A mutant B cell line is examined by confocal microscopy after incubation with a microbial pathogen recognized by the BCR on these B cells. The B cells have been stained with antibodies to visualize the localization of polymerized actin and microtubules. As a control, wild-type B cells are examined. The results are shown in the figure below, with the numbers indicating the proportion of cells examined that show each pattern of staining. To identify the specific signaling defect in these mutant B cells, a reasonable biochemical assay would be to:Determine if BCR stimulation of mutant B cells produces enhanced binding of the B cell to the microbeDetermine whether the mutant B cells have reduced levels of the enzyme Protein kinase C-qDetermine whether the mutant B cells are overexpressing the enzyme VavDetermine whether BCR stimulation of mutant B cells promotes exchange of GDP for GTP on cdc42Determine whether BCR stimulation of mutant B cells produces increased levels of DAG Wild-type B cellMutant B cells33%microtubules andactin polymerization33%98%microbeВ сell33%

“Major histocompatibility complex” is an acronym for “major histocompatibility complex.” It refers…

Answered: A mutant B cell line is examined by…

BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)

10th Edition

ISBN:9781305967359

Author:STARR

Publisher:STARR

Chapter34: Immunity

Section: Chapter Questions

Problem 5SA: Activated complement proteins ________. a. puncture cells c. attract macrophages b. promote...

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A mutant B cell line is examined by confocal microscopy after incubation with a microbial pathogen recognized by the BCR on these B cells. The B cells have been stained with antibodies to visualize the localization of polymerized actin and microtubules. As a control, wild-type B cells are examined. The results are shown in the figure below, with the numbers indicating the proportion of cells examined that show each pattern of staining. To identify the specific signaling defect in these mutant B cells, a reasonable biochemical assay would be to:

Determine if BCR stimulation of mutant B cells produces enhanced binding of the B cell to the microbe
Determine whether the mutant B cells have reduced levels of the enzyme Protein kinase C-q
Determine whether the mutant B cells are overexpressing the enzyme Vav
Determine whether BCR stimulation of mutant B cells promotes exchange of GDP for GTP on cdc42
Determine whether BCR stimulation of mutant B cells produces increased levels of DAG

Wild-type B cell
Mutant B cells
33%
microtubules and
actin polymerization
33%
98%
microbe
В сell
33%

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

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