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- (a) Three components in a mixture have varying distribution constants between the mobile phase and the stationary phase. One of the components has a very high distribution constant. Would this component likely elute first or last and how would you design an experiment or modify the components of the separation to make it perform in the opposite manner? (b) One way to dramatically increase the sensitivity of a quadrupole mass spectrometer is to use a technique called selected ion monitoring (SIM). Explain how this technique works and why it improves sensitivity. Explain what dwell time means. Be sure to draw a diagram and describe how this analysis scheme works. Also, is there a compromise between a wide range of masses scanned and SIM? Explain in easy details.(2) Adenine has a molar absorbance of 4 13.1 M-1cm-1 for 263 nm nucleic acid. What is the molar concentration of an acid solution that shows a permeability of 75% when 2 ml of it is placed in an absorption cell with a thickness of 1 cm?2. Answer ALL parts. (a) The absorbance of compound A was measured in a 1.00 cm cuvette at 238 nm. A 3.96 x 10 M solution of compound A exhibited an absorbance of 0.624. A blank solution containing only the solvent had an absorbance of 0.029 at the same wavelength. Calculate the molar absorptivity of compoundA in m' mole". (i) The absorbance of an unknown solution of Compound A in the same solvent and cuvette was 0.375 at 238 nm. Find the concentration of A in the unknown. (iii) A concentrated solution of Compound A in the same solvent was diluted from an initial volume of 2.00ml to a final volume of 25 ml and then had an absorbance of 0.733 under the same conditions. What is the concentration of A in the concentrated solution? (iv) What type of cuvette was used in the above analysis? Justify your answer. (b) Name ONE advantage and ONE disadvantage of a linear photodiode array detector compared to a traditional photomultiplier based spectrometer. (c) Why is an FTIR spectrum digital in…
- Figure 7. (a) Plots of Prepared versus Calculated percentages of p-aminophenol mixed with acetaminophen determined using (a) the Raman peak height at 846 cm ; and the 550-1800 cm Raman spectral region using (b) raw spectra (circles), and (c) first derivatives of the spectra (squares). Ideally, the slope should equal 1.0 and the intercepts 0.0. (a) (b) Prepared Percent Prepared Percent Explain the above drawing in detail and why it appeared in this way, and what the axes represent in the drawing, what is the relationship between the two axes, and why the drawing appeared in this way. What do the numbers in the figure represent? Calculated Percent RSRR Calculated PereentCalculate the concentration of a solution of peptide AWSME, in mM if the absorbance at 280 nm for the solution is 0.6 absorbance units, and a 0.2-cm cuvette is used. For the extinction coefficient, take into account that e280(Trp) = 5690 M-1 cm-1 and e280(Tyr) = 1280 M-1 cm-1. Round your answer to two decimals.(a) You measure the percent transmittance of a solution containing chromophore X at 400nm in a 1-cm path length cuvette and find it to be 50%. What is the absorbance of this solution? (b) What is the molar absorptivity of chromophore X if the concentration of X in the solution measured in question (a) is 0.5mM? (c) What is the concentration range of chromophore X that can be assayed if, when using a sample cell of path length 1, you are required to keep the aborbance between 0.2 and 0.8?
- Atomic Spectroscopy (a) In terms of spectral shape, how are atomic spectra different from molecular spectra? What is the source of this difference? (b) Describe the decision-making process for picking a line for atomic emission analysis of a given analyte. (c) For quality assurance, spike recovery is not sufficient to establish confidence in an analytical procedure. Explain this statement. What should be done instead to demonstrate satisfactory performance of an instrumental method (including elemental analysis). (d) Find a literature example of atomic spectroscopy used for elemental analysis. State the operating conditions with sufficient detail to reproduce the analysis; Please answer very soon will give rating surely All questions complete Answer needed Please help meAs the pH changes, how will the absorbance change at the wavelength of the isosbestic point?(b) What are cross-peaks? Discuss the are cross-pcaks? Discuss the characteristics of cross-peaks of COSY and NOESY.
- What are the necessary conditions for a metal isotope to be useful as a sources in mossbauer spectroscopy.The ESI mass spectrum of the protein transferrin gave a regular series of peaks, with the most intense peaks having the following m/z values.(i) Suggest a likely reason for the observation of these peaks in the ESI mass spectrum of the intact protein. (ii) Use the above data to calculate the molecular weight of transferrin. Show your working and state any assumptions made."[FeSCN2+]eq is calculated using the formula: [FeSCN2+]eq = Aeq/Astd x [FeSCN2+]std where Aeq and Astd are the absorbance values for the equilibrium and standard sample respectively." I am confused about what Aeq and Astd mean. We did an experiment where we measured the absorption of this chemical formation and I got around 0.18. Is that one of the values?