amino acid: What is the C-terminal residue? What is the N-terminal residue? What is the sequence of Peptide 3? What is the sequence of Peptide 5? What is the over all amino acid sequence?

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter21: Heparin Infusion Calculations
Section: Chapter Questions
Problem 1.3P
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A heptapeptide that puncture the bacterial cell wall has just been recently isolated from the venom gland of a frog.

Use one letter code of amino acid:

What is the C-terminal residue?

What is the N-terminal residue?

What is the sequence of Peptide 3?

What is the sequence of Peptide 5?

What is the over all amino acid sequence?

Hydrazine
Lys and
modified free
amino acids
Heptapeptide
Acid Hydrolysis (6 N HCI) at 110 °C
2,4-dinitrofluorobenzene,
Cleavage with Trypsin, then chromatography
Cleavage with Chymotrypsin, then chromatography
(2) Arg, Pro, Phe, Tyr, Ala, Lys
DNP-Phe
Peptide 1
(Ala, Lys)
Peptide 2
(Pro, Arg, Tyr)
Peptide 3
(Phe, Arg)
Phe
Peptide 4
(Arg, Tyr)
Peptide 5
(Lys, Pro, Ala, Arg)
Transcribed Image Text:Hydrazine Lys and modified free amino acids Heptapeptide Acid Hydrolysis (6 N HCI) at 110 °C 2,4-dinitrofluorobenzene, Cleavage with Trypsin, then chromatography Cleavage with Chymotrypsin, then chromatography (2) Arg, Pro, Phe, Tyr, Ala, Lys DNP-Phe Peptide 1 (Ala, Lys) Peptide 2 (Pro, Arg, Tyr) Peptide 3 (Phe, Arg) Phe Peptide 4 (Arg, Tyr) Peptide 5 (Lys, Pro, Ala, Arg)
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