A. FERMENTATION OF CANE SUGAR 1. In a 500 mL Florence flask, place 50 gms of brown cane sugar and 1 gram of disodium phosphate. 2. Dissolve 1 teaspoon of yeast in 15 mL of water. Add this to the sugar in the flask together with 150 mL water. Stir until the sugar is dissolved. 3. Close the flask with a rubber stopper provided with a delivery tube so that the evolved gas will bubble through a solution of clear limewater. 4. HAVE THE SET – UP CHECKED BY YOUR INSTRUCTOR. 5. Keep the set – up inside the locker for 5 – 6 days at room temperature. Fermentation Set – up: ________________________________ Write the equation for the hydrolysis of cane sugar _____________________________ Write the equation for the fermentation of the simple sugars to alcohol __________ 6. At the end of the fermentation period, what is formed in the flask containing limewater? _________________________________________________________________ Write the equation for its formation ____________________________________________ C. PROPERTIES a. Combustibility: Pour a few drops of the distillate on a watch glass and ignite. Describe the result. ___________________________________________________________ b. Presence of Water: 1. Heat 1 gm of finely pulverized copper sulfate gently in an evaporating dish. Describe the result: ___________________________________________________________ 2. Place a few drops of the distillate in the heated copper sulfate. Describe the result: ___________________________________________________________ Property of and for the exclusive use of SLU. Reproduction, storing in a retrieval system, distributing, uploading or posting online, or transmitting in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise of any part of this document, without the prior written permission of SLU, is strictly prohibited. 9 c. Iodoform Test for Ethyl Alcohol 1. To about 5 mL of the distillate in a test tube, add 2 or 3 crystals of Iodine. 2. Then add 3 mL of 8 N sodium hydroxide solution. 3. Warm the mixture for 5 minutes in a water bath at 60 deg C. 4. Shake occasionally and allow to stand. Describe the result. _____________________ Write the equation for the formation of iodoform from ethyl alcohol. _____________
A. FERMENTATION OF CANE SUGAR
1. In a 500 mL Florence flask, place 50 gms of brown cane sugar and 1 gram of
disodium phosphate.
2. Dissolve 1 teaspoon of yeast in 15 mL of water. Add this to the sugar in the flask
together with 150 mL water. Stir until the sugar is dissolved.
3. Close the flask with a rubber stopper provided with a delivery tube so that the
evolved gas will bubble through a solution of clear limewater.
4. HAVE THE SET – UP CHECKED BY YOUR INSTRUCTOR.
5. Keep the set – up inside the locker for 5 – 6 days at room temperature.
Fermentation Set – up: ________________________________
Write the equation for the hydrolysis of cane sugar _____________________________
Write the equation for the fermentation of the simple sugars to alcohol __________
6. At the end of the fermentation period, what is formed in the flask containing
limewater? _________________________________________________________________
Write the equation for its formation ____________________________________________
C. PROPERTIES
a. Combustibility: Pour a few drops of the distillate on a watch glass and ignite.
Describe the result. ___________________________________________________________
b. Presence of Water:
1. Heat 1 gm of finely pulverized copper sulfate gently in an evaporating dish.
Describe the result: ___________________________________________________________
2. Place a few drops of the distillate in the heated copper sulfate.
Describe the result: ___________________________________________________________
Property of and for the exclusive use of SLU. Reproduction, storing in a retrieval system, distributing, uploading or posting online, or transmitting in any form or by any
means, electronic, mechanical, photocopying, recording, or otherwise of any part of this document, without the prior written permission of SLU, is strictly prohibited.
9
c. Iodoform Test for Ethyl Alcohol
1. To about 5 mL of the distillate in a test tube, add 2 or 3 crystals of Iodine.
2. Then add 3 mL of 8 N sodium hydroxide solution.
3. Warm the mixture for 5 minutes in a water bath at 60 deg C.
4. Shake occasionally and allow to stand. Describe the result. _____________________
Write the equation for the formation of iodoform from ethyl alcohol. _____________
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