About transcriptomics and genomics, answer: 4. Can we replicate RNA? How can we use PCR and Sanger sequencing to study the ranscriptome? a. b. How will the genome of two different cells of the same animal compare? How will the transcriptome oftwo different cells of the animal compare? c.
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- a. What is the purpose of molecular cloning?b. What purpose do selectable markers serve in vectors?c. What is the purpose of the origin of replication in aplasmid vector?d. Why do cloning vectors have polylinkers?A. What are some reasons a person might want to clone a human? B. What animal was cloned in 1885?What is the most challenging issue facing genome sequencing? a. the inability to develop fast and accurate sequencing techniques b. the ethics of using information from genomes at the individual level c. the availability and stability of DNA d. all of the above
- Short tandem repeats (STR) profiling is based on O A. the fact that many foods are being genetically modified and this test allows food health officials to identify the transgenic ones. O B. the fact that you can clone mammals through fusion of one somatic (non-sex) cell with an egg cell whose nucleus has been removed O C. the fact that one strand of DNA can be turned into millions of identical copies by a process that heats and cools DNA and builds it using DNA polymerase and primers. OD. the fact that people's DNA is filled with short sequences, like "TCAT" that are found in different numbers in each person.Q. How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and reverse primers that bind to the same exon. B. Run a control lane where only RT was performed and not PCR. C. Run a control lane where mRNA has been amplified using PCR. D. Use forward and reverse primers that span the junction of 2 separate exons.A. In what year was the National Bioethics Advisory Council formed? B. The first mammal clone to be produced from an adult (somatic cell)?
- 1. Through observing the sequence of events (A → B → C → D) found below, describe how reproductive cloning is made possible. Your response should consist of 3—5 complete sentences. SENTENCE STARTER: The process of cloning may look quite tricky, but it can actually be broken down into 4 individual steps. In step A, the cells of the organism we want to be cloned are isolated and the… 2. What is an ADVANTAGE of engaging in cloning practices similar to the one highlighted above? What reason might a farmer, livestock owner, or someone else use as justification for cloning? 3. What is a DISADVANTAGE (or potential drawback) of cloning plants, livestock, or pets?a. What determines the size (length) of the primary PCR product? b. What might a successful gel check of a PCR reaction look like?A has been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a. Transgenic genome b. Recombinant DNA sequence c. Knockdown gene d. Synthetic genome o e. Recombinant plasmid Clear my choice is changing our Sequencing the human genome, the development of microarray technology, and understanding of complex diseases like cancer. They help us to observe the gene expression patterns in genetic disease by comparing the healthy tissue of individuals against the disease state of others. Select one: C a. Proteomics o b. Metagenomics MO C. Functional genomics d. Personal genomics O e. Developmental genomics Clear my choice
- Which of these conditions are always true of populations evolving due to natural selection? Condition 1: The population must vary in traits that are heritable. Condition 2: Some heritable traits must increase reproductive success. Condition 3: Individuals pass on most traits that they acquire during their lifetime. Select one: O a. Conditions 2 only O b. Conditions 1 and 2 Conditions 2 and 3 Conditions 1 only O c. O d.For each of the following scenarios, indicate YES (it is cloning) or NO (it is not cloning). 6. ___________ Sperm taken from a male goat is combined with a female's egg in a petri dish. The resulting embryo is implanted into the female's uterus to develop 7. ___________ A sheep embryo, composed of 16 cells, is removed from the mother's uterus and separated into individual cells. Each cell is allowed to multiply, creating 16 separate embryos, which are then implanted in different female sheep to develop to maturity. 8. ___________ A cow with many desirable traits is stimulated with hormones to produce a number of egg cells. Each of these eggs is fertilized and implanted into a surrogate mother. 9. ___________ Cell nuclei from a recently deceased dog are placed into enucleated egg cells from another female dog. These egg cells are then placed into the uterus of an additional female surrogate dog, where it grows into a puppy.1. What two restriction sites are you going to use to clone your PCR product into the pL4440 plasmid? What are their DNA sequence? 2. State the primer sequence you will use to amplify the F27C1.7 gene ready to be cloned into the pL4440 plasmid? 3. How would you go about cloning this amplified DNA into pL4440? Using your knowledge of cloning list 5 important aspects of the method.