A solution of Tryptophan has an absorbance at 280 nm of 0.25 in a 0.5 cm length cuvette. Given the absorbance coefficient of Tryptophan is 6.4 × 103 L Mol-1 cm-1. What is the concentration of solution?

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Q: absorbance

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Q: Can you help me on questions 2 AND 3

A: To solve this problem we can use beer’s law equation:

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.