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- 6) You are given a mixture of organic compounds suspected of containing various amines (e. g. 4-chloroaniline). What is the best way to quickly separate these amines from the other organic compounds? a) Perform a fractional distillation on the mixture. b) Benchtop silica gel column chromatography. c) Dissolve the mixture in an organic solvent and treat the solution with aqueous HCI. d) Dissolve the mixture in an organic solvent and treat the solution with aqueous NaOH.Need help with a and b. A mixture of 1-hexanol, hexane, and 1-chlorohexane is injected on a gas chromatography column. a) Predict the sequence of retention times indicating which compound will have the shortest, middle, and longest retention time. b) The sample vial of (a) was not carefully sealed and left partially open for a couple hours. A GC is taken again with only two peaks realized. The first peak to appear has an area of 24 mm2 and the second peak 40 mm2. What mole% of each compound (specify the compound name) is most likely present?Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.
- A gas chromatography column containing a poly(dimethylsiloxane) stationary phase is used to separate the molecules listed. Place the molecules in the order they will elute from the column. Refer to a list of retention indexes for several molecules. Elutes first hexane benzene heptane 2-pentanone 1-nitropropane octane Elutes last Anawer Bankseparate a mixture containing acetone, acetamide and 1,2-dichloroethane using a high- performance liquid chromatography (HPLC) technique. The instrument was equipped with a 40-cm column packed with cyano propyl bonded to the siloxane backbone material. i) List THREE (3) examples of mobile phase solvent/solvent systems that can be used with the column above. ii) Determine the sequence in which the three analytes eluted from the column and explain the underlying principle applied. iii) Sketch the chromatogram that would be produced if the column was replaced with a column filled with C18 hydrocarbon bound to the siloxane backbone material and acetonitrile was employed as the mobile phase. Label the peaks observed.A student places an unknown sample on a thin-layer chromatography plate and develops it in dichloromethane as a solvent. Only one spot whose Rf is 0.98 is observed. Does this result indicate that the unknown material is a pure compound? If not a pure compound, suggest an alternate procedure to verify the purity of the sample.
- A mixture of three compounds was chromatographed using a silica gel column with diethyl ether/hexanes (10:90) as the eluting solvent. Fractions were collected and TLC analysis of each fraction was performed, with the results shown here: A 3 4 7 8 Mixture before separation Which fractions should be combined to give pure samples of A, B and C?You are attempted to purify a compound via column chromatography. Before starting the process, you determine the eluent solvent conditions using TLC. Upon running a TLC you determine the following: 1) In pure hexanes the Rf of your desired compound is 0.10 and the major impurity Rf is 0.15. 2) In pure ethyl acetate the Rf of your desired compound is 0.95 and the impurity comes to the eluent line. 3) in 90:10 hexanes:ethyl acetate the Rf of your desired compound is 0.40 and the impurity at 0.90. Which of these eluents would you use to run your column? Neat hexanes Neat ethyl acetate 90:10 hexanes:ethyl acetate O WaterA mixture contains the following compounds: 2-chloropentane, pentanoic acid, cyclopentane and pentanal. Arrange the following compounds in INCREASING Rf if the mixture underwent paper chromatography with the same solvent system used in the experiment.
- Look up the structures of naphthalene and benzoic acid and determine their polarities. Using the polarity of the molecules, predict which would elute first and which would elute second on a C18 column in reverse phase chromatography.A thin-layer chromatography (TLC) experiement was run using three compunds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with the three compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?Look up the boiling point for each of the following compounds then determine the order that they will elute from the column. Use this elution order to identify the compound in each of the 6 standard chromatograms. Once you have identified the compound in each chromatogram, record its retention time. Compound Structure Compound Name Elution Order (1-6) Non polar → Polar Boiling Retention Time (From Chromatograms) Point Propyl acetate H H. H. Butyl acetate H. H. H H. H H. H. H. Methanol H O- Ethanol 2-butanone H H H. H. 4-methyl-2- pentanone H. H. HH H.